EXPERIMENTAL & CLINICAL MEDICINE
2008, ¹ 2
1. PANTSULAIA T. CHOKHONELIDZE I.
INSULIN ADSORPTION TO PERITONEAL DIALYSIS BAGS
J. Experimental and Clinical Medicine, 2008, 2, pp. 9-11.
Al. Tzlukidze National Center of Urology
Department of Endocrinology, Tbilisi State Medical University, Georgia
Intraperitoneal insulin administration is very important for diabetic patients to control blood glucose level and to prevent development of peripheral hyperinsulinemia, which increases the risk of atherosclerosis. But when insulin is instilled into the peritoneal cavity along with the dialysis solution, switching from subcutaneous to intraperitoneal administration entails an increase of insulin requirement by approximately 30%.
Higher insulin requirements with intraperitoneal delivery, compared to subcutaneous administration, during CAPD is due to several factors, and one of them might be adsorption of insulin to the surface of fluid containers and connecting tubes.
According to our results, the amount of insulin adsorption is very low, and it could not play an important role in large insulin requirements in diabetic patients receiving intraperitoneal insulin.
2. KHETSURIANI R., SHUKAKIDZE A., ARABULI M., KHOMERIKI C., KIPIANI T.
AGE-RELATED CHANGES IN ERYTHROCYTE MEMBRANE PERMEABILITY AND THEIR INFLUENCE ON ERYTHROCYTE DIAMETER
J. Experimental and Clinical Medicine, 2008, 2, pp. 11-14.
Tbilisi State Medical University, Georgia
The research objective was to study age-related changes in erythrocyte membrane permeability and their influence on the erythrocyte diameter. According to age, membrane permeability changes, this influencing Na+ and K+ ions content in erythrocytes. On the other hand, this fact influences erythrocyte diameter changes.
Studies have been performed on the three age groups: newborns (umbilical cord blood), adults (22-30 years old) and the elderly (75-89 years old). Based on the results, we can assume that K+ content is less in the erythrocytes of newborns than in elderly people. That causes erythrocyte hydration, size and diameter increase in newborns. In the elderly, erythrocyte diameter remains almost unchanged, but K+ content in the erythrocytes of the elderly increases and signifies their high-resourcefulness. The content of Na+ ions remains unchanged in newborns, while it increases in the elderly in comparison with adults. This is caused by the inhibition of Na+ pump as a result of the accumulation of axes intercellular Ca2+ ions.
3. RaGimov V., Ismaylov I., Magerrambeyli I., Mamedov R., Mirzoeva V.
PECULIARITY OF PROVIDING ARTIFICIAL VENTILATION OF LUNGS IN DIFFERENT REGIMES TO RESUSCITATION PATIENTS WITH CRANIOCEREBRAL INJURY
J. Experimental and Clinical Medicine, 2008, 2, pp. 14-17.
Resuscitation and Intensive Therapy Department, Clinical Medical Centre,
Az.SMU, Baku, Azerbaijan
A target of the researches was studying of the condition of lung homodynamic and its changes in different regimes of artificial ventilation of the lungs. The researches have been conducted on 20 patients with intracranial hematoma and brain contusion, and have showed that artificial ventilation of the lungs in PCV regime lowers the pulmonary artery systolic pressure, common pulmonary resistance, and central venous pressure; assists to reduce the intracranial pressure and to increase the cerebral perfusion pressure. Therefore artificial ventilation of the lungs in PCV regime is pathogenetically well-grounded in patients with severe craniocerebral injury.
4. LOMSIANIDZE T., SAKANDELIDZE R.
Comparative analysis of physical development of newborns in Georgia
J. Experimental and Clinical Medicine, 2008, 2, pp. 17-20.
A. Tzereteli State University, Kutaisi, Georgia
The analysis of newborns’ physical development was carried out on the basis of observations performed in the maternity hospital “Beau Monde” Ltd. We considered records of one fetus deliveries (full term newborns of both sexes) over the period 2002-2006. The analysis was performed according to the following parameters: weight, height, head/chest circumference. The results were compared to the related data of the 70-ies and 80-ies of the last century. The positive dynamics was observed in relation to height, head and chest circumference; however, in relation to body weight it shows a tendency to decrease. Despite the last fact, according to the records, we can assume the existence of acceleration process. In order to get more precise results we continue an individual and detailed analysis of the data.
