1. PKHALADZE M., TAVZARASHVILI I., GORGOSHIDZE G., KURELI I., TSAGARELI M.
MORPHOLOGICAL CRITERIA OF GINGIVAL MUCOUS PATHOLOGY IN GINGIVITIS
J. Experimental and Clinical Medicine, 2006, 5, pp.9-12.
A.Natishvili Institute of Experimental Morphology, Georgian AS, Tbilisi, Georgia
As gingivitis – an inflammatory process is usually starting point of pathological changes in gingival mucous, the aim of our study was to assess the morphological changes and cellular content of the soft tissue in the model of experimental gingivitis.
We used sixty rats with body weight of 120-160 gr. in the experiment. Gingivitis was modeled by front teeth ligation after a preliminary exfoliation of mucouse membrane in the dental neck area. The specimens were examined by histological method and electron microscopy on the 3rd, 5th, 14th and 28th days of the experiment.
The investigation of the experimental gingivitis showed that fibroblasts, plasmocytes and collagenous fibrils have the main role in the pathogenesis of the pathological process and cause the changes in gingival.
RISK OF BRONCHIAL ASTHMA AND ACUTE OBSTRUCTIVE BRONCHITIS UNDER CHANGING CONTENT OF ZINC AND COPPER IN BLOOD SERUM AND LYMPHOCYTES
J. Experimental and Clinical Medicine, 2006, 5, pp.9-14.
State Research Institute of Pediatry, Georgia, Tbilisi
The purpose of the research: Estimate the risk of bronchial asthma and acute obstructive bronchitis during zinc and copper content changing in the blood serum and lymphocytes.
We have studied the content of microelements - zinc and copper – in the blood serum and lymphocytes of 127 children aged 6-15 years with persistent bronchial asthma (PBA) during the attack, average and grave forms of PBA and acute obstructive bronchitis (AOB), and assessed the risk of disease development.
Identification of the diagnosis was carried out by the GINA standard. Definition of a level of zinc and copper in the blood serum and lymphocytes was performed by the spectrometric method. Mathematical support was accomplished with the help of the package of statistical programs SPSS 11-5.
The results of the research showed that the relative risk of AOB increases during the elevation of zinc content in the blood serum (RR=4,97; 95%CI:2,55-9,70); the relative risk in the grave form of PBA increases during zinc content decrease in the blood serum (RR=2,34; 95%CI: 1,43 - 3,84) and lymphocytes (RR=2,50; 95%CI: 1,51 -4,14); the relative risk of all disease increases when the copper content lowers in the serum during AOB (RR=7,09; 95%CI: 2,82- 17,78); in IBA (RR=1,63; 95%CI: 1,10- 2,42). In the average form of PBA (RR=2,47; 95%CI: 1,55- 3,94); in the grave form of PBA (RR=2,68; 95%CI: 1,59- 4,50).
3. KHAKHUTAISHVILI I., JANGAVADZE M., SHAISHMELASHVILI G.
MORPHO-FUNCTIONAL PARAMETERS OF ERYTHROCYTE MEMBRANE AS IHD PROGRESSION MARKER
J. Experimental and Clinical Medicine, 2006, 5, pp.15-17.
A.Natishvili Institute of Experimental Morphology of Georgian Academy of Sciences
The purpose of the research is to establishe morpho-functional parameters of the erythrocyte membrane in the diagnosis of IHD aggravation. Methods: 80 patients have been investigated (age 65±4). which have divided into 3 groups aggravated IHD, plus one control group (actually healthy people). We studied malondialdehyde (MDA) and phospholipids (PhL) in erythrocyte membranes and in blood plasma, and also erythrocyte membrane-fragility. Results: the MDA rate has been increased in the IHD group in comparison with the control group. Also there was a substantial growth of MDA level within the limits of the IHD groups. These changes were most significant in the erythrocyte membrane than in the plasma. On the contrary, the rate of phospholipids has been reduced. We have also found that osmotic fragility was decreased on the IHD stages. Conclusion: Our study has shown that quantity of MDA and PhL in the erythrocyte membrane strongly correlates with IHD severity. Therefore, these parameters can be used as clinical markers for IHD progression.
