J. Experimental and Clinical Medicine, 2009, 10

Abstracts

1. Petriashvili Sh., Gabunia L., Kuridze N., Saralidze N., Gelashvili N.

Heart Insufficiency and recent approaches TO ITS treatment

J. Experimental and Clinical Medicine, 2009, 10, pp.12-19

 Tbilisi State Medical University; I. Javakhishvili Tbilisi State University, Georgia

Heart insufficiency is a clinical syndrome with the background of pericardial, myocardial, endocardial or great vessels disorders, but this condition is generally related to the left ventricle dysfunction.

Heart insufficiency pathogenesis must be taken into consideration during treatment of the disease. Effective treatment includes two directions: symptomatic and pathogenetic. Hospital treatment of the disease is accomplished via intravenous administration of several combined preparations: active diuretics, β-adrenomimetics, inhibitors of phosphodiesterase, as well as vasodilators (Sodium Nitropruside or Nitroglycerin). In outpatients (no need of urgent medical treatment) therapy is carried out with diuretics, β-adrenoblockers, vasodilators (inhibitors of the renin-angiotensin-aldosteron system). So far ACE-inhibitors (inhibitors of the angiotensin-converting factor) as nonselective vasodilators are the gold standard of heart insufficiency therapy (Captopril, Enalapril, Lizinopril, Fozinopril, etc). 

β-adrenoblockers were counterindicated for the treatment of heart failure because of their inotropic effect, but the recent multicentral researches have estimated that systematic usage of β-adrenoblockers (Metoprolol, Bisoprolol, Carvedilol) improves patients’ health condition and reduces the risk of mortality. On the bases of a huge number of researches it has also been established that cardiac glycosides increase the mortality indicators, but decrease the symptoms of disease as well as the number of cases for hospitalization.

Heart failure therapy must be prolonged; improved health condition of a patient is not a sufficient reason for discontinuing medication therapy. Such therapy must be conducted along with supporting treatment, and each patient should be under dispensary supervision.

2. Gongadze M., Chkhikvishvili I., Gogia N., Esaiashvili M., Aslamazishvili T.,

Meladze T., Mesropyan I.

Red wine extract as teratoGENIC protector

J. Experimental and Clinical Medicine, 2009, 10, pp.19-22

Institute of Medical Biotechnology, E. Andronikashvili Institute of Physics, Tbilisi, Georgia

The generation of reactive oxygen species (ROS) is presently seen as one of the key events which can lead to the formation of inborn structural anomalies in embryos exposed to detrimental maternal stimuli or environmental teratogens. Therefore, antioxidants might increase embryonic resistance to ROS-generating teratogens. Beside, to antioxidant/antiradical activity, red wine polyphenols (RWPs) were shown to possess many biological properties. We performed a teratological study in mice exposed to a ROS-generating teratogen, cyclophosphamide and an antioxidant, red wine extract. Summarizing of the experimental results obtained in mice treated with CP, we can conclude that an antioxidant, red wine extract, acts as a protector against CP-induced teratogenic stimuli. 

3. Askerova D., Huseynova S., OruJova P.

Modern aspects of studies of cns hypoxic-isChemic injury in newborns

J. Experimental and Clinical Medicine, 2009, 10, pp.22-28

Department of Neonatology of Azerbaijan State Medical University, Baku

High percent of scientific studies is performed every year, which confirm the actuality of brain damages in modern medicine. This article is based on the results of different researches on central nervous system hypoxic-ischemic injury in the perinatal period.

We have glanced at the role of biomarkers of intracellular adhesion molecules, vascular-endothelial factors and other specific proteins taking part in neuronal tissue inflammation. There factors are very important in the diagnosis and prognosis of perinatal and neonatal brain damages.

4. Yener О., Aliyeva E., Abbasova F., Bije M., AHundova T.

PECULIARITIES of UTERINE ACTIVITY IN spontaneous DELIVERY and during preparation of cervix with vaginally administered misoprostol

J. Experimental and Clinical Medicine, 2009, 10, pp.28-31

III Department of Obstetrics & Gynecology, Azerbaijan State Medical University, Baku;

Clinic of Delivery and Gynecology Diseases, Kahraman Marash, Turkey

The purpose of the given work is to investigate uterine activity in spontaneous delivery uncomplicated with the anomalies of labor and in the process of cervix preparation with vaginally administered misoprostol. The method of external cardiotocography was used for the investigation. The investigation results showed that the use of vaginal misoprostol (50 mg) is very effective for cervix ripening and for labor induction. At the same time it may be a reason for acute hypoxia of the fetus.

