2011. ¹ 4.




IMPROVEMENT IN Glaucine Hydrochloride tablets technology

J. Experimental and Clinical Medicine, 2011, 4, pp.8-14


1Tbilisi State Medical University; 2TSMU O.Kutateladze Institute of Pharmacochemistry, Georgia


Glaucine Hydrochloride is a drug formed of isoquinoline alkaloid-containing individual compounds. The goal of our research was to develop a resource-saving, cost-effective technology for Glaucine Hydrochloride tablets. Six different formulas of Glaucine had been prepared to have the optimal technological parameters. The normal ranges of humidity were outlined -1.5-3.0%, the optimal compression was defined as 120mp. The tablets prepared according to the G4 formula showed the best technological parameters.

Kinetics of the alkaloids release from Glaucine Hydrochloride and Glauvent was investigated via the rotary basket in a comparative aspect. 90% Glaucine Hydrchloride was released from the tablets in acidic medium in 180 minutes, at the rotational speed 100rt/min. For Glauvent tablets 90% of active ingredient release occurs in 90 minutes in the same conditions. In the tablets of Glaucine Hydrochloride prepared with the use of developed technology and composition the release of active ingredient is prolonged twice.




2.Topuria T., GHlonti S., Lolomadze Z., khodeli T., Chumburidze A., Jakeli K.

Immunological homeostasis IN children with pneumonia associated with Chlamydia 

J. Experimental and Clinical Medicine, 2011, 4, pp.14-17


G. Zhvania Pediatric Clinic; Institute of Medical Biotechnology, TSMU, Tbilisi, Georgia


The goal of the research was to study immunological homeostasis in children with pneumonia associated with Chlamydia. 

The immune status of 65 children less than 1 year of age was studied. Group 1 included 40 children with Chlamydia-associated pneumonia while Group 2 (control) consisted of 25 children with bacterial pneumonia. 

Based on the analysis of clinical observation and investigation conducted during the study, we concluded that Chlamydia-associated pneumonia in children of early age is indicated by the presence of mild and severe forms of respiratory failure, obstructive syndrome, tendency for prolonged inflammation, and development of immunodeficiency. Complex therapy failed to correct immunological disorders expressed in the children with Chlamydia-associated pneumonia. Taking into consideration ineffectiveness of complex antibacterial therapy used for the treatment of Chlamydia-associated pneumonia, we consider reasonable to involve immune-correcting therapy in the treatment of the disease to support normalization of homeostasis and to achieve a positive effect on the course of the disease.


3. Gongadze M., Sepashvili A., Gogia N., Atamashvili Ts., Mesropyan I., Iobadze M.

THE Influence of diabetes mellitus on IL-10 and TNF-α production by THE placenta

J. Experimental and Clinical Medicine, 2011, 4, pp.17-22


TSMU Institute of Medical Biotechnology; State Medical University;

 E. Andronikashvili Institute of Physics, Tbilisi, Georgia


Diabetes Mellitus in a pregnant mother can cause reproductive abnormalities, abortion, congenital anomalies, alterations of fetal growth and neonatal morbidity and mortality. The maternal diabetic environment alters the embryo and the feto-placental development. The results of these alterations are: increased embryo resorption and malformation rates, placental dysfunction. Cellular and molecular events in the uterine environment that lead to successful blastocyst implantation require the endometrium to be ready for being implanted; they include modified functions and populations of leukocytes, cytokine activities, and growth factors. To evaluate the anti-inflammatory role of IL-10 in diabetic pregnancy we performed in vitro study influence of hyperglycemia on the trophoblast from IL10-null mutant C57Bl/6 (IL10-/-) and control (IL10+/+) mice. Summarizing the experimental results, we can conclude that IL-10 modulates resistance to inflammatory stimuli by downregulating expression of proinflammatory cytokine TNF-α.




electron-microscopic changes of The loose connective tissue cells under the experimental prolonged squeezING

J. Experimental and Clinical Medicine, 2011, 4, pp.22-25



Crush injuries and crush syndrome are common after natural (e.g. earthquake, land-slide, tornadoes, and tsunami) or man-made catastrophes (e.g. wars, terrorist attacks); in fact the history of this disease is well reported both in earthquake rescue reviews and in military literature.