4. JANDIERI L., KIKALISHVILI L.
INFLUENCE OF TRUNCAL VAGOTOMY ON HEPATOCYTE MONOOXYGENASE SYSTEM
J. Experimental and Clinical Medicine, 2006, 5, pp.17-20.
TSMU Department of Topographic Anatomy, Tbilisi, Georgia
Experiments were performed on 25 white rats (with 180-250 g body weight). The rats were distributed in 2 - control (5 animals) and experimental (20 animals) groups. The truncal subdiaphragmal vagotomy was performed.
The activity of microsomal proteins, cytochrome P-450 and b5, NADPH-cytochrome p-reductase, p-anilinhydroxylase and amidopirin-N demetilase was studied in the microsomal fractions of the hepatocytes in the intact animals, on the 3-rd, 10-th, 20-th and 30-th day after operation.
It was shown that the rate of the restoration of the liver monooxygenase system, activity correlates with postoperative period duration.
5. K.KHUTSISHVILI, R.MAGALASHVILI, L.TOKHADZE, Z.DEMETRASHVILI, L.GOPODZE
HUMAN SS ANTIGEN HLA AND ABO SYSTEMS IN THE PROCESS OF OPERATIVE WOUND HEALING
J. Experimental and Clinical Medicine, 2006, 5, pp.20-21.
Department of Surgery ¹ 4, Tbilisi State Medical University, Georgia
Association of the HLA and ABO antigen systems with wound healing process was investigated on 68 Georgian patients. Based on the peculiarity of the wound process course, two groups of the patients have been distinguished. The first group united 34 patients with primary intention of the operative wound, while the second group united 34 patients with complicated course of the wound healing process. Our study showed that the distribution of the HLA and ABO systems antigens is different in these groups. Genetically determined risk of wound complication development was significantly increased in the individuals with B(III) group of blood and is associated with HLA-DR1, the relative risk (RR) 4,29.
6. KASRASHVILI M., PAILODZE M.
EFFECTIVENESS OF RADIOPROTECTIVE ACTION OF VITAMIN C
J. Experimental and Clinical Medicine, 2006, 5, pp.22-24.
Institute of Medical Biotechnology, Georgian Academy of Sciences
The aim of the study was to investigate the effectiveness of vitamin C radioprotective action on the rat ovary during γ-radiation.
According to the results of the investigations, it was established that during irradiation the mechanisms of folliculogenesis, ovulation and luteinization are disordered in the rat ovary. Hyperplasia of hormonproducing structures was revealed by means of tecamatosis. During postirradiation action of vitamin C in the ovary with disordered folliculogenesis, in a single follicle the oocyte structure is preserved, though in dystrophic conditions. Hyperemia was not revealed in the stroma. It may be concluded that vitamin C contributes to the activation of folliculogenesis, stroma hyperplasia and tecamatosis, inhibition of the hyperplasia of hormonproducing structures.. Preventive action of vitamin C for 5 days before radiation provides maintenance of a normal structure of oocytes in the follicle and increased hyperplasia of the stroma. There wasn’t revealed any tendency of tecamatosis.
7. GRIGOLIA N., NANEISHVILI M., LOMTADZE R., CHKHAIDZE G.
MULTIPLE HYDATID CYSTS OF ABDOMINAL CAVITY
J. Experimental and Clinical Medicine, 2006, 5, pp.24-25.
Department of Surgery N2, Tbilisi State Medical University;
Central Railway Hospital, Tbilisi, Georgia
5 cases of multiple hydatid cysts of the abdominal cavity were analyzed. In 4 cases multiple cysts had been developed after traumatic rupture or after dissemination during the operation (secondary spread). Primary spread –hematogenous dissemination occurred in 1 patient. All the 5 patients were operated on. Postoperative antihelmit therapy is indicated in all cases.
