J.EXPERIMENTAL & CLINICAL MEDICINE
2014, ¹ 1
N. KILADZE, T. SHULAIA, N. KORSANTIA
NEW ALGORITHM’s DERMATOSCOPIC CRITERIA FOR DIAGNOSIS VERIFICATION
J. Experimental and Clinical Medicine, 2014, 2, pp.9-12
Dept of Dermatology and Veneorology TSMU; Medical Center “Marjani 21”, Tbilisi
Dermoscopy is a simple and non-invasive investigation technique which gives a possibility to see and estimate the morphological details of skin rashes and to establish the proper diagnosis. Is is especially important for diagnosis and differentiation of pigmented and unpigmented lesions. For correct application of this technique is needed the special training and knowledge of main dermoscopic criteria. The present article was aimed to describe and present for medical society the dermoscopic criteria of new algorithm, proposed by prof. Kittler which is consistent, comprehensive and accessible even for beginners.
I. Z. Gladchuk, O. Ya. Nazarenko
Peculiarities of laparoscopic operations in massive intraperitoneal hemorrhages in gynecology.
J. Experimental and Clinical Medicine, 2014, 2, pp. 12-15
Odessa National Medical University, Department of obstetrics and gynecology ¹1, Ukraina
We have carried out the retrospective analysis of 785 laparoscopic operations under the conditions of hemoperitoneum of different volume (from 50 to 2100 ml). The cause of hemoperitoneum in 511 cases was the disturbed tube pregnancy, and in 274 cases - apoplexy of the ovary. In 471 patient the volume of hemoperitoneum did not exceed 500 ml, 195 women were operated on under the conditions of moderate blood loss - from 500 to 1000 ml, in 79 women the volume of hemoperitoneum was from 1000 to 1500 ml and in 40 patients the operation was performed under the conditions of massive blood loss, more than 1500 ml. Laparoscopic operations under the conditions of moderate, large and massive intraperitoneal blood loss can be divided conditionally into three stages: resuscitative, haemostatic and sanitation. The crucial point of the resuscitative stage is reinfusion of the autoblood, which was performed employing the procedure proposed by the authors. The volume of the haemostatic stage of the operation was individual, but to a considerable degree it depended on the volume of hemoperitoneum. All operations were successfully performed laparoscopically without the intra-operative or postoperative complications. Laparoscopy allows to use resuscitative measures rapidly that are directed at blood replacement by the autoblood of the patient under the conditions of moderate, large and massive intraperitoneal blood loss, to establish the source of intraperitoneal hemorrhage and to perform adequate haemostatic operation.
Gvidani S., Sumbadze Ts., Kapanadze N., Kobidze T., Mumladze M.
THE REACTION OF CYCLIC NUCLEOTIDES ON THE INTOXICATION OF THE ORGANISM BY PHENOL VAPOR
J. Experimental and Clinical Medicine, 2014, 2, pp. 15-20
Javakhishvili Tbilisi State University; A. Natishvili Institute of Morphology, Tbilisi, Georgia
The dynamics of change and the mechanism of cyclic nucleotides during intoxication by phenol vapor are investigated. It is established, that after the inhalation of phenol vapor the ratio between cyclic adenosine-31,51-monophosphate and cyclic guanozine-31,51-monophosphate changes; in “lost” animals the said ratio increases at the expense of an increase in the adenosine-31,51-monophosphate amount, but in “survived” animals the ratio at first slightly decreases and further again returns to a reference value.
M. PKHALADZE, T. KVACHADZE, N. SAKVARELIDZE, E. NIKOBADZE
THE ESTIMATION OF THE CELL-STROMAL RATE IN THE GINGIVAL SUBEPITHELIAL CONNECTIVE TISSUE UNDER THE EXPERIMENTAL GINGIVITIS.
J. Experimental and Clinical Medicine, 2014, 2, pp. 20-24
Tbilisi State Medical University, Department of Pediatric Stomatology (Dentistry) and Stomatological (Dental) Diseases; Department of Orthopedic stomatology and Maxillo-facial Orthopedics, Iv. Javakhishvili Tbilisi State University, A. N. Natishvili Institute of Morphology2, Georgia.