5. AGAEV I., SHUKHUROVA E.

REALIZATION OF THE PRECAUTIONARY PRINCIPLE IN THE IMPLEMENTATION OF THE STATE SANITARY-EPIDEMIOLOGIC SURVEILLANCE OVER FOOD-BORNE TOXICOINFECTIONS (ACCORDING TO THE BAKU MATERIALS)

J. Experimental and Clinical Medicine, 2009, 10, pp. 31-36

Department of Epidemiology, AMU, Baku

Morbidity rate due to food-borne toxicoinfections is attributed to the reservoirs for infection which might be men, animals and environment. This rule forms the basis for the organization of precautionary sanitary surveillance.

6. Mikailova S., Faradzhov A., Safarov M.

GABA TURNOVER IN MITOCHONDRIAL FRACTIONS OF CNS STRUCTURES IN POSTNATAL ONTOGENESIS UNDER THE IMPACT OF ETHANOL

J. Experimental and Clinical Medicine, 2009, 10, pp. 36-42

Department of Human and Animal Physiology, Azerbaijan State Pedagogical University, Baku

The effect of GABA turnover has been studied in the mitochondrial fractions of brain structures under acute poisoning with ethanol in 6 periods of postnatal ontogenesis. It was revealed that a considerable increase in GABA level took place in the mitochondria of the cerebral cortex, cerebellum, brain stem and hypothalamus after the impact of a high concentration of alcohol. In this case, GDC (glutamate decarboxylase) activity increases, while the activity of GABA aminotransferase (GABA-T) gets suppressed in 10- and 21-day-old animals; in other periods of postnatal development it increases considerably. The maturity of CNS structures has a special importance for the manifestation of changes in the activity of GDC, and especially GABA-T, under the impact of highly concentrated alcohol. The absence of parallelism between the changes in GABA, free Glu, Asp levels, the activities of GDC and GABA-T enzymes indicate that the ratio of substrate-enzyme in the GABA system of the studied brain structures mitochondrial fractions changes under the impact of a high ethanol concentration. However, they change differently in different periods of postnatal ontogenesis. The increase in GABA level and in the activity of GDC and GABA-T enzymes in response to acute intoxication with the high ethanol concentration (3.3 g/kg of 25% ethanol solution, intraperitoneally) is considered as protective-adaptive and compensatory reactions of the mitochondrial fractions of the studied brain structures and of the entire body.

7. Kldiashvili I., Moseshvili T.

Local anti-INFLAMMATOry treatment for periodontitis with

the AID of new structure DENTAL FLOSSES

J. Experimental and Clinical Medicine, 2009, 10, pp. 42-45

Kutaisi Dental Polyclinic №1, Department of Textile Technology and Design, Kutaisi A.Tsereteli State University, Georgia

Pathological microflora exerts an effect on the development of the inflammatory process in the parodentium tissue. The antibiotics clindamycin and metronidazole have been applied in local treatment for periodontitis. Two groups of patients with the generalized form of perodontitis have been investigated. Frequent growth of pathological microbes was determined through microbiological investigations. The patients from the first group underwent treatment with the above-mentioned medicines along with sodden turunda, but another group of patients was provided with the same medicines via two flosses of different thickness – cotton untwisted yarn. The thread impregnated with metronidazole was placed into the bottom of a periodontal pocket, and another thread impregnated with clindamycin was placed above the first one. They were applied for 20 minutes; 10 sessions were conducted.

The microbiological investigations carried out after the treatment have shown that as a result of treatment by the method of two flosses (untwisted cotton yarn) pathological microflora was essentially slackened or absolutely disappeared; after treatment with sodden turunda, the growth of pathological microbes remained at a medium level.

8. Ahmedov F.

SOCIAL/ECONOMICAL FUNDAMENTALS OF PREVENTIVE MAINTENANCE OF INFLAMMATORY DISEASES OF THE MIDDLE EAR

J. Experimental and Clinical Medicine, 2009, 10, pp. 45-49

Mir-Kasimov Republican Clinical Hospital, Baku, Azerbaijan

Till now, preventive maintenance of inflammatory diseases of the middle ear has been basically reduced due to the timely admission of the adult population for specialized medical aid in cases of the disease, and its maximum treatment. The results of the performed work show that due to the implementation of scientifically-proven preventive measures an effective preventive maintenance of inflammatory diseases of the middle ear has been attained. In particular, the risk of the disease decreases 4.18 times. At the same time, the organization and conduction of the developed complex of preventive measures is accessible, does not demand additional personnel and financial-technical resources, and is attained by sanitary propaganda among the population. Purposeful and constant explanatory work enhances the population’s responsibility for health, makes it active in implementing of the preventive measures, which finally creates real preconditions not only for stabilization of the morbidity level of the middle ear inflammatory diseases, but also for its consecutive decrease.

