J.EXPERIMENTAL & CLINICAL MEDICINE
2013, ¹ 4
1. TODUA P., ORMOTSADZE G., NADAREISHVILI D., CHELIDZE L.
LIFETIME END AGE-CONDITIONAL CANCER RISK IN TBILISI POPULATION
J. Experimental and Clinical Medicine, 2013, 4, pp.10-13
Ivane Beritashvili Center of Experimental Biomedicine; Department of Nuclear and Radiation Safety of Ministry of Environment Protection and Natural Resources, Tbilisi, Georgia
Lifetime end age-conditional cancer Risk in Tbilisi population has been estimated by the competing risk of mathematical methods. The mentioned characteristic is a basic component of age- and gender-dependent radiogenic risk models, which is a main instrument for retrospective radiation risk analyses according to 103 and 105 recommendations of International Commission on Radiological protection.
There was shown that lifetime cancer risk in Tbilisi population equals 0.22 which is about two-fold less than the similar rate of the USA and some European countries. If we consider the current findings on anti-correlation between spontaneous and radiogenic cancer risks, we think that detailed study of the mentioned matter will be reasonable in consideration of radiation safety of Georgian population.
2. Okropiridze T., Pirtskhalava M., Kapanadze R., Motsonelidze N.
Experimental usage of plasmatic irradiation for osteogenesis stimulation
J. Experimental and Clinical Medicine, 2013, 4, pp.13-15
University “Geomedi”, Department of Stomatology, Tbilisi, Georgia
The work represents experimental and morphological studies of regeneration of damaged areas of maxilla-facial bones under the influence of the plasmatic stream. During the treatment we used micro plasmatic scalpel-irradiator “ARIELI XXI”. The therapy showed positive results.Thus we recommend this method of treatment for introducing in practice.
3. Pavliashvili N., Kipiani V., Petriashvili T., Topuridze M., Kipiani N., gamkrelidze N.
Changes of regional blood circulation and microhemocirculation at inflammation in the animals with Brown-Pears carcinoma
J. Experimental and Clinical Medicine, 2013, 4, pp.16-18
Department of Pathophisiology, TSMU, Georgia
With the use of hydrogen clearance method in the animals with Brown-Pears carcinoma we investigated the intensity of blood flow (IBF) in a rabbit ear at inflammation that was induced by Samuel’s classical method.
It was shown, that IBF changes as in inflammation area as well as in “perifocal” zones in cancer animals compared with intact ones progresses on lower baseline level. Analogical type of IBF changes are also revealed at pharmacological desimpatization of rabbit ear that indicates that based on our results and also according to our further data, mechanism of extrafocal changes is being realized by humoral ways. We can conclude that IBF decrease at paraneoplastic condition is the universal phenomenon, which is characteristic to tumorous growth generally and is caused by peroxidative impairment of vascular wall.
4. Shavdia M.D., Gvamichava R.R., Giorgobiani L.Z.
The assessment of Gabapentin analgesic effect, in residual pains, under conditions of tumorous lesions of the bone tissue
J. Experimental and Clinical Medicine, 2013, 4, pp.19-22
Tbilisi State Medical Universitate and Cancer Prevention Centre, Georgia
The assessment of Gabapentin analgesic effect, in residual pains, under conditions of tumorous lesions of the bone tissue. 73 patients with advanced cancer, were involved in the research – A (main-38) and B (control-35) groups. The mean point of pain in the patients who participated in the research was 6,5 by VAS, and despite analgesic therapy there still was residual pain, 2,8 by VAS. Methods: All patients from group A received Gabapentin: I day – 21.00 300mg; II – 9.00 and 21.00 300mg; III – 9.00, 15.00 and 21.00 300mg; This regimen was during 15 days. The patients from group B didn’t received Gabapentin. The assessment of patients health was made by KPS, ECOG, VAS and questionnaire, which was divided on I-XV days. Results: In the group A the point of residual pain, after the research, reduced from 2,8 to 0,9, by 1,9 balls (P<0,05). In addition, from 28 patients who had mild pain, in the end of the research 10 patients had no pain, from 10 patients with moderate pain 2 had no pain, and 8 had mild pain. Only in 4 patients the point of pain didn’t change. In the group B the point of residual pain, after the research, reduced from 3,0 to 2,6, by 0,4 balls. In patients of the group A the results of KPS increased from 31,8% to 38,9%, and the results of ECOG increased in 19 patients (50%, P<0,05). Also, in the group B, the results of KPS decreased from 32,9% to 28,6%, and ECOG results decreased in 12 patients (34,3%, P<0,05). In the end of the research, in the group A, from 35 patients 15 (42.9%) had insomnia. From that, 8 had mild insomnia, 5 – moderate, 2 – severe. So, from 35 patients with insomnia, 31 (88,6%, P<0,05) were relieved. From 10 patients with mild insomnia, none of them had insomnia in the end of the research; From 16 patients with moderate insomnia 9 were relived, 5 had mild insomnia, and only 2 stayed the same. In the end of the research, in the group B, from 30 patients 25 (83,3%) had insomnia and only 5 were relieved. In patients of the group A the results of KPS increased from 31,8% to 38,9%, in the group B - decreased from 32,9% to 28,6%. Conclusion: Gabapentin reduces the residual pain up to 2 points by VAS. In case of residual pain, caused by tumorous lesions of bone tissue, use Gabapentin only as coanalgetic.
