J.EXPERIMENTAL & CLINICAL MEDICINE
2011. ¹ 2
1. Gongadze M., Sepashvili A., Gogia N., Atamashvili Ts., Mesropyan I., Iobadze M.
IL-10 in pathogeneses of diabetes-induced pregnancy loss
J. Experimental and Clinical Medicine, 2011, 2, pp.8-12
TSMU Institute of Medical Biotechnology;
Tbilisi State Medical University;
E. Andronikashvili Institute of Physics, Tbilisi, Georgia
Spontaneous abortions and inborn structural anomalies are the main complications of diabetic pregnancy. We made a research to reveal the role of IL-10 in streptozotocin-induced diabetic outcome of pregnancy in IL-10-/- and IL-10+/+ mice. According to our results, we can conclude that IL-10 plays a major role in diabetic pregnancy outcome. A knock-out dose of Interleukin-10 makes pregnant mice much more sensitive to sterptozotocin-induced diabetes. We found out that they have a reduced pregnancy rate. IL-10 production decreases during diabetes and, therefore, the expression of TNFa increases in the uterine mucosal cells, leading to the increase of NO production. Accordingly, the processes of excessive apoptosis and reduced proliferation cause uterus weight loss. Consequently, the uterus is not ready for successful implantation; there also occur deregulation and dysfunction of important molecules (such as LIF and MMP-9) responsible for uterine sensitivity acquirement and implantation.
2. Dolidze I.
Sanology – A Fundamental teaching oN health care MANAGEMENT
J. Experimental and Clinical Medicine, 2011, 2, pp.12-17
Department of Medical Physics, Tbilisi State Medical University, Georgia
There are two strategies in the modern public health system: treatment of diseases and health protection in a healthy organism. The government considers the last one of a less priority because nowadays it’s more common to eradicate diseases than to preserve health. This situation has influenced doctors’ training in medical centres because Sanology is rejected in the educational system. Actually, pathogenic and sanologic reactions are both involved in a diseased organism i.e. disorganization and restoration reactions equally occur in the organism. With all ensuing consequences, psycho-physical condition and social standing of a healthy human must become the main object of medical researches; doctors should start keeping the “Health history”, establish the defensive-compensative mechanism of a healthy body in order to preserve health and recover disturbed homeostasis. According to this position, Sanology is a fundamental teaching on health management, which should be based on theoretical-practical achievements in pathology, rehabilitation, and general valeology.
3. Hayrapetyan A.
Àesthetic åffectiveness of rhinoplasty and fat grafting combination IN the particular facial zones
J. Experimental and Clinical Medicine, 2011, 2, pp.17-25
Medical Centre “Erebouni”, Yerevan, Armenia
In the particular facial zones, the combination of rhinoplasty and fat grafting is an effective procedure to achieve maximum aesthetic improvement of facial appearance. Autologous fat is compatible filler; anatomic and histological factors should be taken into account during its injection into the facial regions.
4. Joglidze N., Machavariani M., Enukidze M., Sanikidze T.
A STUDY of THE compound GR-805 AND ITS cytoprotective activity
J. Experimental and Clinical Medicine, 2011, 2, pp.25-28
Clinical trials have revealed the toxic activity of lead in the living organism. The aim of our study was to investigate the influence of the synthetic compound GR-805 on the Jurkat cell culture. The results show that the preparation is characterised by a cytoprotective activity in the Jurkat cells incubated under the conditions of low and moderate oxidative stress.
5. KANAYAN R., KANAYAN V.
EARLY RESULTS OF ENDOSCOPIC PAPILLOSPHINCTEROTOMY
J. Experimental and Clinical Medicine, 2011, 2, pp.28-33
Medical Centre «Erebouni», Yerevan, Armenia
The early results of 758 endoscopic papillosphincterotomies (performed in the period of 1987-2010) were analyzed retrospectively in the Erebouni Medical Centre. The disease was linked with the bile ducts in 67 (8,9%) patients, and 20 (29,9%) out of them had no clinical symptoms. It the early period, 43 (64,2%) patients were discovered, when in a later period - only 24 (35,8%). The above-mentioned can be explained by the active observation of the patients along with the application of all the complexes of instrumental methods of study in the early post-papillosphincterotomy period with the aim to reveal deflections which could serve as a cause of relapse of the disease and deterioration of life quality in the late period. A timely detection and liquidation of deflections led to the improvement of the remote results of endoscopic papillotomy.
6. Kanayan R., Kanayan V.
BLEEDING AFTER ENDOSCOPIC PAPILLOSPHINCTEROTOMY: A COMPARATIVE ANALYSIS, PROPHYLAXIS, TREATMENT
J. Experimental and Clinical Medicine, 2011, 2, pp.33-37
Medical Centres “Erebouni” and “Nairi”, Yerevan, Armenia
We have studied 2136 sphincterotomies and patients with post-sphincterotomy bleeding. In comparison with the initial stage (1987-1997), the percentage of bleeding cases decreased from 2.4% to 1.4% (1998-2010). The positive dynamics ensues from changes in the tactics of endoscopic interventions, including: well grounded indications to each manipulation; significant modification of the technique for a concrete procedure; scrupulous estimation of the initial condition of a patient before endoscopy.