8. DOGONADZE S., GORDEZIANI M., SANIKIDZE T., NINUA N.
VARIATION OF OXYDATION METABOLISM IN VIRAL POLYNEUROPHATIES
J. Experimental and Clinical Medicine, 2006, 5, pp.25-27.
Neurology Department of Tbilisi State Medical University, Georgia
The pathogenesis of viral polyneurophaties is being too much discussed, as several metabolic and vascular patholigical processes act to each other. The aim of our study was to define determinants of oxidation process and NO content in the blood of patients with viral polyneurophaties. On summarising our results showed that in viral polyneurophaties free-radical processes are activated and at the same time the antioxydant reserves of fermental systems are exhausted, resulting in NO supression in the blood of patient with viral polyneurophaties. NO dismetabolism is a cause of the damage to cell membrans and nerves.
NERVOUSNESS IN HEALTHY AND OFTEN CHILDREN IN CORRELATION WITH SOCIAL FACTORS
J. Experimental and Clinical Medicine, 2006, 5, pp.28-31.
Children’s XX Polyclinic, Tbilisi, Georgia
The goal of the research is to determine the level of nervousness in healthy and often children in correlation with social factors. 120 healthy and 28 often children were tested. They were devided into two groups according to the age: 60 healthy children aged 4-6, 60 healthy children aged 7-12, 8 often children aged 406, 20 often children aged 7-12. The tests were done in the period of remission.
The same tests were done in 1-2 years period. The level of nervousness was studied according to the methods of Imen and Dork, and in children aged 7-12 the level of nervousness was studied by standardized test os Sasacin. The dependence between diseases and social factors was not noticed (p>0,289), but the correlation between healthy children and social factor and the dependance between them was statistically quite trustful p=0,001.
Social factors were studied according to the individual cards.
10. DEDABRISHVILI A., REKHVIASHVILI V., TEVZADZE M., APRIDONIDZE K., SILAGADZE J., KORSANTIA B.
IMMUNE STATUS IN WOMEN SUFFERING FROM CANDIDIASIS VULVOVAGINITIS
J. Experimental and Clinical Medicine, 2006, 5, pp.31-33.
Tbilisi State Medical University,
K.Chachava Institute of Obstetrics, Gynecology and Perinatalogy,
Institute of Medical Biotechnology, Tbilisi, Georgia
Consideration of the obtained results confirm that during chronic vulvovaginitis we deal with acut sharp immune deficit of nonspecific immune factors, which is expressed in the decrease of the phagocyte index by 50%, decrease of total number of phagocytes by 20%, decrease of α-interferon by 25% and decrease of γ -interferon by 50%. Cell and humoral parameters suffer insignificant changes.
Proceeding from the above stated, to get the anti-fungal stable therapeutic effect and to extirpate the chronic urogenital candidiasis relapses, we recommend to include immune correcting preparations in the therapeutic complex measures, thus, for example, phagocyte activators, interferon and interferon genes. They stimulate leukocyte group cells and increase number of phagocyte neutrophiles.
11. KHACHKACHISHVILI M., MANJAVIDZE N, GUDJABIDZE R.
INFLUENCE OF VARIOUS TYPES OF ARTIFICIA FEEDING ON THE MORBIDITY OF INFANTS
J. Experimental and Clinical Medicine, 2006, 5, pp.33-38.
Tbilisi State Medical University;
Brothers Zubalashvili Pediatrics Clinic, Tbilisi, Georgia
We observed a cohort of 96 newborns aged to 6 months out of the Children’s Polyclinic N10 and Brothers Zubalashvili Pediatric Clinic in Tbilisi. All the children, according to the type of feeding were divided into three groups: group 1 (a control group) – 33 infants fed by sweet adapted artificial formula; group 2 - 33 infants fed by sour adapted artificial fomula; group 3 – 30 babies fed by lactoacid national product “Mariami” – 30. We defined a decrease in the absolute risk and relative risk of morbidity, and the number of the patients necessary to treat for gaining one positive result after introducing the sour products and lactoacid product “Mariami” in the feeding scheme. We defined the differences between the groups by criterium χ2 (Pearson). Mathematical software was provided by means of the pack of statistical programs SPSS 11-5.
The results gained through the study show significant decrease in the absolute and relative risks of the atopic dermatitis RR = 0,10 (95%CI: 0,03- 0,41) ARR = 0,57 (95%CI: 0,38- 0,76); decrease in the absolute and relative risks of rachitis RR = 0,28 (95%CI: 0,09- 0,88); ARR = 0,264 (95%CI: 0,07-0,4); decrease in the absolute and relative risks of dyspepsia RR = 0,14 (95%CI: 0,03- 0,55); ARR = 0,418 (95%CI: 0,23- 0,61); NNT=2 (95%CI: 0,38- 0,76).