5. I.ABESADZE, R.GVAMICHAVA, F.DZOTSENIDZE, M. SHAVDIA, L.GIORGOBIANI,
G. METIVISHVILI, T. LETODIANI, E. ABZIANIDZE
CHRONIC PAIN MANAGEMENT – CHALLENGES AND THE WAYS OF SOLUTION
J. Experimental and Clinical Medicine, 2013, 4, pp.23-26
Cancer Prevention Center; Tbilisi state Medical University; The Institute for Cancer Prevention and Palliative Medicine, Tbilisi, Georgia
Quality of pain management highly depends on access to opioids and existence of relevant legislation regulating the rules of prescribing and administration of these medications. Assessment of the National Policy on Opioids, basing on WHO document “Achieving Balance In National Opioids Control Policy“ performed by the state authorities in close cooperation with Palliative Care team, have resulted in legislative changes, focusing on all angles of WHO triangle for adequate pain management: Policy, Drug Availability and Education.
Goals: Introduction of the State Policy on Quality Pain Management and legislative changes undertaken in Georgia since 2006.
The “Law on Regulation of Legal Use of Narcotics” (2007) - commitment undertaken by the State for provision of patients with necessity of opioids both in terms of the quantity and forms;
Paragraph concerning inevitability of opioid usage for medical reasons was added to the Chapter, reflecting the main principles and regulation field (2012).
The language was refined and confusing terminology and definitions were removed (2012):
· Introduced new definition of dependence “physical dependence”-as sole criteria for dependence diagnose is removed.
· Stigmatizing term “somatonarcoman” (patient with physical dependence) is removed;
· State Health Program from 2011 provides opioids for noncancerous patients, alongside with cancer patients.
Increasing of opioid supply from 3 to 7 days (2008); Instead of the commission for prescribing the opioids, prescription became one authorized physicians responsibility; Adoption of a new prescription form (2010) - two different kinds or formulations can be prescribed; Authorization on prescription of opioids was passed to PCP-s.
Results: Quality pain management became priority of State policy; new legislations ensure balance for medical usage of opioids and preventive measures for diversion; Different formulations and dosage of opioids became available in Georgia since 2008.
EPIDEMIOLOGY OF MALIGNANT TUMORS IN GEORGIA AND ECOLOGICAL FACTORS
J. Experimental and Clinical Medicine, 2013, 4, pp.27-31
A.Gvamichava National Cancer Center, Tbilisi, Georgia
The epidemiology of malignant tumors is actively developed in last decade. Mathematical-statistical methods of forecasting of morbidity and death-rate are elaborated. But in same time not infrequently there is lack of complex approach – with taking into consideration diversity of factors, taking part in ecological processes. The aim of this research study is of the epidemiology of malignant tumors in Georgia, ecological factors and their influence on the spreading of malignant tumors. The method of “superimposed epochs” was used.
As the result of the research it is ascertain, that by regions less hazardous are Svaneti and Racha-Lechkhumi, the most hazardous – sea coast of the Black Sea and Kartli. The solar activity and value of ozone layer determine elevation of cancer morbidity incidence. Obtained results are of great importance for revealing of cancer “risk-factors” and, besides the scientific, are of great economical importance for optimal distribution of expenditures for measures against cancer.
