1. G.GURGENIDZE, M.GZIRISHVILI, D.LABARTKAVA, M.MIKABERIDZE, N.URUSHADZE
CONSTITUTIONAL ANOMALIES IN THE PATIENTS WITH ATOPIC DISEASES AND THE WAYS OF THEIR CORRECTION
J. Experimental and Clinical Medicine, 2005, 3, pp.11-14.
Institute of Medical Biotechnology of the Georgian Academy of Sciences, Tbilisi, Georgia
The examination of the persons diseased by atopic bronchial asthma has vividly revealed that the mentioned clinico-pathogenetic form is associated with a constitutional type of anomaly: in particular, there is observed the increase of immunoglobulins with reagent activation effect and the decrease of G and M class of immunoglobulins that is mainly conditioned by a quantitative deficit of immune regulative lymphocyte clones and their qualitative dysfunction.
2. M.KAKABADZE, N.CHCHINADZE, N.DIDEBULIDZE
RADIOMETRIC PARAMETERS OF ESTROGEN DISTRIBUTION IN THE MYOCARDIUM
OF FEMALE RATS
J. Experimental and Clinical Medicine, 2005, 3, pp.14-17.
A. Natishvili Institute of Experimental Morphology of the Georgian Academy of Sciences, Tbilisi
The results of lots of clinical observations and experimental investigations have revealed the relation between sexual glands and myocardium functional state. The cells sensible to these hormones permanently require a supporting quantity of them for normal functioning.
The aim of our investigation was to determine the distribution of estrogen receptors in the cardiac muscle of intact and castrated rats. For this reason 30 white rats were used.
To discover the distribution of estrogen in the myocardium of the experimental animals, the radiometrical method has been used. We defined the maintenance of radioactive impulses of synthetic steroids-3H-moxestrol in 1g of heterorganic homogenate of the heart tissue, which was taken in the auricle of the atria, auricle and ventricle.
The radiometric analysis of our studies has shown that radioactive estrogen is distributed among different areas of the heart (auricle of atria, auricle and ventricle). This suggests that all the above-mentioned areas of the myocardium have specific estrogen receptors in the cells. We think that expression of the distribution of impulses in the ventricles is connected with synthetic estrogen (3H Moxestrol) that we have used in our studies. It is known that 3H Moxestrol does not bind with blood proteins and is characterized by a high specificity of receptors binding [3].
Estrogen deficiency induced by ovariectomy has influenced the estrogen depended receptor system of cardiomiocytes. The quantity of unbounded receptors in the myocardium has increased due to ovariectomy that lead to the sharp decrease in the endogenous estrogens. On the other hand, this circumstance lead to the binding of the unbounded receptors by 3H Moxestrol. It is necessary to note that there is a possibility of the development of novel estrogen receptors that confirm that the myocardium needs a definite level of estrogen for promoting the compensatory adaptive mechanism.
3. IMAMVERDIYEV S.B., ABU-NAHLEH KHALED
OPERATIVE TREATMENT OF HYDRONEPHROSIS DURING STENOSIS OF PELVIC-URETERAL SEGMENT
J. Experimental and Clinical Medicine, 2005, 3, pp.17-23.
The Department of Urology of the Azerbaijan Medical University, Baku
For the period from 1990 to April 2005 115 patients aged 4-54 (middle age 23.3) were operated apropos of hydronephrosis owing to stenosis of pelvic ureteral segment (PUS). Of them, 38 were women. 25 of 115 patients were persons under 16 years, in 27 ones besides stenosis of PUS were detected single (15 patients) , multiple (7 ones) coralshaped (2 ones) , multiple and coralshaped (3 ones) calculuses.
In the presence of the additional vessels artevasal pyelo-pyeloanastomosis was carried out in 29 patients , urethra-pyeloanastomosis after resection of PUS because of its stenosis was carried out in 11 ones. Fenger’s operation was carried out in 5 patients. PUS resection with urethra-pyeloanastomosis was carried out in 66 patients. The operation was limited by the crossing of the additional vein in 3 patients that is in 1 patient their pelvis with reimplantation of the ureter with funnel out of the pelvis into the stoping part of one according to the type - the end inside . The operation was carried out in 2 patients on half of a caked kidney (one by istmotomy). Anastomosis was put by catgut 4/0 or vikril 5/0 uninterruptedly. The secondary nephroectomy was carried to 1 patient because of stenosis relapse. The coralshaped calculus was formed in 1 patient (anastomosis was put by vikril) in 3 years. The distant results from several months to 13 years were observed on more than 60% patients besides the mentioned ones. The complications were absent.
