2014, ¹ 2


1. Nachkebia R., Isakadze A.


J. Experimental and Clinical Medicine, 2014, 2, pp.11-13


Pharmaceutical company “Salkhino”, Tbilisi, Georgia


Georgia is famous for its rich, ecologically clean flora. The method of preparing the medicines worked out by our ancestors is justified due to authentic healing properties. The medicines “Salkhino”, “Pherdy”, “Antioxidant” made by pharmaceutical company “Salkhino” include large amount of medical substances, which have soothing, painkilling, antimicrobial, antioxidant and spasmolitic properties. They have high immunomodulating effect. Antioxidant activity in the drug “Salkhino” is 39-52%, in “Pherdy” – 72-85%, “Antioxidant” – 55-68%, respectively.

The positive therapeutic effect of the noted medicines proved by cured patients, documental material, audio-video records and clinical-laboratory researches carried out by medical apparatus.




Prognostic value of heart rate changes during mild mental

stress and exercise stress test in the prediction of sudden cardiac death in Indian healthy population


J. Experimental and Clinical Medicine, 2014, 2, pp.14-18


Yerevan State Medical University after Mkhitar Heratsi, Armenia


The aim of our study was to evaluate prognostic value of heart rate (HR) changes during mild mental stress (MMS) and exercise stress test (EST) in the prediction of sudden cardiac death (SCD) in general population.

Large HR increase during EST was associated with a significantly lower risk for SCD and the relative risk (RR) of the third vs. the first tertile was 0.49 after adjustment for the confounders. The risk of SCD increased progressively with an increase in HR during MS and the RR of the third vs. the first tertile was 2.37 after adjustment for confounders. The correlation between the two HR measures was very week (0.19). The highest proportion of SCD was found among the subjects who increased their HR the most during mild MS and the least EST. After adjustments for the confounders, subjects with an elevated HR increase during mild MS and a mild increase during EST had the highest RR of SCD, while subjects with a small HR increase during MMS and a large HR increase during EST had the lowest RR. 

Our results show that the HR increase during a MMS in preparation for EST is a strong predictor of SCD. Specially, individuals with the largest HR increase during MMS and the mild increase in HR during EST are at higher risk.



3. Simonia G.,  Andronikashvili I., Pantsulaia I., Basishvili N., Gujabidze L.

The Role of Endogenous Ouabain andMarinobufageninin the Development of Salt-sensitivity and Salt-sensitive Hypertension

J. Experimental and Clinical Medicine, 2013, 4, pp.19-22


Department of  Internal Medicine of Tbilisi State Medical University, Hypertension Research Center, Tbilisi, Georgia.


Among  humoral factors determining salt-sensitivity so called endogenous cardiotonic steroids that inhibit Na,K-ATPase – endogenous ouabain (EO) and marinobufagenin (MBG) have been considered. The goal of our study was to elucidate the role of EO and MBG in the development of salt-sensitivity and salt-sensitive hypertension in Georgian patients.The objectives of the study were: Typing of normotensive and hypertensive subjects by salt-sensitivity test, and detection of circulating levels of EO and MBG in salt-resistant and salt-sensitive normotensive subjects during low and high salt diet.; Our results have shownthatdevelopment of salt-sensitivity and salt-sensitive hypertension might be due to increased production of marinobufagenin and endogenous digoxin – so called cardiotonic steroids and provide an useful tool for identifying salt-sensitive risk groups among healthy population and prevent formation of essential hypertension.


4. Morchadze L., Margvelashvili V.

The optimization of dental activities for patients with high emotional tension on the example of the population of Imereti region, Georgia

J. Experimental and Clinical Medicine, 2014, 2, pp.23-30


Kutaisi Regional Stomatological Center, Tbilisi Dental Clinic ,,Royal Dent”, Georgia


It’s for the first time that investigation of negative stomatological experience was undertaken among the population of Georgia. What was revealed is its high spreading, reasons, peculiarities depending on age, gender, state of mouth cavity. It is shown that extreme attention must be played to senior patients, having definite stomatological manipulations’ fear. A rationalization proposal is made on interpreting the total value of emotional burning syndrome (a king of professional deformation of a person having to communicate with people at work), which enables to efficiently evaluate the degree of the syndrome manifestation and its impact on psycho-emotional state of a person.