7. GONGADZE N., KEZELI T., MIRSIASHVILI M., MAKHARADZE T., ABULASHVILI D., GOGOLAURI M.
THERAPEUTIC TARGETS FOR PREVENTION AND TREATMENT THE PROGRESSION OF DILATED CARDIOMIOPATHY
J. Experimental and Clinical Medicine, 2013, 4, pp.31-34
Tbilisi State Medical University – Departments of Pharmacology and Therapy; Iv.Javakhishvili Tbilisi State University – Department of Pharmacology; Department of Anaesthesiology of Tbilisi ¹1th Hospital, Georgia
8. ANTELAVA N.A., GHONGHADZE M.V., OKUJAVA M.V., PACHKORIA K.Z., GVISHIANI M.V.
EPIDEMIOLOGY OF DRUG INDUCED DAMAGE OF LIVER CAUSED BY ANTIBACTERIAL PREPARATIONS AND WAYS OF PREVENTION OF HEPATOTOXICITY
J. Experimental and Clinical Medicine, 2013, 4, pp.35-40
Tbilisi State Medical University, Georgia
In the article we reviewed the existing data concerning prevalence of drug induced damage of liver (DIDL), caused by the antibacterial preparations and ways of prevention of subsequent hepatotoxicity. It is intended that DIDL caused by antibacterial drugs arises in 1 from 5000-10000 patients. In USA 45,5% of all DIDL is induced by antibacterial preparations and leading place among reasons belongs to widely used drugs like antituberculotic, amoxicillin/ clavulonic acid and Flucloxacillin. In the review are presented data of epidemiology related to main groups of antibacterial preparations (penicillins, cephalosporins, microlides, tetracyclines, fluoroquinolones, metronidazol and antituberculotics). The considerable increase of DIDL is caused by liberalization of drug’s achievement for consumers, disregard of drug administration rules and regimens, prevalence of other chronic diffuse diseases of liver among population and with pollution of the environment. The genetic and nongenetic risk-factors of DIDL and main ways of prevention are discussed in the article.
9. Tchumburidze T., Isakadze A., Nemsitsveridze N., Guraspashvili A., chikviladze T., Gokadze S.
Collaboration practice between clinicist and pharmacists
J. Experimental and Clinical Medicine, 2013, 4, pp.40-42
TSMU Department of Social and Clinical Pharmacy, Tbilisi, Georgia
Collaboration between healthcare professionals is the heart of any good clinical practice. Multi-skilling and task-shifting are increasingly viewed as important ways to manage the critical shortages of health care workers in many parts of the world.
The degree to which pharmacists collaborate with physicians varies both across healthcare systems, but also within the same healthcare system. The level of collaboration between pharmacists and other healthcare professionals goes from minimal contact through to pharmacists who are seen as a core part of the multi-disciplinary team with the authority to initiate and modify medicine therapy. In more advanced practice settings termed “Collaborative Pharmacy Practice” (CPP) the pharmacist is recognized by the multidisciplinary team as the lead professional in managing patients medication therapy.
10. NEMSITSVERIDZE N., TCHUMBURIDZE T., PERADZE N., DUGASHVILI N., ZARKUA T., ERIASHVILI V.
FEATURES OF MANAGINGPHARMACEUTICAL STUFF
J. Experimental and Clinical Medicine, 2013, 4, pp.42-43
TSMU Department of Social and clinical Pharmacy
Management of the stuff is the major task of any company in modern business. The purpose of our research was studying features of management of stuff in the pharmaceutical companies. Information is received by questioned poll of 50 specialists from various pharmaceutical companies of Georgia. Poll was made anonymously, respondents only specified a work place. The obtained data allows to create general idea about personnel management of pharmaceutical companies in Georgia. Shortcomings of organizational character connected with operating time excess, violation of procedure while signing the contract, and also an authority abuse and insufficient attention to introduction of clinical pharmacists in drugstores and the clinics, insufficient motivation to increase of level of pharmaceutical education are revealed.
