EXPERIMENTAL & CLINICAL MEDICINE
2007, ¹ 6
1. GOGNADZE T., MUKHADZE I., DJINCHARADZE D., TSINTSABADZE N.
QUANTUM THERAPY IN PATIENTS WITH UROGENITAL CLAMYDIOSIS
J. Experimental and Clinical Medicine, 2007, 6, pp. 14-17.
Tbilisi State Medical University, Georgia
We have analyzed a clinical-laboratory study of the patients at risk of abortion caused by chlamydia infection in the I trimester of pregnancy. 52 women were studied for chlamydia infection. They were divided into two groups. Group I (control) was comprised of 12 possible abortion patients with chlamydia infection who were treated by traditional therapy. 40 patients in the II – basic group were treated by traditional therapy plus photon therapy (apparatus of Father). Clinical trial was designed as a randomized distributed group (Method of Convert). This new treatment-prevention method is proven and available, and gives us the opportunity to increase the treatment efficacy.
2. L.GABUNIA, N.KURIDZE, L.RATIANI, SH.PETRIASHVILI, B.ARZIANI
PHARMACOLOGICAL CORRECTION OF FUNCTIONAL PARAMETERS OF STRESSED RAT ISOLATED HEART WORKING UNDER ISCHEMIA-REPERFUSION CONDITIONS
J. Experimental and Clinical Medicine, 2007, 6, pp. 17-18.
Tbilisi State Medical University, Georgia
It’s known that prevention and treatment of acute conditions related to myocardial ischemia and reperfusion still represent the actual problem in modern medicine. The goal of our work was to estimate the effect of Carvedilol – β-adrenoblocker of the III generation with an additional vasodilatative effect – on working (heart beat frequency, coronary stream rate (ml/sec) stressed rat heart isolated by the Langendorf method during ischemia and reperfusion.
Modification of low-stream global ischemia followed by reperfusion took place after heart beat stabilization. There were registered: the beating frequency in intact heart, coronary stream rate, and total quantity of arrhythmia under ischemia and reperfusion.
According to our research, Carvedilol protected the myocardium from ischemia and reperfusion, which is the additionally confirms the cardioprotective (antioxidative) and anti-ischemic action of Carvedilol.
3. RATIANI L., PETRIASHVILI E., KARCHAVA G., JOJUA M., MUSELIANI T., CHACHUA M., NIKOLAISHVILI M.
ANTHROPOMETRIC CHARACTERISTICS OF GEORGIAN PUPILS’ NUTRITION
J. Experimental and Clinical Medicine, 2007, 6, pp. 18-20.
Scientific Center of Radiobiology and Radiation Ecology;
Sokhumi State University, Faculty of Exact and Natural Sciences, Tbilisi, Georgia
In the anthropogenic characteristics (body weight/height, height/age, weight/age) of children’s nutrition there are indicated negative changes. Among different anthropogenic indexes nutritional disorders mostly are expressed by the index - body weight/height, where 2 standard changes have been recorded in 15.9% of children. Delay in physical development is mostly expressed in 6-7 years old children. Of course, the material of the anthropogenic study is rather small to let us make representative conclusions, but it must be noted that food supply for children needs appropriate improvement.
HISTO/CYTOPATHOLOGY OF THE THYROID GLAND
J. Experimental and Clinical Medicine, 2007, 6, pp. 20-34.
K. Eristavi National Center of Experimental and Clinical Surgery;
Diagnostic Cytology and Histology Laboratories, Tbilisi, Georgia
In the article ”Intra- and postoperative morphological diagnosis of thyroid gland pathology” published in the journal ”Experimental and clinical medicine” 2005, #14(23), Zhghenti C. and Dvaladze N. offered intraoperative cytological diagnosis of thyroid gland pathology with a further postoperative cytologic and histologic verification as a method for determining an adequate volume of surgucal extirpation. The given work presents the illustrations of the aforementioned article. The photograps have been taken by means of Pentium 4, using spacial camera Vimicro USB PC Camera (ZC0301PL) and program “Image Analyse”, microscope “Carl Zeiss Jena”, Germany, magnification x 5,10, 5mkm thick sections, fixation in 10% neutral formalin, embedding in celloidin, staining with hematoxylin and eosin; smears fixed in 96% spirit and stained in the Gimza-Romanovsky’s concentrated solution.