Thus, the application of the natural lactic acid product – “MARIAMI” – has advantage in relation to sweet adapted food mixtures. Especially high indicators may be reached by feeding a baby by a national lactoacid product “Mariami” what indicates its high nutritive value, good assimilation of the product and gives the basis to recommend “Mariami” to be used on a wider scale.
12. MARTIASHVILI Z., AZIKURI G., ZANANIAN I., KINTRAIA P.
CHANGES IN LOCAL CORTICAL BLOOD FLOW OF NEONATAL RATS DURING ISCHEMIC PRECONDITIONING AND INHIBITION OF NITRIC OXIDE SYNTHASES
J. Experimental and Clinical Medicine, 2006, 5, pp.38-41.
K.Chachava Research Institute of Perinatal Medicine and Obstetrics-Gynecology, Tbilisi;
Beritashvili Institute of Physiology, Tbilisi, Georgia
The aim of this study was examination of the Nitric Oxide role in realization of ischemic preconditioning phenomenon. Experiments were carried out on white rats. Ischemic preconditioning was accomplished by occlusion of the common carotid artery. Cerebrocortical blood flow was measured by Hydrogen clearance technique. Intraperitoneal injection of Nitro-L-arginine-metil ester (L-NAME) resulted in complete suppression of preconditioning, while selective inhibitor of inducible NOS – aminoguanidin suppessed it just partially.
THE RAT MUSCULUS GASTROCNEMIUS MORPHOLOGY AT VARIOUS AGE PERIODS IN DIFFERENT REGIMES OF PHYSICAL LOAD (RAPPORTE 1)
J. Experimental and Clinical Medicine, 2006, 5, pp.41-43.
Azerbaijan State Medical University, Baku
Objectives. 120 white male rats at 1) young, 2) preadolescent, 3) mature, 4) senile age period (30 animals in each group) in condition of forced swimming. The musculus gastrocnemius after 1) single maximum load, 2) 3-5 months after a moderate load is studied by the histoenzymochemical and electronmicroscopic methods of analysis.
Materials and methods. Activity of oxydoreductase enzymes: SDHLDH and NAD diaphorases and ultrastructure were determined specimens of m. gastrocnemius in different regimes of physical load. Glycogen content also is shown by PAS-reaction.
Results. Activity of LDH and NADH diaphorase are significantly increased in muscle fibers, endema of sarcoplasm and various variants of mitochondrial damages are shown after maximum physical load in both groups of animals. There is a parallelism between the content of histochemically marked activity of red muscle fibers and rats age, howerer SDH is more active in the mature age rats group after moderate regime of load, while in the others SDH activity response and glycogen content are reduced. Thus, a single maximum tolerable physical load in animals of all the age groups induces the processes of cells and tissues hipoxic disturbance. A moderate load is realized as a trained effect, particularly in mature and preadolescent animals.
DETERMINATION OF INTER-GROUP BONDS OF PSYCHODYNAMIC INDICATORS AMONG GEORGIAN WOMEN OF DIFFERENT AGES
J. Experimental and Clinical Medicine, 2006, 5, pp.43-47.
TSMU, Chair of Normal Human Anatomy, Tbilisi, Georgia
The aim of the research was determination of inter-group bonds of psychodynamic indicators of researching contingent individuals of different the scales of age. For this purpose we have studied 20 - 55 years old 175 Georgian women, which were grouped in 7 groups with 5-year interval. Personal indicators (character, temperament, intellect, mood) of each individual a was determined. It was established that correlations in the limits of 0.2-0.3 are noticed in all forms of character and temperament among 20-24 and 25-29 years old women; correlations among melancholic temperament and all intellect subtypes are in the limits of 0.2-0.3; among phlegmatics – more than 0.3; among cholerics the – 0.2-0.3; and among sanguine – more than 0.3.