11. KVIZHINADZE N.
DEFINITION OF PHARMACOECONOMICS AND PERSPECTIVES IN DEVELOPING COUNTRIES
J. Experimental and Clinical Medicine, 2013, 4, pp.43-45
Department of Social and Clinical Pharmacy, Tbilisi State Medical University, Georgia
A pharmacoeconomic study evaluates the cost (expressed in monetary terms) and effects (expressed in terms of monetary value, efficacy or enhanced quality of life) of a pharmaceutical product. There are several types of pharmacoeconomic evaluation: cost-minimization analysis, cost-benefit analysis, cost-effectiveness analysis and cost-utility analysis. Pharmacoeconomic studies serve to guide optimal healthcare resource allocation, in a standardized and scientifically grounded manner. The aim of the study is to maximize treatment outcomes within limited budgets. Due to the high alternative costs of scarce funds, it may be argued that pharmacoeconomics is relatively more important in developing countries than in developed countries
12. KVIZHINADZE N., MIRVELASHVILI E.
THE ROLE OF PHARMACIST IN HIGH SCHOOLS
J. Experimental and Clinical Medicine, 2013, 4, pp.45-47
Tbilisi State Medical University – Department of Clinical and Social Pharmacy;
Department of Public Health, Georgia
Pharmacists can play a potentially important role in improving medication management in schools. Pharmacists are likely to interact with prescribers, school personnel, parents, and possibly with the children, themselves. Unfortunately, little research has been done regarding the pharmacists’ role in medication management in high schools. Since there will always be children that need to take medication at high school, it is important that pharmacists be aware of implications surrounding medication management. The population who responded to our survey overwhelmingly thought this was an important issue; however, there was relatively little involvement in this area in terms of research, teaching, and service. There is an immediate need to better understand and improve medication management in schools, so perhaps more professionals will turn their attention to this important area of drug therapy in the future.
13. NIKURADZE N.
PHARMACOEPIDEMIOLOGICAL RESEARCH MONITORING
J. Experimental and Clinical Medicine, 2013, 4, pp.47-48
Tbilisi State Medical University, Chair of Social and Clinical Pharmacy, Georgia
Monitoring – new pharmacoepidemiological research method. Monitoring of Tbilisi chemist’s-shops shared the following statistics: Sleeping pills, Antiepileptic drugs, Myorelaxants, some of the cough medicines – shoud not be sold without medical prescription. Antiepileptic drugs: Grimodin, Tebaltin, Roalebtin, Gabagamma Sleeping pills: Somnol, Imovan, Drimolin, Andante; Myorelaxants: baclosan, Baclofen, Phinlepsin; Cough medicines: Magituss, Tussin plus – are often taken for their pleasant effects, that also give some narcotic effects or euphoria; be prescribed and sold for medical purposes. In Georgia it demands strict control.
14. GOKADZE S., MULKIJANYAN K., BARBAKADZE V., MIKAIA G., KURDIANI N., BAKURIDZE A.
ELABORATION OF WOUND HEALING OINTMENT BASED ON PHENOLIC POLYMER FROM STEMS OF SYMPHYTUM ASPERUM
J. Experimental and Clinical Medicine, 2013, 4, pp.49-52
Tbilisi State Medical University, Faculty of Pharmacy; I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
The results of the development of wound healing ointment based on phenolic polymer from comfrey PDGA are presented. The optimal ointment formulation has been justified on the basis of experimental studies carried out.
The colloidal stability of PDGA containing ointment has been studied. The ointment remains stable both right after manufacturing and during storage at different temperatures for 24 hours with following centrifugation.
Biopharmaceutical studies established that the maximal release of PDGA from the ointment was achieved when sodium alginatis was used for an ointment base. Animal studies revealed that the ointment exhibits pronounced wound healing action.
15. SHASHIASHVILI N., MURTAZASHVILI T., BAKURIDZE L., TSURTSUMIA I., TSAGAREISHVILI N., BAKURIDZE A., BERASHVILI D.
SCREENING OF SOME LAMIACEAE PLANTS OF GEORGIA FOR ANTIOXIDANT ACTIVITY
J. Experimental and Clinical Medicine, 2013, 4, pp.53-56
Tbilisi State Medical Univesrity, Department of Pharmacognosy and Botany, Georgia
The antioxidant activities of Lamiaceae plants, have been determined by using 1,1-diphenyl-2-picrylhydrazyl (DPPH). All extracts of 10 species were shown to possess a scavenger activity against DPPH free radical. Considerable radical scavenging and higher antioxidant activities were determined by the extracts of Perilla nankinensis, and Origanum vulgare, Thymus vulgaris.