9. Shengelia M., Gogebashvili N., Mchedlishvili T., Intskirveli N., Sanikidze T.

Alteration of THE activity OF blood antioxidant enzymes during CHOLELITHIASIS in postmenopausal women

J. Experimental and Clinical Medicine, 2009, 10, pp.49-52

P.Shotadze Medical Academy, Tbilisi State Medical University, Georgia

Cholelithiasis belongs to the most widespread diseases of the gastroenteral tract. Taking into consideration the fact that cholelith production is often associated with the inflammatory processes connected with oxidative stress, we proposed that the alterations of oxidative metabolism constitute a very important pathogenetic link in the pathogenesis of cholelithiasis. The aim of our study was to establish alterations in the activity of blood antioxidant enzymes during cholelithiasis in postmenopausal women. The study result show that in postmenopausal women with olelithiasis the activity of antioxidant enzymes – SOD and GR decreases; at the same time a compensatory increase was revealed in the catalasa activity. The study results indicate the intensification of oxidative processes and disorders of the redox-balance in the patients’ organisms.

10. Agaeva N.

MICROBIOLOGICAL AND IMMUNOLOGICAL CHARACTERISTIC OF PARODONTITIS AND GINGIVITIS OF ACTINOMYCOTIC ETIOLOGY

J. Experimental and Clinical Medicine, 2009, 10, pp.52-56

Azerbaijan State Medical University, Baku

The aim of the research was to reveal the associations of actinomyces in the pathologic material from patients with the diagnoses of paradontitis, gingivitis, and other microorganisms.

The subpopulations of T- and B-lymphocytes have been researched in the blood to determine some immunoglobulins of cytokines.

The results of the investigation have shown that all indices in the immune system of the patients with actinomyces diseases were lower than normal.

11. Agamirova A.,  Mamedov R.,  Gasimov E.,  Safarova S., 

Mamedov A., Rustamov V.

Laser therapy in complex treatment of Helicobacter Pylori infection IN patients with bleeding duodenal ulcer

J. Experimental and Clinical Medicine, 2009, 10, pp.56-59

Azerbaijan Medical University, Baku

Today, the question remains still unresolved - which is the most effective scheme of eradication therapy (EТ) with minimum side effects.

The goal of the research was to estimate the efficiency of the application of low intensity laser irradiation in complex treatment for bleeding duodenal ulcer and its value in eradication therapy. The authors have come to the conclusion that EТ, including the complex of 3 triple therapy (Pariet, Amoxicillin, Claritromicin) and low intensity laser irradiation, is distinguished by its high (91.3 %) efficiency with minimum side effects and twofold reduction of a dose of the applied drugs.

12. Musaev E., Kerimova G., Mekhtieva G., Shirinova S.

MICROBIOLOGICAL STATUS OF THE ORAL CAVITY OF MENOPAUSAL WOMEN WITH INFLAMMATORY DISEASES OF PARODONTIUM

J. Experimental and Clinical Medicine, 2009, 10, pp.59-62

Azerbaijan Medical University, Baku

There was investigated the microbiological status of the oral cavity of 178 women in various stages of menopause and with parodontium diseases: among the women with gingivitis and parodontitis there was noted a high occurrence of pathogenic microbes (р<0.05) as compared with the control group made up of young and healthy women,

Complex treatment for the inflammatory diseases of the parodontium (IDP) covering replaceable hormonal therapy promotes IDP remission much better than treatment by standard methods.

13. SHAKARASHVILI M., JORBENADZE T., LOMAIA T.

HELICOBACTER PYLORI

J. Experimental and Clinical Medicine, 2009, 10, pp.62-68

I. Javakhishvili Tbilisi State University, “Interpharm+” University, Georgia

By many experimental and clinical screenings Helicobacter Pylori is proved to cause pathology in GIT. HP is considered to be one of the main factors in the development of chronic gastritis, gastroduodenitis, ulcus gastritis and ulcus duodeni, gastric adenocancer.

Many authors pay attention to this unique factor of the agent. More than 40 pathological genes are gathered in one segment of the chromosome - the phenomenon named “pathological island”. But permanent recombination between different cultures of HP leads to the genome instability. According to some authors, a variety of clinical infections are affected by the instability and changeability of HP. Perhaps it helps microbes to “escape” the immune system of a patient, and therefore, the immunological answer to HP is insufficient. Besides, a changeable microbial genome helps to resist antibiotics.