Between temperament and mood subtypes there are the following inter-group bonds: melancholic and phlegmatic temperament with excitement – more than 0.2 or 0.3; choleric – 0.1-0.2; and between sanguine temperament and types of mood the correlations are in the limits of 0.3.
Scale of age of 45-49 years old women – inter-group bonds are high among phlegmatic temperament and both types of the mood – more than 0.3; the relation between phlegmatic temperament and logic intellect is also high – more than 0.4; to verbal intellect – more than 0.5; the relation with each type of the mood is higher than 0.3.
Scale of age of 50-54 years old women – correlations in this group are high among phlegmatic temperament and both types of the mood – in the limits of 0.4; the inter-group relations between phlegmatic temperament and all types of intellect are also high – more than 0.4; these relations are also in the limits of 0.4 between phlegmatic temperament and each type of the mood.
Thus, by the research it was established that young Georgian women are extroverts, i.e. stabile, lively, with sanguine temperature, i.e. strong, active, patient, balanced, having sharply expressed middle logic intellect, plastic and dynamic, constant and stabile, constitutional types with middle excitement; while elders and comparatively aged women have phlegmatic temperament, i.e. they are passive, cautious, thoughtful, abstinent, liable and calm, inert, and balanced. According to character they are introverts, i.e. directed to the inner, to the subject, less communicable; they are constitutional types having middle verbal and middle logic intellect, conflict and static mood; though there are mixed forms as well.
15.TKESHELASHVILI B., JUGELI M.
EXPERIENCE OF USE OF COLPOSCOPY USE IN PRIMARY DIAGNOSIS OF SEXUALLY TRANSMITTED DISEASES IN WOMEN
J. Experimental and Clinical Medicine, 2006, 5, pp.48-50.
«David Tatishvili Medical Centre» Ltd, Tbilisi, Georgia
Frequency of Sexually Transmitted Diseases (STD) still remains stably high in the whole world. The aim of our investigation was scientific evaluation of diagnostic significance of colposcopic signs in patients with cervical chlamydia-mycoplasmatic infections (CMGI). Material and Methods. Colposcopic examination was performed in 952 women (age - from 16 to 67). The I group unified 805 patients with colposcopic signs of CMGI, the II group – 147 patients without colposcopic signs of CMGI. Specific colposcopic features of chlamydia-mycoplastic genital infections – lymphatic follicles and “vessel starlets” were revealed, which were compared with results obtained from “gold standard” methods – direct immunofluorescence assay(DIA) of cervical smears. Results. In I group in 725 cases lymphatic follicles have been revealed (90%), in 80 cases (10%) – “vessel starlets”. In 29 cases colposcopy data was not confirmed by DIA. In II group CMGI were diagnosed in 36 patients (24,5%). High specificity (0,79), sensitivity (0,96) and diagnostic effectiveness (0,93) of colposcopy were confirmed. Conclusion. Our findings suggest application of colposcopy as initial method for examination patients with STD, optimization of diagnostic process and decrease of economic costs.
16. BUCHUKURI I., KITUASHVIL T.
QUALITY OF LIFE OF CHILDREN WITH ATOPIC DERMATITIS AND THEIR FAMILIES
J. Experimental and Clinical Medicine, 2006, 5, pp.50-53.
Research Institute of Dermatology and Venereology, Georgia
The goal of the research was to study quality of life of the diseased children, impact of the disease on their families, and to establish correlation between the severity of the clinical course of atopic dermatitis according to the SCORAD-index and quality of life, and also quantitative parameters giving full assessment of the disease course in the bio-socio-psychological aspect.
The severity of the disease course was assessed by the SCORAD-index; quality of life in the first group was studied according to the questionnaire Infants Dermatology Life Quality Index (IDLQ), in the second group – the Dermatology Life Quality Index questionnaire. The assessment of life quality in this group was performed not only by the parents but by the patients themselves. In both groups was studied the influence of the disease course on the family according to the questionnaire Atopic Dermatitis Family Impact Scale (FDFIS). The study showed that severity degree of the course of atopic dermatitis strongly impacts life quality of patients and their families. The influence is especially prominent in the 4-18 years old age group and during grave course of the disease, whereas atopic dermatitis with light course in the 4 years younger age group slightly impacts life quality. The most serious factors of the disease influence on a family were insomnia of a diseased child parents, financial problems, and also difficulties with feeding and washing.