On the whole, the operations results are highly satisfactory.
ALLOGENIC HEPATIC CELL TRANSPLANTATION IN TREATMENT OF HEPATIC INSUFFICIENCY
J. Experimental and Clinical Medicine, 2005, 3, pp.23-26.
Department of Clinical Anatomy, Georgian State Medical Academy
An increase in patients with liver diseases results in the growth of morbidity caused by liver insufficiency. A comparative analysis of the cells and efferent methods (hemosorption, hemofiltration, plasmaphresis, liver cell transplantation) in the treatment of hepatic failure showed that the mortality rate for patients continues to be high.
The results of the treatment of animals suffering from acute toxic affection of the liver indicate a high therapeutic effectiveness of such dialysis. Cellular peritoneal dialysis did not induce anaphylactic reactions in animals. A favorable dynamics of changes noted in the biochemical, clinical and morphological indices allows this method to be recommended for clinical approbation.
J. Experimental and Clinical Medicine, 2005, 3, pp.26-28.
It is known that complications in newborns and infants with pyloric stenosis are found before the surgical intervention, as well as during and after it.
As a rule, preoperative complication causes the difficulty during operative and postoperative course of the disease. Accordingly, they increase the probability of mortality.
We set our minds on suggesting and elaborating a number of recommendations on the prophylaxy of possible complications of pyloric stenosis in newborns and infants on the basis of the literary data and careful analysis of 120 patients’ medical histories from our clinical experience.
According to our profound conviction, the exact observance of these recommendations will minimize the undesirable and sometimes even tragic consequences of complications of the given disease.
J. Experimental and Clinical Medicine, 2005, 3, pp.28-31.
A.Natisvili Institute of Experimental Morphology Georgian AS, Tbilisi, Georgia
It was found that the expression of the Ki-67 and CD31 in the tumor tissues is variable and depends on cancer histogenesis, histological form, differentiation and stage of tumor development.
The rate of the expression of both markers is significantly higher in ductal cancers thn in lobular ones.
Ki-67 and CD31 markers expression is controversial in the cancers with different histological forms. It is higher in the medullar, comedo and solid tumors than in the papillary and tubular forms.
Markers expression rate grow up with correlation of the tumor anaplastic characteristic. Some significant data are received about markers expression in the different age groups.
There was found, correlation between Ki-67 and CD31 markers expression which depends on cancer histogenesis, histological form, differentiation and stage of tumor development.
7. T.MACHAVARIANI, I.GACHECHILADZE, K.KAVTIASHVILI, S.MAMPHORIA, E.JANBERIDZE, I.LACABIDZE
STRUCTURAL CHANGES IN PANCREAS ISLETS DURING ALLOXAN DIABETES AND AFTER ACTION OF PLAFERON
J. Experimental and Clinical Medicine, 2005, 3, pp.31-34.
A. Natishvili Institute of Experimental Morphology of the Georgian Academy of Sciences
Structural changes were investigated in the pancreas islets on the experimental model of alloxan diabetes (AD) in underbred dogs by the histological, histochemical, electron microscopy and electro-radioautographical methods at various times of the experiment (2 weeks, 6 month, 12 month) and at different degrees of AD.
During alloxan diabetes at the later time (12 month) there was observed the infiltration of fibroblasts, lymphocytes, plasmocytes, also collagen fibers were signified. After the action of plaferon in the animals, it was detected proliferation of macrophages. The active macrophages cause collagen lysis, and insulin granules easily move toward the capillaries - this is the manifestation of compensatory-adaptative processes.