7. Tchiokadze Sh., Galdava G.
Infectious inflammatory pathologies of the Urogenital System and seminal quality
J. Experimental and Clinical Medicine, 2011, 2, pp.37-40
Tbilisi “AIETI” Medical School; “Health House” Ltd.; Research Institute of Skin and Sexually Transmitted Diseases; I.Javakhishvili Tbilisi State University, Medical Department, Georgia
5 normospermic men with idiopathic infertility were revealed out of 85 patients fully examined for infertility. The rest of 80 patients had urogenital diseases of different etiology with chronic infectious inflammatory pathologies and pathospermia.
The present study has shown that infectious inflammatory processes as well as resultant complications in urogenital organs impair the ejaculate quality in regard to fertility. In particular, the ejaculate from all the patients diagnosed had a markedly decreased number of spermatozoa. Changes in the sperm volumes were seen in 32 (40%) of men with different inflammations of the genitalia. pH was altered in 62 (77%) cases, with a decreased pH noted in 39 (62%), while increased pH was in 11 (37.1%) patients.
It has been established that infectious inflammatory processes seriously affect the concentration of actively motile, especially of fertility –potent (forward progressive) sperm. The number of sperm leukocytes drastically rises resulting in leukocytospermia which has been documented in 29 (36.25%) cases. The study has revealed significant disturbances of spermatokinesis, increase in teratogenic shapes of spermatozoa, and changes in the biochemical indices of the ejaculate.
8. Hayrapetyan A.
COMPLICATIONS AFTER THE USE OF «MARLEX» POLYPROPYLENE MESH
FOR SECONDARY RHINOPLASTY
J. Experimental and Clinical Medicine, 2011, 2, pp.40-45
Maxillofacial Department, “Erebouni” Medical Centre, Yerevan, Armenia
Every surgical operation has a tendency to complications, and only the surgeon who does not operate faces no complications. The purpose of this study was to document a rare case of unfavourable result after the application of the polypropylene mesh “Marlex” to a secondary rhinoplasty patient. We present the surgical stages of a tertiary rhinoplasty with the excision of the inflamed polypropylene mesh and with the reconstruction of disturbed nasal anatomy, using ear cartilaginous grafts and bony grafts from the lower turbinate.
9. Kanayan P., Kanayan V.
PANCREATITIS AFTER ERCP: A COMPARATIVE ANALYSIS, PROPHYLAXIS
J. Experimental and Clinical Medicine, 2011, 2, pp.45-51
Medical Centres “Erebouni”, “Nairi”, Yerevan, Armenia
A total of 2136 consecutive ERCP procedures performed in 80 patients were analysed for the occurrence of pancreatitis. In comparison with the initial stage (1987-1997), the percentage of pancreatitis cases decreased from 42 (5.0%) to 38 (2.9%) (1998-2010). the positive dynamics consists of changes in the tactics of endoscopic interventions, including: well grounded indications to each manipulation; significant modifications of the technique for a concrete procedure; scrupulous estimation of the initial condition of a patient before endoscopy.
10. Kanayan R., Kanayan V.
A COMPARATIVE ANALYSIS OF COMPLICATIONS AFTER ERCPES
J. Experimental and Clinical Medicine, 2011, 2, pp.51-56
Medical Centre “Erebouni”, Yerevan, Armenia
The authors have carried out a comparative analysis of complications after ERCP/ES in the initial stage (1987-1997) and in the subsequent period (1998-2010). 182 patients with complications were investigated. The quantity of manipulations made up 2136. In comparison with the initial stage, the percentage of complications decreased from 11.2% up to 6.7 %. The positive dynamics consists of changes in the tactics of endoscopic interventions, including: well grounded indications to each manipulation; significant modification of the technique for a concrete procedure; scrupulous estimation of the initial condition of a patient before endoscopy.
11. Stepanyan S., Apoyan V., Drampyan A., Pogosyan S., Mesropyan R.
Laparoscopic resolution of early postoperative adhesive small bowel obstruction
J. Experimental and Clinical Medicine, 2011, 2, pp.56-61
Chair of Surgery ¹2, Yerevan State Medical University;
Surgery Clinic, Republican Medical Centre “ARMENIA”;
Republican Centre of Perinatology, Obstetrics and Gynecology, Armenia, Yerevan
The results of treatment of 14 patients (5 men and 9 women) with early postoperative adhesive small bowel obstruction are presented in the article. The average period from the previous operation till the intervention for bowel obstruction consisted of 12.1±7.8 /2-30/ days; the duration of intestinal obstruction history was 28.4±14.8 /8-76/ hours. Laparoscopic procedures were performed using laparoscopic set of “Karl Storz” company (Germany); the tools of “Karl Storz”, “Ethicon” /USA/, “Krilo” (Russia, Voronezh) were also applied. The first troacar was imposed only by the open method in the area most distant from the surgical wound. Obturation was detected in 8, strangulation - in 6 cases. In 12 patients obstruction was solved by the laparoscopic method, in two cases conversion was performed. In order to prevent adhesions of the abdomen, antiadhesive barriers Adept (4% Icodextrin solution) and Seprafilm (sodium hyaluronate + carboxymethylcellulose) were applied. There was no mortality. No recurrence of intestinal obstruction was noted during the period of observation.