The illustrations depict forms of struma and strumitis during hypo- and hyperthyreosis, natural correction of these states, and benign and malignant tumorous progression.
PROGNOSTICATION OF TRANSFORMATION OF LOCAL INFECTIOUS-INFLAMMATORY DISEASES (LIID) INTO SEPSIS IN INFANTS
J. Experimental and Clinical Medicine, 2007, 6, pp. 34-38.
Azerbaijan Medical University, I Department of Pediatric Diseases, Baku
For substantiation of prognostic criteria, A.Valda’s consecutive statistical analysis has been used. Thus, patients’ individual immune-pathological parameters have been studied and a prognostic level has been specified. Dynamic supervision has shown that the top and bottom levels of clinic-immune-pathological parameters can be used as a criterion of LIID transformation into sepsis.
Prognostic level +13 and above specifies an unfavorable course of LIID and their transformation into sepsis. The overall prognostic level within the limits of +13-13 is considered uncertain, -13 and below - specifies a favorable course of illness.
6. PANTSULAIA I., KOBYLIANSKY E.
TISSUE INHIBITORS OF METALLOPROTEINASES, TRANSFORMING GROWTH
FACTOR-BETA1, AND OSTEOARTHRITIC CHARACTERISTICS
J. Experimental and Clinical Medicine, 2007, 6, pp. 38-41.
Sackler Faculty of Medicine, Tel Aviv University, Israel;
Institute of Medical Biotechnology, Tbilisi, Georgia
The debilitating destruction of joint tissues seen in osteoarthritis (OA) is due in large part to the degradative activity of metalloproteinase (MP) enzymes that target extracellular matrix (ECM) components within articular cartilage. Although successful in suppressing the pain and inflammation associated with this disease, conventional OA therapeutics does not inhibit the underlying tissue catabolism, allowing the disease to progress into irreversible ECM loss and chronic disability. In view of this situation, there is growing interest to specify biochemical markers that would indicate the onset of this disease. Tissue inhibitors of metalloproteinase, namely TIMP-1 and TIMP-2, have been implicated in cartilage turnover, and in cases of metalloproteinase imbalance are assumed to play an important role in the connective tissue destruction associated with idiopathic osteoarthritis (OA).
In the present research, we attempted to clarify the phenotypic relationship between TIMP-1, TIMP-2, TGF-β1, and hand OA in an ethnically homogeneous sample of families. Preliminary analysis of our data showed very little correlation between TIMPs and hand OA, and mostly non-significant in the total sample. However, in the group of affected individuals (e.g. those who had K-L score > 2 at any of the examined joints) the corresponding correlation was higher, namely, for OA (r=0.386, P< 0.01). Furthermore, we found that the TGF-β1 plasma levels and OA were associated significantly (P£0.05) both in the male and female cohorts (r=0.29 and 0.27, respectively). In conclusion, this study suggests that plasma level of TGF-β1 might be a surrogate marker of detecting OA at an early stage and give additional information to clinical and radiological findings with regard to the severity of the diseasse.
7. GOGNADZE T., MUKHADZE I., JINCHARADZE D., DOLIDZE M.
QUANTUM THERAPY IN PATIENTS WITH CYTOMEGALOVIRUS
J. Experimental and Clinical Medicine, 2007, 6, pp. 41-42.
Tbilisi State Medical University, Georgia
We have analyzed clinical-laboratory data from patients at risk of abortion caused by Cytomegalovirus in the I trimester of pregnancy. The patients were divided into two groups.
In group I (control), 15 possible abortion patients with cytomegalovirus infection were treated by traditional therapy. Group II (basic - 45 patients) was treated by traditional therapy plus therapy with photons (“Father” apparatus). Clinical trial was designed as a randomized distributed group (Method of Convert).
This new treatment-prevention method gives us the opportunity to increase treatment efficacy resulting in pregnancy prolongation and normalization of the immunological parameters.