17. MAKARADZE D., PESHKOV T., TSISKARIDZE A., JANELIDZE M., SHAKARISHVILI R.
STRUCTURE OF STROKE-ASSOCIATED COMORBID PATHOLOGY
J. Experimental and Clinical Medicine, 2006, 5, pp.53-57.
P.Sarajishvili Institute of Neurology, Tbilisi
Batumi Republican Clinical Hospital, Georgia
Cerebral stroke as a polyetiologic disease is characterized by the comorbid pathology combined with its complex structure. Its study is of great importance in stroke prevention, diagnosis and treatment.
The goal of the research was to study the structure of comorbid pathology combined with stroke in accordance with Batumi stroke register from 1/09-98 to 1/10-200.
Study methods: all the hospital and nonhospital cases of cerebral stroke in Batumi have been studied by the prospective clinico-epidemiologic method. A standard protocol was filled in for each patient, listing anamnestic and confirmed clinico-paraclinical comorbid diseases.
Results: there was revealed a high correlation of arterial hypertension with all types of stroke. The frequency of the other comorbid diseases reflected their significance as risk factors with respect to a concrete type of stroke.
Besides, there was revealed rather a high index of liver pathology comorbidity regarding hemorrhagic, as well as ischemic stroke, whereas the infectious pathology was encountered 3 times more frequently under hemorrhagic stroke than in the ischemic one.
18. GLONTI L. SAMUSHIA O. MANJGALADZE C. MAKHARADZE T.
SOME ASPECTS OF CEREBRAL ISHEMIC STROKE TREATMENT
J. Experimental and Clinical Medicine, 2006, 5, pp.57-59.
Neurology Department of Tbilisi State Medical University, Georgia
During the severe duration of ischemic stroke we applied the test of blood dilution by saline and non-saline solutions to reveal the individual reactions of homeostasis on the dilution. We analyzed 102 cases of ischemic stroke with the age ranging 41-78. The received data we used for the infusion therapy management, prevention of thromboembolic complications and the prognosis of the duration of the illness.
19. SUPATASHVILI t., GABUNIA U.
TYPES OF THE BREAST CANCER PARENCHIMAL CELLS AND THERE ULTRASTRUCTURAL characterization
J. Experimental and Clinical Medicine, 2006, 5, pp.59-61.
A.Natishvili Institute of Experimental Morphology, Georgian AS, Tbilisi, Georgia
In the breast cancer there are a 5 subtype of the parenchimal cells, differs by there ultrastructural features.
I cell type –ultrastructural features is similar with the normal epithelium of the breast (secretor granules, intracellular channels, myoepithelial cells) and organelle rich cytoplasm.
II cell type – They contained the same features and components but in less amount. Organelle pure cytoplasm.
III cell type – presented with myoepithelial cells.
IV and V cell types – They do not have normal breast epithelial features, but cytoplasm is rich (IV type) or pure (V type) with organelles.
All cell types my be electron dense or not.
I, II, and III type of cells are common for more differentiated forms of the breast cancer (tubular, papillar, comedo) and IV and V cell type – for less or not differentiated forms.
20. KVIRIKASHVILI M., SUJASHVILI L., SANIKIDZE T., METREVELI D.
CORRECTION OF OXIDATIVE METABOLISM IN MYOCARDIUM AT EXPERIMENTAL THYROTOXICOSIS BY OLIGOCRINE-T
J. Experimental and Clinical Medicine, 2006, 5, pp.62-64.
Medical Academy of Tbilisi;
Tbilisi State Medical University, Georgia
There are numerous studies that evidence significant role of oxidative stress in the pathogenesis of thyrotoxicosis. Hence, great importance has been attached to the application of antioxidants with the purpose of prevention of hypothyroidism-induced toxicosis. The object of our study is evaluation of oxidative metabolism alterations in myocardium and assessment of protective efficacy of oligocryne in experimental thyrotoxicosis. Based on the analysis of obtained results we can conclude that intensity of peroxidative processes at thyrotoxicosis correlates with the degree of myocardium damage (extent of histomorphological alterations). Antioxidant preparation, Oligocrine facilitates a decrease in the intensity of peroxidative processes in body that results in mitigation of oxidative damage of myocardium and provides maintenance of cardiomyocyte structure.