The goal of the research is to estimate the cell-stromal rate in the gingival subepithelial connective tissue by the histologic and morphometric methods under the modeled gingivitis.
On the basis of morphometric data we can conclude that in experimental condition the change of the relationship between cell and stromal components with a tendency to increase the number of a cell population in the gingival lamina propria is detected, it corresponds to the chronic persistive inflammatory reaction, namely the increase of macrophages and lymphocites number which indicates on the inhibition of the reparative processes.
V. Simonidze, O. Samushia
Structural Changes in Thrombocytes During the Parkinson’s Disease
J. Experimental and Clinical Medicine, 2014, 2, pp. 24-27
Tbilisi State Medical University, Department of Neuromedicine, Akaki Tsereteli State University, Faculty of Medicine, Koutaisi, Georgia
Structural changes in the formed elements of blood during the Parkinson’s disease have been studied. The electronic Tesla (the Czech Republic) microscope - BS -500 and the Opton (Germany) light microscope – “Photomicroscope III” have been applied. The Parkinson’s disease has been diagnosed according to the criteria suggested by A. Hughes and other authors. While studying the structure of thrombocytes, a number of identical structural changes were identified. The study has revealed various shapes of thrombocytes, the production of pseudopodia on their surface, high level of body outline, specific distribution of glycogen granules and their concentration on the periphery, glycogen eruption, dissociation of Alpha-granules towards the edge, and effective outline and density of the granules. There are frequent cases of the granule eruption from the body (exocytosis), the existence of vacuoles on the matrix, the rise in the number of gigantic thrombocytes and, consequently, considerable enhancement of the ability of absorption. Besides, there is a rise in the number of degenerated cells. Mitochondria have round shapes with a dark matrix and rough cristae. The system of open channels and the dense tubular net are clearly visible through the electrogram. The shape of thrombocytes is often changed and stretched on one side. The produced pseudopodia make the impression of participation in phagocytosis. The existing changes prove the activeness of thrombocyte, which must be linked to its involvement in the defensive function against the agents of the body. Thus, the Parkinson’s disease constitutes the diseases of the central nervous system. Besides, changes occur in the formed elements of blood and the processes can be considered to be systemic. Changes in the structure of thrombocytes are expected to be relevant to the changes in brain neurons, since the antigen reaction is conducted through blood in the body. The diseases are believed to develop due to activity of non-specific agents.
V. SIMONIDZE, O. SAMUSHIA
COMPARATIVE ANALYSIS OF STRUCTURAL CHANGES OF WHITE BLOOD ELEMENTS DURING ALZHEIMER DISEASE
J. Experimental and Clinical Medicine, 2014, 2, pp. 27-32
Tbilisi State Medical University, Department of Neuromedicine, Akaki Tsereteli State University, Faculty of Medicine, Koutaisi, Georgia
During Alzheimer disease the studies of white blood elements show more changes in neitrophyles and lymphocytes, while bazophyles and eozinophyles show no significant changes. The research has shown that protective functions are more manifested in neitrophyles, which was manifested by the existence of big pelinuclear area, which shows functional shut off of nuclear and cytoplasm possibilities, which is connected to protective functions. Protective function is manifested also through clamotose, distribution of azurophylic and specific granules in cotoplasm, egzocytosis, osmophilic painting of ribosome, increased number of mitochondria, adhesion of neitrophyles to the thrombocytes and erithrocytes. Therefore, the imagination is created that lymphocytes are in repressed position than the neitrophyles and do not participate in protective reactions. Therefore, during Alzheimer disease the lymphocytes and neitrophyles undergo rough structural changes, lymphocytes are in more depressed position than the neitrophiles and less participate in protective reactions, i.e. the changes refer not only to cellular structures, but also their functions. The disease is affecting not only the central nervous system, but the changes are manifested in functional-structural characteristics of blood form elements. It can be sayd that the disease has systemic character and is developed by the influence of specific agent.
LATSABIDZE L., MACHAVARIANI T., GACHECHILADZE I., GVAMICHAVA T.