8. N.T.LORTKIPANIDZE, D.METREVELI
COMBINATION OF ALOPECIA AREATA AND AUTOIMMUNE THYROIDITIS
J. Experimental and Clinical Medicine, 2005, 3, pp.34-37.
Scientific-Research Institute of Dermatology and Venereology, Department of the Dermatology-Venereology of the Chair of Internal Diseases, Medical Faculty of Iv. Javakhishvili Tbilisi State University; Cheir of Endocrinology of the State Medical Academy of Georgia;
ENMEDIC - Centre for Endocrinology, Metabolism & Nutriology
Alopecia areata (AA) is a disorder primarily affecting hair, in which associated autoimmune or atopic disease is common. The aim of the study was to ascertain the reliability of the combination of alopecia and autoimmune tyroiditis. The autoimmune thyroiditis was diagnosed in endocrinology medical center “Enmedic”. Selection of 300 patients was held via the method of simple random sampling (randomization). To analyse qualitative differences we used a c² -test and Fisher’s exact test when appropriate. Alopecia areata was found in one of 300 persons of the control group. Of 300 persons diseased by autoimmune thyroiditis, alopecia areata was present in 12. Autoimmune thyroiditis was significantly associated with alopecia areata (P=0.005; odds ratio (OR)= 12.46; 95% CI 1.68-258.18). In 12 patients with alopecia areata: 3 had alopecia localis (patchy); 6 - totalis (P=0.13; Fisher exact: 1tailed P=0.06), and 3 - universalis. Therefore, 9 patients had an extensive form (P=0.025). These data support a significant association between alopecia areata and autoimmune thyroiditis. There also exists a reliable association between the extensive form of alopecia areata and autoimmune thyroiditis.
9. L.BUTSCHRIKIDZE, I.MUKHADZE, M.DOLIDZE, D.DJINCHARADZE
CANEPHRON IN COMBINATION WITH PHOTONS DURING PYELONEPHRITIS IN PREGNANT WOMEN
J. Experimental and Clinical Medicine, 2005, 3, pp.37-40.
Tbilisi State Medical Academy, Department of Obstetrics and Gynecology ¹1, Georgia
In the submitted work are given the data of the analysis of clinical-laboratory and paraclinical research methods which were used for 69 pregnant patients with gestational pyelonephritis. Along with the standard medical treatment, they received in dynamics photon therapy combined with canephron. We conducted openly controlled, prospective research with parallel control.
The Application of the given complex of preventive-medical measures is an effective, available and simple method allowing to a marked improvement of pregnancy course and outcome.
10. Z.BARTAIA, I.MUKHADZE, N.TSINTSABADZE, D.DJINCHARADZE
PHOTONS AND MAGNE B6 IN THE TREATMENT OF HYPERTENSION DURING PREGNANCY
J. Experimental and Clinical Medicine, 2005, 3, pp.40-42.
In this study there were analized 88 pregnant women with hypertension by dynamic control. They took photons and magne B6 along with the other traditional hypertension treatment methods. There was conducted a prospective study with parallel control. We used the open controller study. It’s very important to find this pathology early and start treatment on an early stage. Our study is very simple and available for everyone, it gives us chance to control pregnancy hypertension and its outcome is better for babies, as well as for mothers.
APPLICATION OF essential fatty acids in treatment of chronic DERMATOSES
J. Experimental and Clinical Medicine, 2005, 3, pp.42-45.
Tbilisi State Medical University, Department of Dermatology, Tbilisi, Georgia
In the pathogenesis of some chronic dermatoses a certain role belongs to the activation of lipid peroxidation and inhibition of the antioxidant system of the organism. The main substrate of this process is unsaturated fatty acids of membrane phospholipids. The structural integrity of cellular membranes in many respects depends on liposoluble vitamins.
The efficacy of systemic and local antioxidants (preparation “Primaderm”) in the treatment of chronic dermatoses (psoriasis and atopic dermatitis) was analyzed in this study.
Primaderm (First group) was applied on the affected area one-two times a day up to clinical remission or in the period of 21 days. In the second (control) group of patients a traditional therapy including antihistaminic, sedative and hyposensitizing preparations was used.
The application of the preparation on the affected skin resulted in no complication. Just in some isolated instances the patients had a short-term feeling of an insignificant burning pain. There was not observed any damaging effect on the adjacent area.
The new preparation “Primaderm” for external application has anti-inflammatory, anti-pruritic, emollient action - affects reparative function of skin. Using of this preparation in complex with systemic antioxidants markedly improves therapeutic effect.
NONTRADITIONAL METHODS OF ANESTHESIA IN THERAPEUTIC STOMATOLOGY PRACTICE
J. Experimental and Clinical Medicine, 2005, 3, pp.45-48.
Stomatology Institute of Postdiploma Education, Tbilisi, Georgia
Using of anesthesia is stomatological practice, particularly electro acupuncture, is very effective in caries treatment. We used hte electro acupuncture apparatus “Acustim” for biologically active points. The method is especially acceptable for allergy-predisposed patients and pregnant women. It may be used not only as a basic course, but as an additional method also. The method is noninvasive, very simple and available in stomatology practice.