21. GAMEZARDASHVILI T., KIPSHIDZE N., TALAKVADZE T.
THE ROLE OF TUMOR NECROSIS FACTOR-a IN THE DEVELOPMENT OF INSULIN RESISTANCE AND OTHER DISORDERS IN METABOLIC SYNDROME
J. Experimental and Clinical Medicine, 2006, 5, pp.64-68.
National Center of Therapy, Tbilisi, Georgia
Aims: Insulin resistance and hyperglycemia are very frequent disorders, they are describe as the dominant risk factors for cardiovascular disease. There are few studies in which relationships between insulin resistance, TNF-a and ferritin have been studied.
Methods and Results: Plasma levels of insulin, glucose, total protein, albumin, creatinine, ferritin, iron, lipid and inflammatory markers and TNF-a were assessed in 70 metabolic syndrome patients.
The statistical analysis showed correlations between metabolic parameters and parameters related to insulin resistance.
Conclusion: Our findings support the hypothesis that chronic inflammation is a common antecedent of the metabolic syndrome and has been related of insulin resistance and abdominal obesity.
This study has shown that increased TNF–α concentration is assotiated with decreased albumin level in patients with metabolic syndrome.
COURSE OF POSTPARTUM PERIOD AMONG WOMEN WITHIN BABY FRIENDLY HOSPITAL INITIATIVE
J. Experimental and Clinical Medicine, 2006, 5, pp.69-72.
Department of Obstetrics and Gynecology N2, Medical Academy of State Medical University, Tbilisi, Georgia
Baby Friendly Initiative practices (skin to skin contact, breastfeeding on demand and etc.), recommended by WHO, is one of the main strategy on Safe Motherhood Initiative. Postpartum period of 200 women delivered in teaching hospital before and after implementation of Baby Friendly Hospital Initiative were reviewed and information was analyzed .There was an important statistically significant improvement in postpartum period (blood loss, involution of uterus, confirmed by ultrasound), in group of women treated according to principles of Baby Friendly Hospital Initiative.
The implementation of the Baby Friendly Hospital Initiative in maternity units would contribute towards an increase of exclusive breastfeeding of newborns and also significantly improve the process of adaptation of newborns, therefore this strategy should be widely disseminated in every maternity unit.
23. MIMINOSHVILI M., CHKUASELI G.., ZURABISHVILI Z.
morphological features of the uterine leiomyoma nodes and intact myometrium
J. Experimental and Clinical Medicine, 2006, 5, pp.72-75.
Department of Fundamental Medicine of State Medical Academy, Tbilisi, Georgia
Background. Uterine leiomyoma is the most common solid tumor in adult women affecting at least 30% during their reproductive years. Despite its prevalence, little attention has been directed toward the causation and pathogenesis of this disorder. Uterine fibroids rank as the major reason for hysterectomy in the world, accounting for approximately one-third of all hysterectomies.
Materials and Methods. Humane myometrial and leiomyoma tissue was obtained from 20 women after hysterectomy. Tissue samples were investigated by light and electron microscopy.
Results. Microscopically, the tumors were formed by interlacing bundles of spindle cells arranged in a whorl-like pattern. In the leiomyoma tissue myocytes, there were a some ultrastructural changes, which resemble to the proliferative-secretory type of SMC and also to the ischemic injury.
Conclusion. Based on our findings, we can conclude that endothelial dysfunction can play a pivot role in the pathogenesis of leiomyoma. Functional inactivity and insufficient transendotelial transport activity lead to the tissue ischemia, which is a promoter of proliferation and transformation of the myocytes.
ANTIULCER ACTION OF 900 AND 743 COMBINATION ON ETHANOL-INDUCED GASTRIC ULCER IN RATS
J. Experimental and Clinical Medicine, 2006, 5, pp.75-77.
A.Natishvili Institute of Experimental Morphology, Georgian AS, Tbilisi
Earlier we investigated the antiulcer effects of sydnoneimine derivatives OF 743 and OF 900 in rats using experimental model of ethanol and stress, induced ulceration of Gastric mucus. It was shown that both of them have protective antiulcer effect. In this study we investigated the combination of these two agents in the following doses: 0.02+0.02 – 20+20mg/kg. The combination of OF 743+OF 900 has a dose dependent effect and reduces the incidence of gaster mucous damages by 51% - 85%.