J. Experimental and Clinical Medicine, 2014, 2, pp. 34-36
Tbilisi I. Javakhishvili State University, A.Natishvili Institute of Morphology
S. GRDZELIDZE, TS. GAGNIDZE
Study of protective properties of plaferon during chronic Aflatoxicosis
J. Experimental and Clinical Medicine, 2014, 2, pp. 36-39
Ltd “Morphologist”, Kutaisi; TSMU Institute of Medical Biotechnology, Tbilisi, Georgia
Flour mill production environment characterized by high immunosuppressive effects on the workers, which is largely dependent on the intensity of air contamination with mycotoxins. Use of the drug plaferon (famous for its immunocorrective properties), contributed to the normalization of immune homeostasis. Provides advice on applying plaferon at workers hazardous industries, to improve their quality of life.
SH. BOLKVADZE, M.TSIKLAURI
CHARACTERISTICS OF CELLULAR IMMUNITY OF NONUNITED
FRACTURES OF THE TIBIA
J. Experimental and Clinical Medicine, 2014, 2, pp. 39-41
Clinic “Synthesis”, O.Gudushauri National Medical Center, Tbilisi, Georgia
Objective: to study T lymphocyte of nonunions fractures of the tibia.
Material and Methods:We studied 35 patients from 18 to 70 years of contingent clinic "Synthesis " healing with tibial fractures and as a control investigated the characteristics of cellular immunity in 20 patients with a favorable outcome. Studies conducted 9 months after fracture.
Statistical analysis included calculation of means (M), standard deviation, significant differences in performance between groups were calculated by Student's coefficient (results considered significant t> 1,96; p <0,05), analysis was conducted using statistical software package SPSS 17.0
Results: The study found that in patients with Nonunions fractures compared with controls significantly increased CD3 - respectively 69.95+2.201; 74.81+9.878 (p = 0.035) and CD4 respectively 44.98+1.638; 49.36+9.320 (p=0.043). CD8 reveals an upward trend.
T.Gotua, I.Pavlenishvili, C.Parulava, M.Cxaidze, T.Gongadze
Congenital Chronic Diarrhoea caused by Glucose-galactose Malabsorption
J. Experimental and Clinical Medicine, 2014, 2, pp. 41-43
Children’s New Clinic, Tbilisi, Georgia
Glucose-galactose malabsorption (GGM) is a rare autosomal recessive disorder of intestinal transport of glucose and galactose, leading to profuse watery diarrhoea, severe dehydration, matabolic acidosis, failure to thrive, or early death if left untreated. It is caused by mutations in the gene coding for the intestinal brush border of sodium-glucose co-transporter. Treatment includes the elimination of glucose and galactose from diet. We report a female newborn with suspected GGM. She presented with the classical features of Congenital Chronic Diarrhoea .Diagnosis was based on oral glucose tolerance test, stool reducing substences and rule out other diseases with use of laboratory investigations, small-bowel biopsy and histology. Her symptoms rapidly improved with fructose based diet( Galactomin 19, Nutricia)
SH.KHETSURIANI, M.CHITALADZE, K.KHETSURIANI, L.GABUNIA
Enzymes in Staphylococcus aureus strains
J. Experimental and Clinical Medicine, 2014, 2, pp.43-47
Tbilisi State Medical University, Georgia;
St. Andrew the First Called Georgian University of the Patriarchate of Georgia
We studied occurrence of different enzymes (phosphatidylinositol phospholipase C PIPLC, D-xylose dXYL, arginin dihydrolase 1 ADH 1, β-galactosidase BGAL, α-glucosidase AGLU, Ala-Fe-Pro arylamidase APPA, aspirat arylamidase AspA, β-galactopyranosidase BGAR, α-mannosidase AMAN, phosphatase PHOS, leucine arylamidase leuA, propyl amidase-proA, β-glucuronidase BGURr, α-galactosidase AGAl, pyrolidone arylamidase PyrA, β-glucuronidase BGUR, alanine arylamidase AlaA, tyrosine arylamidase TyrA, urease URE, D-maltose Dmal, D-galactosidase DGAL, D-ribose dRiB, lactase-LAC, D-maltase dMAL, D-mannose dMNE, D-raffinosidase dRAF, saccharose SAC, D-tregalose dTRE, argininedihydrolase ADH2s in strains of Staphylococcus aureus. There were determined their putative activity and degree (active, moderately and highly active) of this enzymes. Also the frequencies of manifestation of identification substrates were studied in these strains.
