J. EXPERIMENTAL & CLINICAL MEDICINE
2016, ¹ 5
1. S. KUPRADZE, B. KORSANTIA, T. TSINTSADZE, I. GVELESIANI, L. KAJAYA 9--19
WHY MEDICINE CAN NOT COPE WITH FLU
Tbilisi State Medical University, V. Bakhutashvili Institute of Medical Biotechnology,
J. Experimental and Clinical Medicine, 2016, 4, pp.9-19
The article presents an analysis of published data on the structure of the influenza virus and its reproduction in sensitive cells, genetic and immunogenic variability, characteristics of the epidemiology of influenza infection. Particular attention is paid to the prevention of influenza, methods of creating different types of influenza vaccines.
The authors examine the mechanisms of resistance of viruses and relatively low efficiency of vaccines during epidemics of influenza. In particular, it points to the difficulty of obtaining highly specific vaccines and the impossibility of their timely preparation for the expected epidemic. In order to solve this problem, offered an interesting biotechnological ways to accelerate the availability of influenza vaccine.
2. Î.Î. SYZON
SPECIAL FEATURES OF CLINICAL CLASSIFICATIÎN ÎF PSÎRIATIC DISEASE
Danylo Halytsky National Medical University of Lviv, Department of Dermatology and Venereology, Ukraine
J. Experimental and Clinical Medicine, 2016, 4, pp.20-30
The paper develops clinical and diagnostic criteria to determine psoriatic disease phenotype by analyzing national and foreign literature, personal experience. The specified criteria are required in the work of dermatologists as supportive and auxiliary ones when an extensive diagnosis of psoriatic disease is established, in differential diagnostics and assessment of clinical and therapeutic effect of offered treatment. In order to improve classification criteria and to put them closer to 100% by their specificity and sensitivity the authors present a questionnaire for standardized testing and examination of psoriatic patients.
3. N. KIKODZE, I. PANTSULAIA, T. CHIKOVANI
NANOPARTICLES IN BIOLOGY AND MEDICINE
V. Bakhutashvili Institute of Medical Biotechnology, Tbilisi State medical University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.31-35
Nanoparticles can be engineered with distinctive compositions, sizes, shapes, and surface chemistries to enable novel techniques in a wide range of biological applications.
This review will provide an overview of various types of nanoparticles and concepts of targeting nanoparticles. We will also discuss the advantages and recent applications of using nanoparticles as tools for drug delivery, imaging, sensing, and for the understanding of basic biological processes.
4. M. GOGOLADZE, M. KILADZE, T. CHKHIKVADZE, F. KHUTSISHVILI, D. JIKIA
BACTERIOLOGICAL DESCRIPTION OF “NEW” ASEPTIC MESHES
J. Experimental and Clinical Medicine, 2016, 4, pp. 36-40
Preventing postoperative infectious complications is still one of the unsolvable issues of the surgery. Mesh associated postoperative, infectious comprise 3-5%. The same figure equals to 1-8% in case of planned surgeries. The purpose of our study is improving treatment results of hernias and preventing infectious complications.
Experiment included 21 rabbits, they were sewed ‘’GRENA Ltd‘’(manufacturer company) light-weight meshes on two different kinds (treated differently). Each rabbit had 6 meshes, there were 126 meshes total. After removing meshes trom animals they were bacteriologically studied with preliminary planned schedule. Mesh contamination with S. epidermidis, C. albicans and E. coli strains were noted.
Conducted microbiologic study estimated and confirmed “Koladerm” + chlorhexidine treated mesh special antimicrobial features, which ensures infection suppression in optimal time and more reliability of herniology.
5. Ts. ZHORZHOLIANI, L. ZHORZHOLIANI
CLINICAL-PSYCHOLOGICAL ASSESSMENT OF SCHOOL ADAPTATION OF
PRIMARY-SCHOOL AGE CHILDREN
D. Aghmashenebeli Georgian University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.41-47
Goal of the study was clinical-psychological assessment of adaptation ability of the primary-school age children residing in Georgia and phenomenological analysis of deadaptation disorders.
One-phase study was conducted on the basis of 4 public schools of Batumi. 450 students aged from 6 to 9 were studied. As a result of conducted clinical-psychological study, school adaptation disorders were identified in 20-40% of children. Deadapation syndromes were presented at both, personal level (depression, anxiety, despondency, restlessness, neurotic symptoms) and in relations with the surrounding people (distrust, enmity to the strangers and peers).