5. BUBASHVILI M., RIGVAVA S., GOGIASHVILI D., KARUKHNISHVILI M.,
VARDZELASHVILI N., NATIDZE M., LASAREISHVILI B., MAGLAKELIDZE T.,
GUBELADZE L., KAVTARADZE. L.
METHODICAL APPROACHES TO THE DEVELOPMENT OF COMBINED ANTI-ANTRHAX PREPARATION CREATING
Report 2. Creation of anti-anthrax toxin (AAT) hyperimmune serum and study of its
J. Experimental and Clinical Medicine, 2008, 2, pp. 20-23.
G. Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
Anthrax toxin (AT) plays a key role in Anthrax pathogenesis and in the formation of acquired immunity. Exotoxin is characterized by lethal, oedematic, protective and immunodepressive activities. B. anthracis exotoxin plays a significant role in the microbe virulence, disease pathogenesis, and causes potential infections induced by vaccinal strains.
The authors derived toxic culture filtrates (TCF) from the following vaccinal strains: STI-1; 55; 34 F2. After the verification of lethal and oedemic activities of the isolated TCF, they were used for hyperimmunization of rabbits. Serum activity was studied by precipitation test in agar jelly. The accumulation of specific antitoxic antibodies in blood was evaluated by the presence and level of precipitation expression lines. The study of the activity of the sera obtained on the 10th day after the final doses of the antigens demonstrated the following: in the sera to be studied, after 48 h, one clear precipitation line was revealed against TCF STI-1, and after 72 h - against TCF 55 and 34 F2.
The data obtained showed that hyperimmunization of the rabbits with vaccinal strains and their toxic culture filtrates was accompanied by the accumulation of the precipitating antibodies in the animal organism after the I hyperimmunization cycle.
6. KHIDIROV E.
hystochemical and immunohystochemical peculiarities of blood vessels of high cancer line mICE during benigN GROWTH and cancer of mammary glands
J. Experimental and Clinical Medicine, 2008, 2, pp. 24-28.
Department of Human Anatomy of AMU, Baku, Azerbaijan
Pathomorphological Laboratory of the A.Natishvili Institute of Morphology, Tbilisi, Georgia
In the mammary glands of mice aged 2.5-3 months, an investigation determined structures with proliferating epithelial cells. Nearby was observed a vascular net, braided with a net of broadened vessels, mainly capillaries. Also were observed perivascular oedema, vessel tortuosity, hyalinosis of vessel walls, mainly of arterial-type vessels.
Angiogenesis occurred near the proliferating glandular structures, confirmed by labeling of endotheliocytes with the markers of proliferation Ki67 and angiogenesis CD31.
The endothelial cells of precancerous growth in the mammary glands of these mice include a small amount of NAP as drops of rosy color. In the middle and external coats, there are found lots of PAS–positive not amylase-repressed, but acetylation-repressed materials. Staining of NAP is homogenous, very intensive, especially in the walls of arteriols, PAS-positive stain intensifies in the vessels with thickened walls.
The connective tissue, comparatively loose fibers located around the vessels are weakly stained, repressed by acetylation.
So, in the experimental material exposed, the increasing age of the high cancer line mice and developed cancer of the mammary glands strengthen angiogenesis and blood supply to pretumorous growth and cancer tissue.
7. CHIKVILADZE D., METREVELI D., GACHECHILADZE KH., MIKELADZE M.
MICROBIOLOGICAL ASPECTS OF CHRONIC PROSTATITIS DIAGNOSIS
J. Experimental and Clinical Medicine, 2008, 2, pp. 28-31.
Department of Microbiology and Immunology, Tbilisi State Medical University, Georgia
Chronic prostatitis/chronic pelvic pain syndrome is one of the most common diseases in urology which is difficult to diagnose and treat. According to current international guidelines, a choice of the approach to the management of patients with chronic prostatitis depends on localization of pathological process in the urinary tract, clinical type of the disease, and etiology. As a result of microbiologic investigation of 128 patients with diagnosis of chronic prostatitis, positive results were observed in 95 men (74.2%). Among isolated 103 microbial strains, 37 (35.9%) belong to gram-positive microbial flora and 66 strains (64.1%) - to gram-negative. Isolated gram-negative microflora has twice as much prevalence over gram-positive. Investigation that has been carried out confirms the necessity of further microbiological investigations for detection of a correct etiology of the disease, that in its turn minimizes the risk of irrational antibiotic therapy.