At present, HP infection is considered to be the main cause of chronic gastritis, gastric cancer, stomach ulcer and stomach cancer.

14. GULIYEV CH., MAHAMMADOV V.

СORRECTION OF CHANGES IN THE LPO-AOP SYSTEM OF CHILDREN WITH HIRSCHPRUNG’S DISEASE

J. Experimental and Clinical Medicine, 2009, 10, pp.68-72

Department of Pediatric Surgery, Azerbaijan State Medical University, Baku

The indications of lipid peroxidation (LPO) and antioxidant protection (AOP) have been studied in 30 patients with Hirschprung’s disease. The patients were divided into 2 groups: group 1 included 12 patients traditionally treated before the operation; group 2 included 18 patients who underwent correction with the help of a new, pathogenically grounded method, along with the preoperational preparation.

In all patients who had admitted to the clinic, there was observed an increase of LPO products contrary to the weakening of the AOP indicators activity. 

In the period of preoperational preparation, prophylactic antioxidant therapy resulted in the decrease of lipoperoxidation products and in the increase of the AOP indicators activity in comparison with the group 1.

15. Koupradze S., Mkervalishvili J., Dzotsenidze L., KAJAYA L.

THE Influence of Plaferon LB on THE ApoptoTiC Activity and Morphology of Transformed Human Cells

J. Experimental and Clinical Medicine, 2009, 10, pp.72-74

Institute of Medical Biotechnology, Tbilisi, Georgia

Plaferon exerts an expressed anti-proliferative action on tumour cells. Under the influence of the preparation, the quantity of СаОv cells in 24 hours of cultivation has decreased by 35%, and in 48 hours - by 56% in comparison with the same control cells. The mechanism of similar influence is connected with restoration of the apoptotic ability of transformed cells and with the decrease in the speed of their mitosis.

The anti-proliferative activity of Plaferon was not observed in connection with nontransformed cells, which gives us the right to recommend the inclusion of this preparation in the treatment for various diseases of tumorous genesis.

16. Kochakidze N., Mdivani N., Khetsuriani R.,  Shukakidze A., Kipiani T.

SOME ASPECTS OF REHABILITATION OF PATIENTS WITH ABERRANT GENOME

J. Experimental and Clinical Medicine, 2009, 10, pp.74-77

Department of Human Anatomy, Tbilisi State Medical University, Georgia

Rehabilitative influences of adaptive physical exercises are based on the interrelations of the impellent and vegetative functions. In particular, after correctly chosen physical exercises, according to the functionality of an organism, muscular activity causes positive changes in the functioning of internal organs. According to the comparative data from the research and control groups, we can conclude that the complex of adaptive exercises and correctly chosen physical load (taking into account duration of the impact) cause a comparative rehabilitation of the cardio-vascular system in people with congenital aberrant genes, which is very important for their vital activity.

17. Khvitia N., Davarashvili Kh., Zhvitiashvili T., Danelia G.

SHVANN CELLS IN THE NORM AND IN ACUTE PULPITIS

J. Experimental and Clinical Medicine, 2009, 10, pp.77-80

Tbilisi State Medical University, Department of Biology, Georgia

Shvann cells have been investigated in the norm and also during acute pulpitis with the help of light and electronic microscope. There were investigated 10 cases of the tooth pulp in acute pulpitis, and 5 cases of normal pulp. It has been established that miellin fibres are destroyed during acute pulpitis. This factor proves that electric impulses are directed in the wrong way – that’s felt as pain clinically.

18. Esaiashvili M, Chkhikvishvili I, Akhvlediani M, Sharashenidze G.

Effect of  chlorogenic acid on experimental model of diabetes mellitus

J. Experimental and Clinical Medicine, 2009, 10, pp.80-83

Institute of Medical Biotechnology, Tbilisi, Georgia

Progressing the diabetes mellitus maintains the further destruction of β-cells that is partly caused by the high level of glucose in blood and oxidative stress. The aim of the study was the investigation of chlorogenic acid action mechanisms in experimental (alloxan) diabetes mellitus. The experiments have been carried out on white rats. To induce the insulin diabetes, the animals were injected by alloxan.

Based on the experimental results it may be concluded, that chlorogenic acid decreases intensity of oxidative stress, normalises antioxidant enzyme (SOD) activity, glucose level in blood. The experiment verified corrective effect of chlorogenic acid on the parameters of lipid metabolism - blood cholesterol and LDL levels.