Five most active enzymes – ADH 1, dMAL, dMNE, SAC, dTRE have been revealed in strains of S. aureus; PHOS, PyrA and dGAL by the activity lag behind the above listed enzymes, though URE reveals activity only in one case. 14 enzymes – PIPLC, dXYL, APPA, AspA, BGAR, AMAN, LeuA, ProA, BGURr, AGAL, BGUR, ALaA, TyrA, dRAF were not active, and the rest enzymes – BGAL, AGLU, dRIB, LAC and ADH2S reveal moderate activity. MBdG and OPTO are distinguished by the highest activity. The substrates and indexes – cylcodextrine (CDEX, dSOR), novobiocine (NOVO) and pulurane (PUL) do not reveal activity to any of strains.
G. Abesadze, N. Kiliptari, I. Korinteli, K. Pagava
Modern trends in medical education
J. Experimental and Clinical Medicine, 2014, 2, pp. 47-49
Tbilisi State Medical University, Georgia
In the paper there are discussed some issues of development of medical education. The attention is focused on the ethics, human rights and acquirement of practical skills, the importance and difficulties of teaching bedside skills, the weight of guidelines in the teaching curricula, necessity of feedback from students regarding the teaching process. There is underlined lack of data for drawing the evidence-based recommendations. The research in this direction seems to be very purposeful.
N. LOBZHANIDZE, N. BORCHKHADZE, S. KUPRADZE
Influence of plaferon and zeolite for a Hodgkin's disease
J. Experimental and Clinical Medicine, 2014, 2, pp. 49-52
Kutaisi Regional Blood Bank, Clinic “Hemma”, TSMU Institute of Medical Biotechnology, Georgia
Enabling plaferon-LB and natural Zeolite in chemotherapy for Hodgkin's disease has led to a decrease in the amount of the emergence of resistant forms of the disease by almost 10%. In addition, a comprehensive treatment led to an improvement in quality of life due to the reduced toxicity of the chemotherapy.
M. GUDADZE, K. KANKAVA, A. MARIAMIDZE, G. BURKADZE
DETECTION OF TUMOR PROGNOSTIC MARKERS AND CHARACTERISTICS OF TUMOR STEM CELLS IN INVASIVE DUCTAL CARCINOMA OF BREAST
J. Experimental and Clinical Medicine, 2014, 2, pp. 52-58
Tbilisi State Medical University; National Cancer Centre, Tbilisi, Georgia
Human breast cancer is a truly complex disease with a large inter-tumoral and intra-tumoral heterogeneity resulting in highly variable clinical behavior and response to therapy. The maintenance of the heterogeneity of cells within a tumor is not fully understood. Multiple studies have shown that, CD44+/CD24-low breast cancer tumor cells (breast cancer stem cells belong to this phenotype of cells) are characterized with malignant features. Purpose of our study was Identification of possibility of interaction between Prognostic factors of breast cancer (the size of the primary tumor, state of Lymph nodes, status of ER/PR receptors, characteristics of HER2 expression) and CD24, CD44 positive cells. For research was used 1324 postoperative breast cancer samples, from which were selected 393 patient with invasive ductal carcinoma samples. The age range was between 23-73 years. For all cases was performed immunohistochemical study using ER, PR, Her2, Ki67, CK5- molecular markers. Obtained quantitative statistical analyses were performed by using SPSS V.19.0 program. Statistically significant were Considered 95 % of confidence interval. Amount of cases of stem cells with CD44+/CD24-low phenotype increases in direct proportion with to the size of the tumor and achieves peak in T4 tumors. Amount of such cases is also high in LN positive cases and in cases with high index of Ki67.