8. GORDELADZE D., KANDASHVILI T., DVALI M., KHARDZIANI S., CHKADUA V., ROBITASHVILI G.
THE ROLE OF A THERAPY CLINIC IN A MULTIPLE-DISCIPLINE HOSPITAL
J. Experimental and Clinical Medicine, 2007, 6, pp. 42-46.
Tbilisi Clinical Hospital I (“Therapy clinic”), Georgia
A study of hospitalization cases and patients’ nozologic structure in the “Internal Medicine Clinic” of Tbilisi N1 Hospital in 2001-2005 demonstrated a tendency to increased hospitalizations. The majority of cases fell on pulmonary, cardiovascular and gastrointestinal pathologies. Most patients in the Internal medicine clinic (either who were directly admitted to the clinic and those who are directed there by other healthcare providers), along with the leading diseases, were treated for several other pathologies, mainly chronic heart ischemic disease, arterial hypertension, and diabetes mellitus.
9. KIRAKOSYAN S., ARUSTAMYAN K., AZARYAN H., AHARONYAN A., HARUTYUNYAN A., BALAYAN L.
CONDITION OF THYROID GLAND IN PATIENTS WITH BENIGN TUMORS OF UTERUS AND OVARIES
J. Experimental and Clinical Medicine, 2007, 6, pp. 46-49.
Research Center of Maternal and Child Health Protection, Yerevan, Republic of Armenia
The aim of the study is to assess the condition of the thyroid gland in patients with benign tumors of uterus and ovaries.
90 women aged 29-47 have been investigated, among them 60 with myoma and 30 with benign tumors of ovaries. The pathology of thyroid system have been registered in 72% of patients with myoma uteri. The pathology of thyroid system have been registered in 53,3% of patients with benign tumors of ovaries.
It is concluded that assessing the condition of the thyroid gland and its adequate therapy according to the results of thyroid system investigation for patients is important these.
10. RATIANI L., PETRIASHVILI E., QARCHAVA G., JARIASHVILI T., MUSELIANI T.,
CHACHUA M., NIKOLAISHVILI M.
INFLUENCE OF BLACK SEA POLLUTANTS ON POPULATION HEALTH
J. Experimental and Clinical Medicine, 2007, 6, pp. 49-51.
Scientific Center of Radiobiology and Radiation Ecology;
Sokhumi State University, Faculty of Exact and Natural Sciences, Tbilisi, Georgia
Studies of the influence of the Black Sea east coast pollutants on population health have shown that microbiological pollution in the studied zones might be characterized as epidemiologically non-hazardous or moderate. This might be explained by self-cleaning processes in the Black Sea, and might be considered safe for human health.
11. KOBERIDZE K., GAGUA R., TOPURIA I., KOUPRADZE V., CHIKVASHVILI A., KUCHAVA V., GUNDISHVILI J., GACHECHILADZE A., KORSANTIA B.
CLINICAL AND EXPERIMENTAL STUDY OF VULNERABILITY AND HEMATOPOETIC ACTIVITY OF PREPARATION “RUBIA”
J. Experimental and Clinical Medicine, 2007, 6, pp. 51-53.
Institute of Medical Biotechnology;
A.Gvamichava National Oncological Centre;
LTD “Rubia”, Tbilisi, Georgia
Investigation of the phytopreparation “Rubia” on the experimental animals revealed its harmlessness, absence of pyrogenic and locally irritating action (allergization), also the ability to improve some blood indices i.e. hematopoietic effect. Clinical analysis showed the expressed hematopoetic activity of the preparation and the improvement in some symptoms among the patients undergoing chemotherapy and radiation therapy.
12. PETRIASHVILI E., NIKOLAISHVILI M., CHACHUA M., MUSELIANI T., VASADZE L.
J. Experimental and Clinical Medicine, 2007, 6, pp. 53-55.
Sokhumi State University, Faculty of Exact and Natural Sciences, Tbilisi, Georgia
Exposure to cellular phone frequency electromagnetic fields provokes significant changes in the formation and storing of passive avoidance reaction in rats. Namely, in exposed rats the development of passive avoidance reaction is significantly decreased. The results obtained enable us to conclude that fragment 4-7 of ACTH transforms passive avoidance reaction.