Thus it is proved that stomatological aid to the population grew more effective medically, with the use of psychotherapeutic correction of emotional tension during dental treatment. The effect reaches 40% for planned patients, 60% for the patients suffering acute toothache syndrome. The data were received by measuring of the reduction of skin-galvanic reaction value. In connection with that, we consider it necessary to recommend the method of emotional tension correction for patients getting dental treatment, to be used in health care practice, because of its high efficiency, to improve safety and quality of the dental treatment of population.


5. Nikolaishvili M., Jikia G., Bagdavadze M., Turashvili E., Tavadze N.,

Dumbadze S., Gegenava N., Kopaleishvili U., Mandaria Q., Nergadze Q.,

Gogiashvili Z., Kakashvili O.

Influence of eco-toxicological stress factors on the bank strip

of river Mtkvari

J. Experimental and Clinical Medicine, 2014, 2, pp.30-34


I.Beritashvili Centre of Biomedicine, Tbilisi, Georgia


Based on conducted observations it had to be sad that concentrations of these basic cations (K+, Na+, Ca2+)  along river Mtkvari current increases and does not exceed allowed limit concentration. But SO42- ions increases that indicates on strengthening of antropogenic load in surface waters. On fecal contamination and strengthening of eutrophication processes indicates contents of biogenic elements (NO2-, NO3-) that increase along river current and reach maximum in town Rustavi and Vere river canyon where their concentration (0,02 mg/l) several times exceeds maximum allowed concentration.



6. Shonvadze D., Korinteli I.A., Sharikadze P., berikashvili D.,

Kiseliova T., Pagava K.

To the question of diagnostics of diseases with autoinflammatory periodic fever in children and adolescents

J. Experimental and Clinical Medicine, 2014, 2, pp.35-37


Tbilisi State Medical University, Georgia


In the paper there are presented the elements of expert program aimed to support diagnostics of the diseases with autoinflammatory periodic fever in children and adolescents. The results of its clinical validation are presented.




J. Experimental and Clinical Medicine, 2014, 2, pp.37-40


The clinic “Traumatologist”, David Agmashenebeli Tbilisi State University, Georgia


To conduct a comparative analysis of the results of treatment with transcervical fractures of the femur bone using a three-bladed nail and a V-shaped fixation.

The study is based on a comparative analysis of treatment of 150 patients with femoral bone fractures, in age from 20 to 80 years. Of these 100 patients was conducted osteosynthesis V-shaped fixation and 120 patients with three-bladed nail.

Statistical processing was performed using SPSS 17.0 statistical software differences compared values ​​were considered significant at p<0.05 using the criterion χ2-square. Assessment of risk of complications was performed according to the methodology of evidence based medicine.

Comparative analysis of the results of treatment with transcervical fractures of the femur bone confirms the effectiveness of treatment by a V-shaped fixator as compared to using a three-bladed nail. In the treatment of three-bladed nail significantly increased risk factors delayed consolidation – RR = 1.37 (95% CI = 1.06-1.77); failure of union fracture – RR = 1.50 (95% CI = 1.07-2.10); avascular necrosis of the femoral head – RR = 1.49 (95% CI = 1.16-1.92).



8. Kraveishvili S., Shonia N.,  Sakvarelidze Z., Sakvarelidze N.

Prevalence and Intensity of Dentition Defects and Secondary Deformations in the Population of 25-35 Age Group


J. Experimental and Clinical Medicine, 2014, 2, pp.41-45


Department of Orthopedic Stomatology and Maxillofacial Orthopedy, Tbilisi, Georgia