Intracellular changes of the loose connective tissue cells have been revealed under the electronic microscope during the experimental prolonged squeezing. The investigations resulted in the destruction of fixed cells of the loose connective tissue, leading to the chemical changes in the intercellular substance and functional failure of the mentioned tissue.




HIstochemical and hIstoenzimOchemical changes in hepatocYtES DURING tetrachlorOmetHanE-INDUCED toxic hepatitis

J. Experimental and Clinical Medicine, 2011, 4, pp.25-27


Department of Normal morphology, Tbilisi Medical Teaching University ,,HIPPOCRATES“, Georgia


The aim of our experiments is to determine the activity of Gentral in the liver tissue during acute hepatitis and its histochemical, histoenzimochemical and morphological effects. The experiments have been performed on 50 white rats which were injected in the area of back with CCl4 and 50% solution with 0.1 ml olive oils 5 times daily for 7 days. Our experiment showed that in the intact white rats liver acid phosphatase activity is of a moderate degree. In hepatocytes enzymatic activity is distributed evenly in the lobules and in those lymphocytes which infiltrate the liver portal tracts. Alkaline phosphatase is active in the sinusoidal walls, and in case of acute hepatitis becomes very negative, showing the lowering of transcapillary exchange in the liver parenchima. The use of Gentral (in comparison with the control group) maintains the amount of glycogen and increases the activity of alkaline phosphatase in the liver sinusoids.

It’s alleged that in the liver Gentral is transformed into homocystein and represents a great risk factor for the heart vessels and promotes the development of chemical pathology.



6.Tsetskhladze D., Chomakhashvili Z., Zosidze N., BolKvadze R.


J. Experimental and Clinical Medicine, 2011, 4, pp.24-33


Shota Rustaveli State University, Central Clinic, Batumi, Georgia


This investigation demonstrates that negative cholecystography can conceal important clinical information which in its turn extends the possibility of peroral cholecystography as one of the research methods and proves that its practical importance is not diminished. The origin of the negative peroral cholecystography can be grounded on different functional and organic disorders which may complicate the process of extravasation of contrasting substance into the gall-bladder.

After 3.5-4 hours of peroral administration of contrasting substance, Iodine absolute quantity double-increases and indicates the hepatobiliary system pathology or in other case - the alimentary canal pathology. Diagnostic algorithm, elaborated by the authors, is given in this work.





J. Experimental and Clinical Medicine, 2011, 4, pp.33-36


O.Gudushauri National Medical Center, Tbilisi, Georgia


The work deals with the dermatoses of pregnancy. Three clinical forms of dermatosis occurring during pregnancy (gestational pemphigoid, polymorphic eruption of pregnancy, and folliculitis of pregnancy) and the frequency of their occurrence have been studied in the perinatal department at NMC during 2008-2010. It is estimated that the frequency is equal to 3.5%. There is no difference between the frequencies of some clinical forms according to the years.




8. Znamenskaya T., Pokhilko V., Kovaleva E.,

Goncharova Y., Mironenko K.

medical/psychological ATTENDANCE FOR mothers HAVING THEIR INFANTS TREATED AT THE neonatal clinic

J. Experimental and Clinical Medicine, 2011, 4, pp.36-38


Ukrainian Institute of Pediatry, Obstetrics, and Gynecology, State University, Kiev; Ukrainian Medical Dental Academy, Poltava; Regional Children’s Hospital, Poltava, Ukraine



9. Vashadze SH.


J. Experimental and Clinical Medicine, 2011, 4, pp.38-40


 Shota Rustaveli State University, Batumi, Georgia


Our aim is to study the cases of cerebral blood flow disorder registered in Ajara for 2008-2010, to analyse them according to the risk factors, localization, and etiology for further prevention. 1405 cases of stroke were fixed in Ajara in 2008-2010: 53% of hemorrhagic stroke and 47% of ischemic stroke; 70% of patients are men and 90% of them are married. High risk groups consist of men aged 40 to 80 who underwent hemorrhagic stroke. It is necessary to carry out preventive measures in order to avoid stroke. High risk groups should be timely discovered. It’s also important to activate the sanitary instructive measures.