25. VIRSALADZE D., JAVASHVILI L., ESAKIA N., ESAKIA A., DEMETRASHVILI L.
DYNAMICS OF METABOLIC CHARACTERISTICS AND COAGULATION PARAMETERS IN PATIENTS WITH METABOLIC SYNDROME DURING METFORMIN THERAPY
J. Experimental and Clinical Medicine, 2006, 5, pp.78-80.
Department of Endocrinology & Metabolism, Tbilisi State Medical University, Georgia
The aim of our investigation was to study the dynamics of metabolic characteristics and coagulation parameters in patients with metabolic syndrome (MS) 3 and 6 moths after metphormin(Siofor Berlin-Chemie) therapy initiation. 30 MS patients have been investigated. MS was assessed by WHO definition. All metabolic and haemostatic parameters there measured. The diet and metphormin (Siofor mean dose – 1000 mg/day) have been prescribed to all patients. The number of MS patients after a 3 - month therapy was significantly decreased from baseline one (p0-3=0.005). The same tendency was observed in the case of 6 month therapy in comparison of both baseline (p0-6<0.001) and 3 month values (p3-6<0.001). Metphormin therapy during 6 months sharply improved insulin sensitivity (p0-6=0.020) and, respectively, decreased insulin resistance (p0-3=0.003, p0-6=0.001). The 3 and 6 month therapy showed significant dynamics of almost all metabolic and coagulation parameters. In conclusion, metphormin therapy have positive influence both on the MS characteristics and on the coagulation parameters. The obtained results suggested that hypercoagulation might be included in the MS features.
IMMUNOHISTOCHEMICAL CHARACTERISTICS OF ACTINIC KERATOSIS AND TUMORS OF THE SKIN
J. Experimental and Clinical Medicine, 2006, 5, pp.84-89.
Tbilisi State Medical University, Department of Pathological Anathomy, Georgia
The aim of our study was to elucidate the immunohistochemical characteristics of actinic keratosis and skin tumors. We examined immunocompetent patients with premalignant lesions of the skin such as actinic keratosis and tumors such as basal cell and squamous carcinoma. 10 patients were involved in each group. The clinical study included gross features of the lesion, growth rate, colour, size. Paraffin sections from biopsy specimens were stained by hematoxylin-eosin and van Gieson. Immunohistochemistry was performed using monoclonal antibodies against HPV, oncoprotein p53, anti-apoptotic protein Bcl-2, proliferation marker PCNA. Strongly, moderately and weakly positive cells were counted. Actinic keratosis and skin tumors failed to show specific clinical features, therefore they can not be diagnosed based on the clinical signs only and morphological examination seems to be mandatory. The immunohistochemical study has showed that HPV was positive in 60% of actinic keratosis and in 40% of basall cell and squamous carcinoma. Our results suggest that HPV+/p53+ types of actinic keratosis are characterized by a higher proliferation activity in comparison to HPV-/p53+ types, and expression of Bcl-2 is associated with HPV-negativity. Therefore this premalignant lesion of the skin requires immunohistochemical examination with evaluation of the expressions of human papillomavirus, proliferation marker PCNA and anti-apoptotic protein Bcl-2. The differential diagnosis of actinic keratosis and skin tumors (basal cell and squamous carcinoma) should be based on the following immunohistochemical criteria: proliferation activity is higher in HPV+ types of actinic keratosis than in HPV- types (p<0,05), but does not differ between HPV+ and HPV- types of tumors. In HPV+ and HPV- types of basal cell and squamous carcinoma the incidences of p53-positivity and proliferation activity are similar. However, the strong expression of both markers (p53, PCNA) is related to the lower grade of squamous carcinoma. Therefore, in order to differentiate basal cell and squamous carcinomas, grading of squamous carcinoma should be done first based on the histologic picture, and expression of immunohistochemical markers should be assessed according to the grade.