13. KOUPRADZE S., MKERVALISHVILI J., DZOCENIDZE L., KORSANTIA B.
INFLUENCE OF PLAFERON ON REPRODUCTION OF ONCOVIRUSES
IN HEP-2 CELL CULTURES
J. Experimental and Clinical Medicine, 2007, 6, pp. 55-58.
Institute of Medical Biotechnology, Tbilisi, Georgia
There was studied the influence of placental interferon on the reproduction of onkoviruses of type D in the culture of the HEp-2 cells by calculating the number of different stages of virions to 10 outline- cells.
Interferon contributed to the sharp reduction in the intensity of onkoviruses multiplication. In comparison with the control, in limits of 50% was reduced the quantity of intra- and extracellular viruses, and the level of budding virions decreased almost by 75%.
14. MIMINOSHVILI M., SABAKHTARASHVILI M.
ULTRASTRUCTURAL PECULIARITIES OF UTERINE LEIOMYOMA
J. Experimental and Clinical Medicine, 2007, 6, pp. 58-61
Department of Fundamental Medicine of State Medical Academy, Tbilisi, Georgia
Background. Uterine leiomyoma is one of the most common solid tumors in adult women affecting at least 30% in their reproductive years. Despite its prevalence, little attention has been directed toward the causes and pathogenesis of this disorder. Uterine fibroids rank as the major reason for hysterectomy in the world, accounting for approximately one third of all hysterectomies.
Materials and Method. Human myometrial and leiomyoma tissue was obtained from 60 women after hysterectomy. Tissue samples were investigated by light and electron microscopy.
Results. Microscopically, the tumors were formed by interlacing bundles of spindle cells arranged in a whorl-like pattern. The vascular network is less abundant in the leiomyoma nodes. Endothelial cells show sings of minimal functional activity (luminal edge has no microvilli, vesicles are few and vacuole-like, basal lamina is degraded, nuclei are shrunken).
Conclusion. Endothelial dysfunction can play a pivot role in the pathogenesis of leiomyoma. Functional inactivity and insufficient transendothelial transport activity cause tissue ischemia which is a promoter of stromal cells proliferation.
15. VADACHKORIA D., GABUNIA L., BEZHITASHVILI N., SHANIDZE L., ASATIANI N.
ANALYSIS OF SPECIFICITIES OF WITHDRAWAL CONDITION DEVELOPED AS A RESULT OF COAXIL USAGE
J. Experimental and Clinical Medicine, 2007, 6, pp. 61-64.
Tbilisi State Medical University, Tbilisi, Georgia
In recent years, the information has been spread about coaxil dependency. coaxil usage for the purpose of achieving narcotic euphoria has sharply increased in Georgia .
24 patients with the diagnosis of coaxil dependence (withdrawal [abstinence] condition) were treated in the scientific-research institute of narcology.
There is no data about withdrawal condition of coaxil usage and its treatment methods in the modern medical scientific literature. 24 voluntary patients under clinical observation were opiate (Opium, Heroine, Subutex) users for the past 5 month.
Coaxil was administered in combination with some antihistaminic preparations (Pipolphen, Suprastin). The research was conducted using clinical–psychopathological and laboratory methods.
After comparing the withdrawal conditions of coaxil and opiate, it is clear that the condition caused by coaxil is characterized by high emotions and strong depression; during opiate withdrawal condition algetic and vegetodistonic symptoms predominated. Detoxication of the patients was conducted with algetic preparations, antidepressants, neuroleptics, clonidine, tranquilizers. Hemotransfusion and vitamin therapy were also provided to the patients.
Thus, the aim of our research was to provide the medical society and narcologists with the information about potential complications developed after coaxil administration with no medical purpose.
16. GOGNADZE T., MUKHADZE I., JINCHARADZE D.
FATHEROTHERAPY IN PATIENTS WITH GENITAL HERPES
J. Experimental and Clinical Medicine, 2007, 6, pp. 64-65.
Tbilisi State Medical University, Georgia
In this study we have analyzed the method of clinical-laboratory and physical study conducted on the patients at risk of herpes-caused abortion in the I trimester of pregnancy. 49 pregnant women were divided into two groups: group I (control) consisted of 11 possible abortion patients with herpes infection - they were treated by traditional therapy. 38 patients in the II basic group were treated by traditional therapy plus Fatherotherapy, clinical trial was designed as a randomized distributed group (Method of Convert).