10. kachkachishvili I., korsantia b., Labartkava D.

A STUDY OF THE ImmunotropIC effectS of Kamelin IN volunteers

J. Experimental and Clinical Medicine, 2011, 4, pp.40-42


Ltd “Mitra” Dental Clinic, Doctoral Candidate of Tbilisi State Medical University;

Institute of Medical Biotechnology, TSMU, Georgia


On the example of the interferon system, it has been shown that Kamelin caused a moderate increase of endogenous IFN-g activity (about 31.2 up to 41.6 U/ml). The greatest effect was manifested in patients with low initial levels of interferon. Kamelin is considered to be of wide use in clinical practice.




11. Vashadze SH.

DIAGNOSTIC criteria foR Anorexia nervosa

J. Experimental and Clinical Medicine, 2011, 4, pp.38-40


Shota Rustaveli State University, Batumi, Georgia


Anorexia nervosa is an eating disorder characterized by refusal to maintain a healthy body weight, of Mental Health Disorders (DSM-IV), Published by The American Psychiatric Association. The DSM-IV should not be used by laypersons to diagnose themselves. DSM-IV-TR: diagnostic criteria for AN includes intense fear of gaining weight, a refusal to maintain body weight above 85% of the expected weight for a given age and height, and three consecutive missed periods and either refusal to admit the seriousness of the weight loss, or undue influence of shape or weight on one’s self image, or a disturbed experience in one’s shape or weight. There are two types: the binge-eating/purging type is characterized by overeating or purging, and the restricting type is not. Criticism of DSM-IV. There has been criticisms over various aspects of the diagnostic criteria utilized for anorexia nervosa in the DSM-IV. Including the requirement of maintaining a body weight below 85% of the expected weight and the requirement of amenorrhea for diagnosis; some women have all the symptoms of AN and continue to menstruate. Those who do not meet these criteria are usually classified as eating disorder not otherwise specified this may affect treatment options and insurance reimbursements. The validity of the AN subtype classification has also been questioned due to the considerable diagnostic overlap between the binge eating/purging type and the restricting type and the propensity of the patient to switch between the two. ICD-10: The criteria are similar, but in addition, specifically    mention. The initial diagnosis should be made by a competent medical professional. Detailed physical examination and laboratory screening are indicated as a routine procedure in the initial evaluation of psychiatric patients. Most patients are unaware of the medical illness that is causative of their psychiatric symptoms. The conditions of patients with medically induced symptoms are often initially misdiagnosed as a functional psychosis. There are a variety of tests that may aid in the diagnosis of AN and the assessment of possible secondary effects caused by AN upon the patient. There are various medical and psychological conditions that have been misdiagnosed as anorexia nervosa, in some cases the correct diagnosis was not made for more than ten years. In a reported case of achalasia misdiagnosed as AN, the patient spent two months confined to a psychiatric hospital. There are various other psychological issues that may factor into anorexia nervosa, some fulfil the criteria for a separate Axis I diagnosis or a personality disorder which is coded Axis II and thus are considered comorbid to the diagnosed eating   disorder. Axis II disorders are subtyped into 3 “clusters”, A, B and C. The causality between personality disorders and eating disorders has yet to be fully established. Some people have a previous disorder which may increase their vulnerability to developing an eating disorder Some develop them  afterwards The severity and type of eating disorder symptoms have been shown to affect comorbidity.





J. Experimental and Clinical Medicine, 2011, 4, pp.44-45


Institute of Postgraduate Medical Education and Continuous Professional Development, TSMU, Tbilisi, Georgia



13. T. Mchedluri, M. Metreveli, M. Nikolaishvili

Eco-toxicological plural of  basin river “Alazani”

J. Experimental and Clinical Medicine, 2011, 4, pp.45-48


Telavi Stade University, Georgia


Studies that were conducted in 2009-2010 in the river basin Alazani, showed that the amount of soluble heavy metals, iron, mercury, copper, potassium, lead and manganese in the water did not exceed the maximum permissible concentration. Alazani water use for irrigation (vineyards) in the region, which has a detrimental effect on the biosystem Alazani. Despite this, the condition of the river can be considered good enough.