Partial secondary adentia together with caries and parodont diseases, is among the most widespread diseases of dentofacial system, correlated, in addition, to the number of lost teeth, with the age.  Improvement of the methods of prevention and treatment of the above diseases is one of the most significant problems in the dentistry. Effectiveness of dental assistance organization and planning is based on the epidemiological studies. In Georgia, epidemiological studies were conducted in the populations of various age groups in previous years though, according to our data, no studies of dentition defects and deformations were conducted in the recent decades. Goal of our research was study of prevalence and severity of dentition defects and deformations, regarding sizes of existing defects, their localization, causes and types of deformation in different age groups, for development of specialized dentistry assistance plans and prevention programs. We have studied   31 patients  of ages between 25 and 35 years. We have developed special questionnaire. Researches showed that in the studied age group (25-35), 96,8% (30 patients),  had secondary adentia. Most of them had the defects in the buccal teeth area. All 31 patients required orthopedic treatment but only 6 (19,35%) of them have visited the clinic for this purpose while 25 (80,65%) of them were unaware about need of prothetic assistance. Regarding significance of the mentioned problem, the obtained results show the need of timely orthopedic intervention for the purpose of prevention of further complications. For this, specialized dental assistance plan and prevention measures’ program should be developed.




J. Experimental and Clinical Medicine, 2014, 2, pp.45-50


Tbilisi State Medical University, Georgia


Anti-tumor preventive efficacy of phage lysates of E.coli and Ps.aeruginosa has been studied in 2-3 months non-purebred male mice bearing transplanted Ehrlich carcinoma. Regimen of vaccinations was: 1) single – 0,25 ml phage lysates intraperitoneal injection 3 days before Ehrlich carcinoma inoculation (1x106 tumor cells);  2) 3 times vaccinations – 3, 6, and 9 days before inoculation of carcinoma; 3) 10 times vaccinations (during 10 days), before inoculation of carcinoma. Treatment efficacy was evaluated according to the indices of cancer growth (development of cancer tissue, cancer growth inhibition percent, lifespan and survival percent).  Experiments have shown that single and 3 times preventive vaccinations inhibited tumor development and delayed malignant growth, while, 10 times permanent vaccinations had no effects on cancer growth. Cancer growth inhibition percent in single and 3 times vaccinated animals with E. coli phage lysate were 58% and 58.1%, and in case of Ps. aeruginosa –  50% and  56% on the average. Maximal lifespan of mice in control groups were  57 days  on the average,  while, by the 125th day of cancer growth, at single vaccination, the 17% of mice were alive, and in 3 times vaccinated mice the survival percent was 25%. At vaccinations with Ps. aeruginosa phage lysate it was 7% and 20% respectively. Anti-tumor potential of bacterial page lysates supposedly could be explained by immunoregulatory properties of the preparation stimulating innate immune responses of the organism.




J. Experimental and Clinical Medicine, 2014, 2, pp.50-55


Tbilisi State Medical University,

Dental Clinic, Training and Research Centre “UNIDENT”, Tbilisi, Georgia


The replacement of a single anterior tooth, utilizing a treatment modality that achieves optimal aesthetic and functional results while leaving the adjacent dentition intact, is one of the most difficult challenges for the restorative dentist. Of the options available for this type of restoration, each has its own advantages and disadvantages and some of the earlier treatments have lost their acceptance due to patient demand for conservation of the adjacent dentition. For example, a three-unit bridge, which conventionally reduces the adjacent teeth, is no longer a primary alternative in today’s dentistry of minimal invasion. The etch-bonded Maryland Bridge gained acceptance as a conservative aesthetic restoration in the replacement of the missing anterior (or canine, premolars) dentition.

The learning objective of this article is to review the methods currently utilized and to present a recently introduced treatment modality – the three component composite bridge.




11. Nikolaishvili M., Beriashvili S., Franchuk K., Tupinashvili T.,

Vashakidze I., ZENAISHVILI S.