PARTIAL CYSTECTOMY AS ORGAN-PRESERVING TREATMENT FOR BLADDER CANCER
J. Experimental and Clinical Medicine, 2005, 3, pp.48-53.
Azerbaijan Medical University, Department of Urology, Baku, Azerbaijan
INTRODUCTION & OBJECTIVES. Radical cystectomy is a generally accepted method in the treatment of invasive bladder cancer (IBC). But taking into consideration the lack of an ideal method for urine derivation, deterioration of patients’ quality of life after the operation, preservation of bladder is the ideal result of IBC treatment. From this point of view partial cystectomy again acquires actuality in the treatment for IBC.
MATERIAL & METHODS. Between 1987 and 2005, 148 patients (pts) with muscle invasive bladder cancer were treated with partial cystectomy. Patients’ age ranged 21 to 82. Men were 118 (79, 7 %), women were 30 (20, 3 %). Men’s mean age – 58, 7 (range 28 – 82), women’s mean age – 53, 8 (range 21 – 77). Only partial cystectomy was performed in 94 pts, resection with a left-sided ureterocystoneostomy was in 26 pts; with a right-sided ureterocystoneostomy was in 22 ones, with a bilateral ureterocystoneostomy were in 6 ones. 27 pts were operated by means of the proposed method - transvesical resection of bladder tumor (TVRB) with ureteroneocystostomy (UNCS). TVRB with bilateral UNCS was fulfilled in 3 pts, in 24 pts with unilateral UNCS (in 13-on the left, in 11–on the right).
RESULTS. The postoperative observation period lasted 1 to 13 years (on the average 38 months). Tumor relapsed in 40 pts (27 %) after partial cystectomy. Early relapses (12 months) were observed in 21 pts (14, 2%), the 5-year survival rate - 72 %. Relapses after TVRB with UNCS - 22,2% (6 ones), the 5-year survival rate - 68 %. Analysis of the relapses development determined that 65 % relapsed tumors had been superficial, 35 % were invasive.
CONCLUSIONS. The analysis of the received results shows that partial cystectomy can be successfully used and can be an acceptable alternative to radical cystectomy in the treatment for muscle invasive bladder cancer in some selected patients.
14. N. KAKAURIDZE, I. KHAKHUTAISHVILI, G.ANDRIADZE
ERYTHROCYTES STRUCTURAL-FUNCTIONAL, OXIDATIVE AND ANTIOXIDATIVE PARAMETRES RELATIONSHIP WITH BLOOD LIPIDS IN PATIENTS WITH HEART FAILURE
J. Experimental and Clinical Medicine, 2005, 3, pp.53-58.
National Center of Therapy, Georgia
A.Natishvili Institute of Experimental Morphology Georgian AS, Tbilisi
The aim of the study is to establish the erythrocytes structural-functional, oxidative and antioxidative changes in development in patients with heart failure.
Materials and methods: There were investigated 52 patients: 22 women and 30 men (age 65,7±12,1). They were divided into three groups: I group- heart failure I-II class (n=28). II group - heart failure III-IV class (n=24), III control group – practically healthy persons (n=15).
The following parameters were studied: general blood analysis; serum lipids, total blood bilirubin, free (non-conjugated) bilirubin by “Human” reagents, malondialdehyde and phospholipids in erythrocytes and in plasma, erythrocytes morphological features were investigated by electron-microscopically method.
Data was processed statistically: M+SD (M-medium, SD-standard deviation) was calculated. Student¢ s t-test for match data was used in analysis of intergroup information. Reliability rate was determined as p<0.05. Correlation was tested according to Pearson correlation.
Results: There was statistically confident increase of erythrocyte malondialdehyde indexes in I, II comparison with III group (p<0.01), that indicate the increases of lipid peroxidation in patients with heart failure. The VLDL increased in I group significantly (p<0.001;) in comparison with III group and decreased in II group. On this background the increased level of free bilirubin is clear, as bilirubin is well known as antioxidant in lipid peroxidation cases.
Conclusion: In development of heart failure and oxidative stress free bilirubin increases, which has antioxidative quality, it can be considered one of the most important homeostatical links. Oxidative stress develops during the heart failure, which is proved by increased MDA in erythrocytes and plasma and by increased of erythrocytes agregability which were found in connection with hearth failure gravity stage.