8. JAFAROVA U.
macro-microscopic characteristics and quantitative indexes of HUMAN esophagEAL glands proper in different age groups
J. Experimental and Clinical Medicine, 2008, 2, pp. 31-36.
Department of Human Anatomy, Azerbaijan Medical University, Baku
An investigation has been carried out on 154 corpses (both male and female sexes, from newborns to the elderly) to determine localization and character of the esophageal glands. After releasing the esophagus from the adventitia and muscular layers, it was stained with 0.05 % methylene blue: this being performed for 24-36 hours at room temperature. After staining, the preparation was immersed into the ammonia solution.
The macro-microscopic investigations showed that characteristics of the esophageal glands largly depend on their form and size. They have initial portions and excretory ducts. The initial portions of the oesophageal glands are enlarged in newborns. The parameters of the organ change and the resistance of its wall increases toward the first third of the lower area. This can be an important point for the esophageal glands.
9. KEZELI T., GONGADZE N., DOLIDZE N., ABULASHVILI D.
The action of zafirlucast on THE vascular relaxing system and erythrocite osmotic resistance in epinephrine induced heart injury
J. Experimental and Clinical Medicine, 2008, 2, pp. 36-43.
Department of Pharmacology, Tbilisi State Medical University, Georgia
The effect of the cysteinal-leurotriene antagonist - zafirlucast - on the hemodynamic changes, baroreflex symphatetic cardiochronotropic component sensitivity (BRSCCS), vascular relaxing system and erythrocyte osmotic resistance (EOR) was observed in 48 rabbits after 24 hours, within 5 and 10 days of epinephrine induced heart injury. It was shown that cardiac damage was accompanied with a decrease of BRSCCS, EOR and vascular endothelial dysfunction. Pretreatment with zafirlucast increased BRSCCS, EOR, and potentiated the effect of nitric oxide endogenous donor - L-arginine. The modulatory effect of zafirlucast more markedly was revealed after a 5-0 day period of heart injury. Guanylatcyclase inhibitor - methylen blue and LNG-nitroarginine prevented the modulatory action of zafirlucast on the above - mentioned parameters. It is suggested that leukotrienes may play a significant role in stress-induced heart damage, and zafirlucast can produce a beneficial effect in such conditions.
10. NIKOLAISHVILI M., VASADZE L., SOLOSHVILI T., MUSELIANI T., PETRIASHVILI E., JARIASHVILI T.
THE EFFECT OF COMMUNICATION FREQUENCY ELECTROMAGNETIC FIELDS ON ANIMAL BEHAVIOR AND DISTRIBUTION OF AMINO-ACIDS IN THE CORTEX OF HEMISPHERES
J. Experimental and Clinical Medicine, 2008, 2, pp. 43-47.
Center of Radiation Biology and Ecological Radiology, Tbilisi, Georgia
The exposure to communication frequency EMF causes significant changes in concentrations and distribution of amino-acids in the cortex of hemispheres. The rats exposed to communication frequency EMFs are characterized by low emotionality, which is manifested in the reduced number of boluses and urination, and reduced translocation. These changes are accordingly reflected on their behavior in “Open Field”.
11. CHOMAKHASHVILI Z., PHUTKARADZE M., MIKELADZE A., KHAZHALIA T.,
SURGICAL TACTICS IN TRAUMATIC SPLENIC INJURIES
J. Experimental and Clinical Medicine, 2008, 2, pp. 47-50.
Batumi Central Clinical Hospital, Georgia
After splenectomy, the physician is faced with the necessity of adequate correction of the complications occurred.
At the same time, it is necessary to take into consideration that spleen-sparing operations are limited because of the hazard of inadequate hemostasis development. Several examples are known when in spite of all the possible attempts there is no way to preserve even part of the spleen, and splenectomy becomes unavoidable. In such conditions, only functional maintenance of the organ is possible. In view of the aforesaid, correct surgery tactics should be chosen by the surgeon for traumatic splenic damage, so far as this is the most important task having the earlier as well as the long-term influence on the surgical treatment results.
The above-mentioned investigation is based on the results of 97 patients’ treatment, and the authors conclude that splenectomy, which is accompanied by rather serious surgery aftereffects can not be considered the unalternative treatment method for traumatically damaged, but otherwise healthy spleen at the present stage of scientific and technical development of surgery. Priority should be given to the sparing operations or to the spleen functional preservation by spleen tissue autotransplantation in “desperate” situations.