Rosmarinic acid (RA) is a phenolic compound which is found in many genus of Labiatae and exhibits important biological activities. It is a natural phenol antioxidant. We have studied RA contents of the plants by using spectrophotometric method. The results showed that RA content in different species of Labiatae was 0.69-4.19% of dried plants. The highest amount of RA was found in Origanum vulgare, Salvia officinalis, Melissa officinalis species. The result of correlation analysis indicated that rosmarinic acid was the major contributor to the antioxidant activities. Although plant extracts contain different bioactive compounds that deactivate free radicals through a multilevel cascade approach. So, antioxidative activity is a synergistic action of the plant compounds.
16. ZARDIASHVILI L., KUCHUKHIDZE J., JOKHADZE M., MSHVILDADZE V.
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF
FLAVONOIDS IN GEORGIAN ENDEMIC BETULA MEGRELICA LEAVES
J. Experimental and Clinical Medicine, 2013, 4, pp.56-58
Department of Pharmacognosy and Botanica TSMU, Georgia
There is carried out the qualitative-quantitative analysis of flavonoids of the leaves of birch Betula Megrelica the Georgian endemic plant, with the liquid chromatographic method.
As a result of the experiment we have estimated the optimal conditions of the chromatography: the gradient mode of the moving phase, the stationary phase and the detection wavelength in the ultraviolet region, we have estimated that the leaves of Betula Megrelica contains the following flavonoids (mg/g): Hyperozide (4,6), Myricetin (2,1), Naringenin (1,7), Kaempferol (2,7), Quercetin (3,5), Rutin (3,7), Methoxyluteolin (1,6), Catechin (2,5). Thus, Georgian endemic species Betula Megrelica is rich with flavonoids units and represents an interesting object for further deep scientific study.
17. MURTAZASHVILI T., BAKHTADZE N., NOZADZE B., SIVSIVADZE K., CHIKVILADZE T., CHINCHARADZE D.
Validation of the method for determining qualitative and quantitative characters of Furfurol via high-performance liquid chromatography in the drug “Cameline-M1”
J. Experimental and Clinical Medicine, 2013, 4, pp.58-61
Tbilisi State Medical University, Georgia
In the article it is discussed High-performance liquid chromatography for determining qualitative and quantitative characters of Furfurol, which can be used for the standartization of “Cameline M1”. The method is validated, the results are worked out mathematically. Method selectivity, system validity compliance, robustness, linearity, accuracy, specificity, analytical method usage range, detection and quatitation limits have been studied.
18. Kunchulia L., Murtazashvili T., Imnadze N., Jokhadze M., Nozadze B., Lekishvili N.
Study of Antioxidant Activity of Propolis Extracts Received with Various Methods of Extraction
J. Experimental and Clinical Medicine, 2013, 4, pp.62-63
TSMU, Department of Pharmaceutical and Toxicological Chemistry;
TSMU, Department of Pharmacognosy and Botanics, Tbilisi, Georgia
Propolis – is the product of the bees, which are used as a medicine from ancient period. The aim of our study was to determine the antioxidant activity of extracts of this product, obtained with various methods of extraction. The free radical capturing ability of the propolis was studied under the method described by Yamaguch et al. (1998).
During the study was determined the in vitro antioxidant activity of the extracts received from Propolis of Kartli region. The sample of cold water extraction is characterized with impression value of antioxidant activity. So called aquapropolis received from Georgian propolis, is the product with high potential ability. It could be utilized not only as a medicine, as well the cosmetic agent.
19. Imnadze N., Gurgenidze I., Makharadze R., Nizharadze N.
Polarographic Method of Analysis of Tramadol
J. Experimental and Clinical Medicine, 2013, 4, pp.64-67
TSMU, Department of Pharmaceutical and Toxicological Chemistry;
R. Agladze Institute of Inorganic Chemistry and Electochemistry, Tbilisi, Georgia
The development of sensitive and specific methods of analysis of narcotic and psychotropic agents is the main subject of study of present day pharmacy, narcology and toxicology.
During of this study was developed the optimal conditions of polarographic recovery of tramadol. By this method, it is possible qualitative and quantitative determination of tramadol in following frames 1×10-6-1×10-2M. Was studied absorptive nature of tramadol by impedance method of analysis. Developed polarographic method gives an opportunity to determine tramadol as qualitatively as quantitatively even in minimal quantities in subject of research.