M.IZRAELASHVILI, Z.CHOMAKHASHVILI, N.ZOSIDZE
SURGICAL ANATOMY OF LIVER CAVAL PORTA
J. Experimental and Clinical Medicine, 2014, 2, pp. 58-61
Batumi Shota Rustaveli State University, Georgia
Resulted research in has been established that the right and left hepatic veins were found in all cases, but the middle hepatic vein was other found in 3.4% cases. Among the other hepatic veins we often could not find the right (57.4%) and left (63.4%) dorsal veins and left fissural vein (37.9%). While draining of the right and left portal lobes of liver some individual features were found. One of the key moments of anatomic resection is crisscross of hepatic veins in the caval porta, nearby their joint with postcava. In relation with the above, great practical importance is attached to relations of terminal parts of hepatic veins. In the process of research it has been established that resulted detailing we can distinguish four types of junction of research it has been established that resulted detaling we can distinguish four types of junction of the left and rights veins. Conditions of approach to the terminal parts of postcava are also determined with the shape of caval porta of liver, which may be open, close or transitional. It has been established that condition of crisscross of hepatic veins at the place of their joining with postcava are individual and depend on length of terminal parts of hepatic veins, their size and angle of their joining with postcava. Resulted research we conclude that the conditions of veins crisscross in conditions of open porta are more admissible for surgeon, than in condition of close porta.
G.Megrelishvili, L. Dzneladze, N. Khmaladze, S. Lominadze
Reasons and features of mesenteric circulation acute disorders
J. Experimental and Clinical Medicine, 2014, 2, pp. 61-67
Tbilisi 5th Clinical Hospital, Georgia
The article discusses 146 cases of acute disorders of mesenterial blood supply (ADMBS). The medium age of the patients was 68,4±3,6 years. The oclusive form made up 88% of the mentioned disease. (98.1% of them were due to damage of the arterial system of the gut). The main reason of pathologies ADMBS in 96% of the patients has been atherosclerosis of aorta and/or of its odd visceral branches. The reasons of severe mesenterial ischemia in 87.6% of the cases were occlusion of mesenterial vessels, what was due to trombosis and ambolia of odd visceral branches of abdominal aorta. The trombosis of portomesenterial part was evidented rearly in 2.1 % of the cases.
TS.PARULAVA, M.CHKHAIDZE, T.GOTUA, M.TSANAVA, I.KHURTSILAVA
CLINICAL FEATURES, DIAGNOSIS AND MANAGEMENT OF CROHN, S DISEASE
IN CHILDREN AND ADOLESCENTS
J. Experimental and Clinical Medicine, 2014, 2, pp.67-73
Children,s New Clinic, Tbilisi, Georgia
Inflammatory bowel disease (IBD) is comprised of two major disorders: ulcerative colitis (UC) and Crohn's disease (CD). Approximately 25 to 30 percent of patients with CD and 20 percent of patients with UC present before the age of 20 years. Adults and children with IBD may present with similar clinical features, however, children can develop unique complications, including growth failure and delayed puberty. Accurate management would have potential benefits with assessing disease prognosis. The present review highlights the key issues that might be used in clinical practice.
Leptospirosis in Adjara
J. Experimental and Clinical Medicine, 2014, 2, pp. 73-76
Shota Rustaveli State University, Batumi, Georgia
Results of a poll of dentists Adjara
J. Experimental and Clinical Medicine, 2014, 2, pp. 76-84
Shota Rustaveli State University, Batumi, Georgia
Special working features of stomatology distinguish it from other medical sciences. That makes necessary establish ergonomic environment of work dentists and follow all necessary principles. Stomatological problems also include adequacy equivalence of doctors working environment and its matching with ergonomic principles. This topic remains a problem, because great numbers of dental offices are arranged by paying much attention only to cosmetic features and interior arrangement and very little time is spent on ergonomic questions.
The paper presents the major indices or ergonomic expertise in stomatology institutions of Adzhara, Georgia. It was shown that 43% of doctors-stomatologists express their displeasure for their working conditions. Almost half of the doctors suffer from various diseases which are caused specificity of labor of stomatologist and by unsatisfactory labor conditions. In these organizations the major principles of the President (No 564 of 01.10.1999) and the Minister of Health of Georgia (No309\N of 05.11.2002) are violated, which affects the quality of the jobs performed by the doctors and thus in fringe the rights of patients.