Laparoscopic adhesiolysis provides minimal trauma and rapid postoperative recovery, which is especially important in the early postoperative period.
12. Verulashvili I., Samushia O., NogHaideli N.
Peculiarities and TREATMENT of cognitive impairments AFTER CEREBRAL STROKE
J. Experimental and Clinical Medicine, 2011, 2, pp.61-64
Department of Neurology, DKC Clinic of TSMU University, Tbilisi, Georgia
Methods: To evaluate a 3-year incidence of poststroke cognitive impairments (PCI) and the influence of prestroke cognitive decline, the authors evaluated prestroke cognitive functions in 173 consecutive stroke patients (³40 years old), using the Informant Questionnaire on Cognitive Decline in the Elderly (IQCODE). For six months and then annually after the stroke, dementia was reassessed. The diagnosis of dementia was based on the International Classification of Diseases, 10th revision criteria in survivors who visited the neurologist, or on the IQCODE score obtained by telephone. Statistics was performed using life-table methods.
Results: 33 patients were excluded from the research because of prestroke dementia. Poststroke dementia was diagnosed in 14.2% of cases at the end of the follow-up period, with most of PCI occurring during the first 6 months. Using multivariate analysis, independent predictors of PCI were found out: aging, preexisting cognitive decline, severity of deficit at the admission, diabetes mellitus, and silent infarcts. The presumed etiology of dementia was vascular dementia (VaD) in two-thirds of the patients, and Alzheimer’s disease (AD) - in one-third.
Conclusions: The risk of PCI is high, and it increases in patients with prestroke cognitive decline (about one-third of patients meeting the criteria for AD and two-thirds meeting the criteria for VaD). Treatment of poststroke dementia must be differentiated according to its heterotropic nature. Betropil - a medication with multimodal action (antioxidant, cyto- and neuroprotector) - is recommended (80-120 mg/day) to this category of patients.
13. Avagyan T., Varzhapetyan A., Barseghyan H.
Bovine pericardium as a possible transplant for the bile
J. Experimental and Clinical Medicine, 2011, 2, pp.64-70
Yerevan State Medical University,” Malatia” Medical Centre, Armenia
Bovine pericardium can be used for a number of applications. In this study, we evaluated its efficacy as a graft in a rabbit model bile duct surgery. The bovine pericardium was placed on the gallbladder window defect with subsequent histological and morphofunctional evaluation analysis of the gall bladder wall and prosthetic material in the 7, 14 days, three and six months. The analysis showed that in early stages (on the 7-th and 14-th days) the surface of the recovered material was covered with a monolayer of mesothelial-like cells. In addition, there was an infiltration of host cells into the matrix of the product, which suggests a replacement of the material with host tissue. Complete epithelization and replacement of the bovine pericardium patch with host tissue are described in late stages (in three and six months).
14. PETRIASHVILI G., CHIKOVANI M., MIRTSKHULAVA S., SALADZE T., TSINTSADZE T.
New technologies in the treatment of cataract
J. Experimental and Clinical Medicine, 2011, 2, pp.70-71
“Aversi” Clinic, Tbilisi, Georgia
With the development of phacoemulsification a new era has begun in cataract surgery. 3 types of lenses are used: 1. Monofocal, 2. Toric, and 3. Multifocal. Based on the worldwide statistical data, 15-20% of patients with cataract also have astigmatism in 1.5 (and more) dioptre. Owing to the new technologies for the correction of astigmatism, it is possible to use toric lenses during phacoemulsification. To avoid presbiopic eyeglasses, many patients prefer “monovision” correction: one eye is used for far vision and another one - for near vision. The procedure is more effective in patients aged 45-60.
15. Joglidze N., Mikeladze D., ZHuravlIova H., Sanikidze T.
Antialcochol Activity of HERBAL extract “Solani”
J. Experimental and Clinical Medicine, 2011, 2, pp.71-73
TSMU Institute of Medical Biotechnology, Tbilisi, Georgia
The antialcohol activity of the herbal extract “Solani” has been studied. The investigation results revealed that “Solani” induces an increasing activity of ALDH and ADH. The results make it possible to recommend “Solani” for the prevention of alcoholic intoxication and as a hepatoprotector.
16. DAVITASHVILI M.
INDUCING ACTION OF DICARBONIC ACID SALTS ON THE PRODUCTION OF
g-INTERFERON BY HUMAN, PORCINE, AND COW LEUKOCYTES
J. Experimental and Clinical Medicine, 2011, 2, pp.73-74
We have analysed the inducing effect of various re-activators containing Sodium, Potassium and Calcium dicarbonic acid salts on the production of g-interferon by human, porcine, and cow leukocytes. Limited re-activator mixtures were introduced into the growth medium for interferon biosynthesis so that the eventual concentration of dicarbonic acid salts would not exceed 0.08%. The increase in titres was noticed in the tests, to which the re-activators containing dicarbonic acid salts had been added.