20. Adeishvili-Andghuladze L., Jokhadze M., Makharadze R., Gonashvili M., Nozadze B.
Development of Gas Chromatography-Mass-Spectrometric (GC-MS) Method of Determination of Methadone in Biological Fluids
J. Experimental and Clinical Medicine, 2013, 4, pp.67-69
TSSU, Department of Parmaceutical and Toxicological Chemistry; Department of Pharmacognosy and Botanic; Department of Forensic medicine; Tbilisi, Georgia.
To confirm the positive results of the screening test, in chemichal - toxicological analysis used the method of Gas Chromatography-Mass Spectrometry. It is considered as one of the most exact method of analysis of chemichal substances. Respectively utilization of this method in determination of widely spread narcotic drug-methamphetamin in biological fluids is important task.
As a result of experimental work, was determined optimal conditions of analysis: temperature of injector, fuel and transferline, the thermal gradient, volume of injection, length of the column, thickness of the layer, gass-wearing speed of mobile phase.
Detection of methamphetamine was carried by TIC regime, under the NIST Data Base. For derivatisation was selected acitilation (acetate anhydride), metilation and sylitation were performed by – MSFTA; BSTFA; MSTSA+1%TMCS; BSTFA+1%TMCS)
The optimal silitation of methamphetamine was performed by BSTFA. In the current is presented the chromatograme of methamphetamine isolated from blood and urine, the mass-specter of methamphetamine detected in blood and bibliography, as well the referal mass-specter of methamphetamine under NIST - database.
21. ROVIELLO G., PIRTSKHALAVA M., EGOYAN A., MIRTSKHULAVA M.,
THE SYNTHESIS OF A CHIRAL NUCLEOPEPTIDE FOR BIOMEDICAL APPLICATIONS
J. Experimental and Clinical Medicine, 2013, 4, pp.70-73
Institute of Biostructure and Bioimaging of the Italian National Research Council, Naples, Italy
University “Geomedi”, Tbilisi, Georgia
Chiral nucleopeptides are among other nucleo-amino acid-based compounds which are recently studied for their possible utilization in biotechnological and medical strategies. Their structural characteristics, as well as some properties of molecular recognition could be beneficial in the development of innovative drugs for anticancer and antiviral therapies. In the present research we present the results of experimental investigation regarding the synthesis of a chiral nucleopeptide as well as the study of its ability to bind a target such as poly rA RNA. Experiments were performed by Circular Dichroism (CD) under controlled pH and temperature conditions using concentrations of the ligand corresponding to those reported in literature for analogous cases. The dramatic change in CD signal that followed the mixing of the two solutions containing the thymine nucleopeptide and Poly rA clearly suggested the formation of nucleopeptide/RNA complex with a nucleobase T:A=2:1 ratio. Molecular modeling calculations also clearly demonstrated that the whole system tends to establish a complex structure due to the interactions between the RNA (A18) and nucleopeptide (T6) molecules.
22. PIRTSKHALAVA M., ROVIELLO G., EGOYAN A., MIRTSKHULAVA M., MOTSONELIDZE E.
USE OF COMPUTER MODELING OF CHIRAL NUCLEOPEPTIDES IN PHARMACY
J. Experimental and Clinical Medicine, 2013, 4, pp.73-75
University “Geomedi”, Tbilisi, Georgia
Institute of Biostructure and Bioimaging of the Italian National Research Council, Naples, Italy
Nucleopeptides are hybrid molecules which contain both amino acid and nucleobase moieties in their stuctures and often show interesting properties of molecular binding that render them interesting candidates for development of innovative drugs for anticancer and antiviral therapies. In this work we present the results of computer modeling regarding the ability of a chiral nucleopeptide to bind a target such as poly rA RNA. In our case modeling studies were performed by Hyperchem 8.0 program (Hypercube Inc., Gainsville, FL, a trial version) on nucleopeptide (T6) and A18 RNA. The study of the interaction between the nucleopeptide (T6) and RNA (A18) as well as the calculation of the binding energies were performed through the Amber MM method of molecular dynamics simulation using parameters corresponding to those described in literature for examples similar to ours and simulation temperature - 300 K. For geometry optimization Polak-Ribiere method with termination condition such as RMS gradient was used. Molecular modeling calculations clearly demonstrated that the whole system tends to establish a complex structure due to the interactions between the RNA (A18) and nucleopeptide (T6) molecules. Thus, binding energy of the complex assumes a negative value (-72.18 kcal/mol) as reported in this work.