N. KORSANTIA, A. KATSITADZE
PLAFERON ADHESIVE FILMS AND CLINICAL-IMMUNOLOGICAL ASPECTS OF TREATMENT OF OPHTALMIC HERPES
J. Experimental and Clinical Medicine, 2014, 2, pp. 84-87
Department of Dermatology and Venereology of TSMU, Tbilisi, Georgia
It was shown that the scheme of treatment of herpes infection of eyes (acyclovir and a local immunotherapy – plaferon adhesive films on a conjunctiva) was effective in 91,5% of cases, in comparison with monotherapy by an acyclovir – 71,1%. It is clinically noted – reduction of terms of a resorbtion of herpetic elements and aggravation knocking over, reliable increase in duration of remission. Indicators of clinical symptoms were in direct correlation with a condition of immunocompetence of an organism.
N.V. Tsiskarishvili, A.G. Katsitadze, Ts.I. Tsiskarishvili
Xerosis problem and its solution, with taking into account the time based organization of physiological processes
J. Experimental and Clinical Medicine, 2014, 2, pp. 87-90
TSMU Department of Dermatovenerology, Tbilisi, Georgia
Dryness of skin or Xerosis (from the Greek, "Xeros"- dry) - a common problem encountered in everyday practice of dermatologist and cosmetologist. Dry skin is characterized by reduced elasticity, easy peeling and easily occuring irritation, subjectively pruritus and paresthesia are noted. Xerosis might be considered as a symptom of pathological process as well as the result of skin involution in practically healthy people of older age. During the treatment of this pathology, along with the specific treatment of the underlying disease it is essential to apply external therapy, including emollient means.
As we know the level of secretion and composition of sebum is influenced by circadian rhythm with rise in the morning and decrease at night. Control of the time factor for application of external therapy, including moisturizing and emollient creams and knowledge of the organization of physiological processes in patients organism allow to improve the quality and effectiveness of treatment and prevent relapses. Based on the aformentioned the aim of the study was to develop a comprehensive treatment of patients with xerosis taking into account circadian variations of the skin barrier functions.
The results showed that the use of BIA in skin xerosis patients revealed significant deviations from the norm, and in patients with senile xerosis changing of these parameters coincided with the circadian rhythm changes in morphological and functional features of the skin, while in patients with dry skin, biorhythm of these changes was disrupted as a manifestation of the pathological process. Applying the cream "DEXERYL" (developed by dermatological laboratory "Pierre Fabre" France), in the complex treatment of patients with xerosis (taking into account the daily changes in parameters, reflecting the state of the skin barrier) had a pronounced therapeutic effect.
T. Gvamichava, I. Gachechiladze, T. Machavariani
VASCULARISATION OF COLON AND BREAST CANCER
J. Experimental and Clinical Medicine, 2014, 2, pp. 90-94
I. Javakhishvili Tbilisi State University, A. Natishvili Institute of Morphology, Tbilisi, Georgia
Vascularisation of the colon and breast tumors by histologic, electron microscopy and electron radioautographic methods were studied.
The activation of angiogenesis occurs mainly in the areas of active tumor growth. The intensive proliferation of endothelial cells, which is detected by 3H-thymidine were revealed. The stage of comperative differentiation mainly occurs in the capillary endothelium. In such capillaries endothelial cells revealed by 3H-uridine. The regression stage of single microvessel accompaning by changes in endothelial cells (nuclei pyknosis, cytoplasm flattening and homogenisation, organelles disorganisation and etc.). These steps developed asynchronously.
N.G. Khodeli, Z.A. Chkhaidze, O.D. Pilishvili, A.V. Dgebuadze
Development of Atraumatic Methods of Moving Blood in Artificial Circulation Systems
J. Experimental and Clinical Medicine, 2014, 2, pp. 94-96
Institute of Morphology of Tbilisi State University, Georgia
The traumatic impact of roller pumps on blood cells and homeostasis in general is well known. To reduce blood cell trauma, we have developed a heart-lung bypass machine (HLBM), in which the movement of blood occurs by a new method. The method is based on the principle of moving blood volume by pressure and vacuum consecutively in sealed reservoirs at placed on different levels. Evaluation of the methods was performed on stand-testing and in 17 experiments on animals. Effectiveness of the system was determined: blood flow rate (up to 4 l/min); height of displacement (1.5 m); vacuum level (up to -10 mm Hg). Tests have shown the reliability of the whole system, the absence of hemolysis during 4 hours operating the HLBM.