Thyroid dysfunction and oral mucosa mineralization in teenagers

J. Experimental and Clinical Medicine, 2014, 2, pp.56-59


D.Agrmashenebeli State University; I.Beritashvili Centre of Biomedicine, Tbilisi, Georgia


A new absorption line on the oral mucosa spectra is not observed in teenagers with thyroid dysfunction, but there are some significant qualitative and quantitative changes noted, indicating to decrease in the mineral component concentration of the oral mucosa.  Saliva can be considered as a biological fluid, exceptional qualities of which contribute to maintaining of the mineral components of homeostasis in the oral cavity. Literature data and our clinical study results allow us to conclude that in planning stomatological, treating and preventive measures the characteristic changes in teenagers’ thyroid dysfunction must be taken into consideration.



12. murvanidze e.

Effectiveness of goal-oriented adaptive physical exercises in children with disabilities

J. Experimental and Clinical Medicine, 2014, 2, pp.59-62


Center of Children Neurodevelopment, Tbilisi, Georgia


13. Tsagareishvili N., Bakuridze A., Kurdiani N., Zardiashvili L., Jikia T.

Determination of some pharmacokinetic parameters of the tablets "Genthin"

J. Experimental and Clinical Medicine, 2014, 2, pp.62-65


Tbilisi State Medical University, Department  of Pharmaceutical Technology, Georgia


The purpose of the investigation consisted in determination of distribution dynamic of main active ingredient of the tablets “Genthin” – mangiferin in biological material (liver, kidneys, heart, lungs, blood;) in vivo experiments (rabbits).

High Performance Liquid Chromatography technique served as assay method for quantification of mangiferin  in biological material and distribution dynamic of this biologically active ingredient  was determined after oral administration of the tablets. It was established that in liver and kidneys the maximum concentration is reached in 4 hours and corresponds accordingly to 58 ng/g and 38 ng/g , while in the heart and  lungs the maximum concentration is reached in 6 hours and corresponds accordingly to  24 ng/g and 21 ng/g;

During study mangiferin plasma concentration-time profile was determined and some pharmacokinetic parameters established:  elimination rate constant  - 0,1875 1/h, absorption rate constant  -  0,597 1/h, elimination half-life - 3,696 hours, absorption half-life -  1.16 h, the maximum concentration -  42 ng/ml,  time to reach peak concentration -  4 hours.






Aging and immune system

J. Experimental and Clinical Medicine, 2014, 2, pp.66-79


Tbilisi State Medical University, Agricultural University of Georgia


Natural  process of ageing  is considerably determined by deterioration the of immune system, called immune senescence, which in turn is  affected by genetic and environmental factors and lifestyle. Age related modulations of immune system leads in many cases to the development of immunodeficiences and chronic inflammations, which accounts for vaccine inefficiency, development of autoimmune disorders, oncologic and infection pathologies.    

Although certain types of immune cells (NK) could increase in number, overall functional  activity of all types of immune cells  irreversibly decreases with  age. Thymus involution  is one of the main changes that develops in immune system with age, which consequently deepens  the deterioration of all immune processes.  In  particular,  naïve-T  compartment  decreases,  while  memory  T  cell  compartment  gets enhanced.  Besides, considerable  limitation  of  immune responses  are  mainly  caused by restriction  of lymphocyte repertoire (oligoclonality) and appearance of terminal differentiated anergic cells. The extent of  immune  system  deterioration  depends  whether  aging  process  could  be  defined  as  premature, phisiologic or successful.   

  Discovery of immunobiological  markers  of  longevity holds  great clinical importance.  Study of mechanisms of immune senescence and the factors that influence it are immensely valuable in unraveling the secrets of successful aging.



15. Kintraia N., Melia L., Sulukhia R.

Simultaneous  operations in  obstetrical practice:


J. Experimental and Clinical Medicine, 2014, 2, pp.80-82


O.Gudushauri National Medical Centre; Chachava Scientific- Research Institute of Perinatal Medicine and Obstetrical-Gynaecology, Tbilisi, Georgia


Our studied is admitted to confirm the advantage of simultaneous operation during  obstetrical and surgical pathologies. We studied 43 simultaneous operations in obstetrical which included caesarean section and herniaplastic.

The age of patients ranged from 21 to 36. It was 35 routine planned operation, 7 was urgent. None of the operations were followed by complications due to the increased scop of  volume and dimensions of surgical procedures.