THE OLEFINS METATHESIS – STRATEGY OF SYNTHESIS OF PHEROMONES AND OTHER NATURAL COMPOUNDS
J. Experimental and Clinical Medicine, 2013, 4, pp.76-80
Department of Medical Chemistry, Tbilisi State Medical University, Georgia
The olefins metathesis reaction is the effective mechanism of synthesis of different compounds, obtaining of those is quite problematical by using of classical approaches; via combination of Z-stereoselective and nonstereoselective modifications of cometathesis of COD and ethylene a mixture of (Z + E)-5-undecenoic acids (sex pheromone of Anghrenus verbasci) are produced.
24. TSITSAGI M., ARZIANI B., CHKHAIDZE M., KHACHIDZE M.
NATURAL FOOD COLORANTS AND ORGANIC FOOD PRODUCTION
J. Experimental and Clinical Medicine, 2013, 4, pp.80-82
Department of medical chemistry TSMU, Tbilisi, Georgia
Organic foods are foods that are produced using methods of organic farming. They do not involve modern synthetic inputs such as synthetic pesticides and chemical fertilizers. Organic foods are also not processed using irradiation, industrial solvents, and synthetic food additives. In order to replace synthetic food colorants by natural ones is the actual problem. β-carotene and lycopene are most abundant and used carotenoids which are approved as natural food colorants and can be used to color organic food.
Supercritical fluid extraction of these carotenoids from vegetable and fruit matrices could be an environmentally friendly alternative to the conventional solvent extraction usually used for receiving of these compounds.
25. Dzadzua J., Darsania T.
For dissociating medical terminology in Georgian
J. Experimental and Clinical Medicine, 2013, 4, pp.82-84
SSU Humanitarian Faculty; TSMU Nutritional and Age Medicine Direction, Tbilisi, Georgia
For the Russian medical terminology characteristic word «áîëåçíü» in the Georgian language correspond to three lexical units: avadmyofoba, sneuleba, daavadeba. The semantic identification of these synonyms is only a superficial impression. The overview of the dictionary materials of medical terms and special texts, shows that these lexical units selected the use of certain principles: ~avadmyofoba~ is used in such combinations, which represent specific “subspecialty” pathology. The semantic meaning of the word ~sneuleba~ is similar to the word ~~avadmyofoba~ and it occurs very rarely in modern medical literature. Similar derivatives are also found of the lexical unit ~daavadeba~. Lexical unit ~daavadebuli~ gradually acquires a particular value and in most cases is used to mean ~senSeyrili~ (çàðàæ¸ííûé). This in turn causes a semantic change in its root word ~daavadeba~, which is set as the term marked and separated from the lexical units ~sneuli~, ~avadmyofi~.
26. NAMORADZE M., KIPIANI V., CHELIDZE N., KATSADZE M.,
KARKARASHVILI D., KIPIANI NINO
POSSIBLE MECHANISMS OF MYOCARDIAL INJURY AT HYPERBARIC OXYGEN ACTION
J. Experimental and Clinical Medicine, 2013, 4, pp.85-86
Department of Pathophysiology, TSMU, Georgia
With the use of electronic paramagnetic resonance (EPR) method has been studied effects of hyperbaric oxygenation (HBO) on oxidative processes. Also using hydrogen clearance method (polarography) – was investigated intensity of local blood circulation (LBC) in myocardium. Experiments were carried out on 80 laboratory mice. The used regimens of HBO were: single 1 hour exposure of 2 and 3 Atmospheric pressure. Experiments revealed that both regimens of HBO cause decrease of intensity of LBC in myocardium, increases formation of free radicals, decreases activation of antioxidative ferments, increases peroxidation of lipids that lead to membrane structures disorders. The changes mentioned above are significantly expressed at 3 atmospheric pressure exposure.
27. GABUNIA L., KHETSURIANI SH., KHETSURIANI K., GAMBASHIDZE K., VADACHKORIA D.
MODERN ASPECTS OF RESEARCH ETHICS
J. Experimental and Clinical Medicine, 2013, 4, pp.87-89
Tbilisi State Medical University, Georgia