Having analyzed this new method, we can claim that it is simple, available, easy to use, noninvasive, and allows increasing the treatment efficacy.
17. SHVELIDZE KH., BREGVADZE N., TABAGARI S.
LEUKOCYTE COUNT AND OTHER INFLAMMATORY MARKERS IN CORONARY HEART DISEASE AND UNSTABLE ANGINA PATIENTS WITH METABOLIC SYNDROME
J. Experimental and Clinical Medicine, 2007, 6, pp. 64-65.
“AIETI” High Medical School, Tbilisi, Georgia
Background. The leukocyte count has been shown to be an independent risk factor and prognostic indicator of future cardiovascular outcomes. In patients with a history of cardiovascular disease (CVD), it is an independent predictor of future events. Moreover, the leukocyte count is inexpensive, reliable, easy to interpret, and ordered routinely in inpatient and outpatient settings.
We wanted to compare leukocyte count and other inflammatory markers in Unstable Angina (UA) patients with and without Metabolic Syndrome (MS), the associations between leukocyte count and MS components.
Methods and Results. Patients with UA were divided into groups +MS and –MS (defined by Third Report of National Cholesterol Education Program Adult Treatment Panel). None of the selected patients was on therapy to lower lipids at the time of sampling. Total leukocyte count, differential leukocyte count, routine blood chemistry and lipids (TG, TC, HDL-C, LDL-C, VLDL-C, various atherogenic indexes) were analyzed. Levels of oxLDL were analyzed by enzyme immunoassay (ELISA), by MED.DIAS.R.L Kit for determining the amount of human autoantibodies to ox LDL directly in the serum. CRP measurement was performed, using CRP-Latex assay in a high sensitive application.
In the MS patient group, significantly higher were: Total leukocyte count (7,53±0,05 vs 6,73± 0,28); Neutrophile differential count (4,81± 0,22 vs 4,17± 0,22); TC; TG; LDL-C; AI(TC-HDL-C)/HDL-C (p<0,05); AIP- logTG/HDL-C (p<0,05); oxLDL/HDL-C (85,96± 4,46 vs 71,51± 4,65; p< 0,05). Patients’ division into quartiles on the basis of CRP level were shown, that it positively correlated with TC, TG; negatively – HDL-C.
When stratified patients with MS and without it into tertiles based on admission Leukocyte Count, a significant relationship only with waist circumference were found.
Conclusions. Leukocyte Count, hsCRP, oxLDL, AIP, oxLDL/HDL-C indexes are integral tests, which allow the possibility to consider atherosclerosis as inflammation. Measuring these tests has a prognostic significance in patients with MS. The index of Atherogenicity oxLDL/HDL-C has further improved the diagnostic accuracy of oxLDL. These tests will become the objects of therapeutic intervention and criteria for the evolution of medical treatment.
18. LUTIDZE R., TKESHELASHVILI B., BURKADZE G., SURGULADZE B.
EXPERIMENTAL/MORPHOLOGIC PECULIARITIES OF UTERINE TUBES VISUALIZATION DURING “UNIMAG” PREPARATION USAGE
J. Experimental and Clinical Medicine, 2007, 6, pp. 65-71.
Tbilisi State Medical University;
Tatishvili Medical Center;
Ltd N.Kipshidze University Central Department of Clinical Pathology;
Ltd “ATT” – Reserch Laboratory “Magnetic liquids in Medicine and Biology” Tbilisi Georgia
The goal of the research was the influence of the ecocontrast magnetic liquid “Unimag” on the mucous lining of the uterine horns and the study of its elimination.
Experimentally, female adult rabbits (27 in total) which had prevously undergone knotting of the uterine horn with the purpose of modeling tuboperitoneal infertility were injected via catheter with diluted and undiluted solutions (1:10, 1:20) of 5 mlg unimag. For the morphologic research material was taken on the 1 3 5 day after the preparation intake.
The results obtained:
1. Elimination of unimag particles is eccomplished via the mononuclear/phagocitic system, and the final organ of elimination is the liver.
2. The period of complete elimination of unimag covers 5 days.
Unimag (undiluted as well as diluted) practically does not damage the mucous lining of the uterine horn and is not subjected to cumulation in the organism.