N.G.Khodeli, Z.A.Chkhaidze, D.D.Partsakhashvili, O.D.Pilishvili,
NONTRADITIONAL APPROACH TO CREATING A SYSTEM FOR CARDIOPULMONARY BYPASS IN HEART SURGERY
J. Experimental and Clinical Medicine, 2014, 2, pp. 96-98
Department of Clinical Anatomy and Operative Surgery of Faculty of Medicine of TSU
We are looking at the possibility of creating a device for heart-lung bypass without using an oxygenator and roller pumps. The foundation of this method is the principle of biventricular bypass that does not require oxygenator usage. Instead of the pumps, the mechanism uses hermetic cardiotomic blood reservoirs. The injection is carried out by creating positive pressure in the reservoir chamber. From reservoir the blood flowed into a canula of either the lung artery or the aorta, performing central and peripheral blood circulation. The system was tested in vitro, and revealed a stroke capacity of up to 7,0 l/min. On animal experiments, the system functioned successfully for up to 5 hours.
M. Okujava, G. Sulaberidze, K. Liluashvili, S. Bezarashvili
Importance of Dietary Fiber in Management of Risk-factors for Cardiovascular Diseases
J. Experimental and Clinical Medicine, 2014, 2, pp. 98-100
Tbilisi State Medical University, Georgia
High levels of dietary fiber intake have beneficiary effect and are associated with significantly lower prevalence of major risk-factors of cardiovascular diseases (CVD), namely hypertension, diabetes, obesity and dyslipidemia. Soluble dietary fibers decrease serum cholesterol, most effectively LDL cholesterol concentration. Dietary fiber reach food intake is associated with reduced blood glucose and HbA1c levels, greater satiation and satiety and decreased body weight. Dietary fiber intakes around the world are less than half of recommended amount, therefor increasing dietary fiber consumption for CVD prevention is a critical public health goal.
L. Adeishvili-Andguladze, M. jokhadze, R. Makharadze,
T. kirvalidze, m. gonaShvili
The Development of Modified Thin Layer Chromatography Method (TOXI-LAB) for Determination of Desomorphine in Biological Feuids
J. Experimental and Clinical Medicine, 2014, 2, pp. 100-103
TSMU, Department of Pharmaceutical and Toxicologycal Chemistry; TSMU, Department of Pharmacognosy and Botanics; Tbilisi, Georgia
The modified method of thin layer chromatography, so called TOXI-LAB is on the modern screening method, which is widly used in analysis of narcotic and psychotropic agents. Therefore the utilization of this technologic is very actual.
The aim of this study was the development of the method of determination of the Desomorphine in biological fluids – urine, blood, plasma.
The study method was modified thin layer chromatography TOXI-LAB. Research was performed under the demands of the company “TOXI-LAB A” proceed system, by utilization of same equipment and indexes. As a solvent for mobile phase was selected ethilacetate-methanol-deionised water and Dragendorf reagent (modified).
As a result of conducted studies was clearly appeared, that presented method provides the high resolution of identification an separation of desomorphine from biological fluids.
L. Adeishvili, M. jokhadze, R. Makharadze,
T. kirvalidze, m. gonashvili
Heterogenic Immunoassay screening method (ELISA)
development for determination of Desomorphine
J. Experimental and Clinical Medicine, 2014, 2, pp. 103-105
TSMU, Department of Pharmaceutical and Toxicological Chemistry;
TSMU, Department of Pharmacognosy and Botanicks
Immunoassay method is widly used in chemical-toxicological analysis of narcotics. There is two types of Immunoassay method: homogen and heterogen.
The aim of the study was development of Desomorphine determination in biological fluids.
The subject of the study – the urine, blood and plasma of live and death persons. Methods of analysis heterogen Immunoassay analysis (ELISA).
Were developed the heterogen immunoassay method of (ELISA) for determination Desomorphine in biological fluids.