Study findings allowed identification of the children group requiring individual medical-psychological correction in a timely manner.
6. M. JAPARIDZE, A. GVENETADZE
COMPARATIVE ANALYSIS OF TOTAL LAPAROSCOPIC (TLH) AND VAGINAL HYSTERECTOMIES (VH) – POSTOPERATIVE QUALITY OF LIFE
“Aversi Clinic”, “Z. Sabakhtarashvili Clinic”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.47-50
Purpose: To compare postoperative outcomes e.g. quality of life after total laparoscopic hysterectomy (TLH) and vaginal hysterectomy (VH).
Methods: 171 women gone through hysterectomy were questioned in 8 weeks after the operation: TLH (n = 81); VH (n = 90). The questionnaire was as follows: 1). Do you have at the moment postoperative complains? 2). Did urination change after operation? 3). Did defecation change after operation?
Results: From questioned 171 patients 4 had postoperative complains (2,3%): 2 in laparoscopic hysterectomy – 2,4%, 2 in vaginal hysterectomy group – 2,2%.
On the 2nd question 151 patients answered: a). did not change (88,3%) – 71 from TLH group (87,6%), 80 from VH group (88,8%); b). improved – 18 patients (10,5%) – 8 from TLH group (9,8%), 10 from VH group (11,1%); c). worsened – 2 patients (1,1%),1 from TLH group (1,2%), 1(1,1%);
On the 3rd question – did defecation change after operation, 158 patients answered: a). did not change (92,4%) – 73 from TLH group (90,1%), 85 from VH group (94,4%); 4 patients (2,3%) answered b). improved – 8 from TLH group (2,4%), 2 from VH group (22,2%); 9 patients (5,2%) answered c). worsened – 4 from TLH group (4,9%), 5 from VH group (5,5%);
Conclusion: As a result, we conclude that the quality of life in two months after laparoscopic and vaginal hysterectomy does not worsen and is practically similar in these two methods. More studies are needed to evaluate postoperative quality of life in long-term period and by other criteria, as libido and sexual function.
7. L. EPRIKASHVILI, T. KORDZAKHIA, M. ZAUTASHVILI, G. TSINTSKALADZE,
N. PIRTSKHALAVA, M. DZAGANIA, N. BURKIASHVILI
ANTIBIOTICS IN THE ENVIRONMENT
Iv. Javakhishvili Tbilisi State University; P. Melikishvili Institute of Physical
and Organic Ñhemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp. 50-55
We consider some of the environmental problems associated with the pollution of water objects, which are an essential component of nature. The consequences of water pollution by various pollutants, in particular antibiotics, pose the greatest threat to the environment and, above all, to human health and all living organisms. Use of natural zeolites as granular filter material in the process of a special deep-water treatment in order to improve its health indicators prior to use for drinking and some industrial purposes is one of the promising areas for water conservation.
8. G. GURGENIDZE, D. LABARTKAVA, N. URUSHADZE
AN ASSESSMENT ALGORITHM FOR LABORATORY (IMMUNOLOGIC) EVALUATION OF THE STEROID-SENSITIVITY IN PATIENTS WITH BRONCHIAL ASTHMA
V. Bakhutashvili Institute of Medical Biotechnology of Tbilisi State Medical University,
J. Experimental and Clinical Medicine, 2016, 4, pp.55-59
On the basis of clinical assessment and laboratory (immunology) testing of 72 asthmatic patients and 34 healthy volunteers, with further evaluation of correlation and statistical significance of above-mentioned data, it have been elaborated the strictly objective immunologic (laboratory) criteria (the diagnostic algorithm) for determination of steroid-sensitivity in this population. These laboratory criteria, including testing of in vitro steroid-seneitivity of peripherial blood lymphocytes and basophils, as well as determination of serum anti-lipocortin autoantibody titres, will be helpful for prediction of the responsiveness of each individual patient to the planned steroid treatment with respective evaluation of their possible risks and expected benefits.
9. T.SHULAIA, N.KILADZE, N.KORSANTIA
BAZEX SYNDROME IN THE PATIENT WITH GASTRIC ADENOCARCINOMA
Department of dermato-venerology, TSMU; Medical Center “Marjani”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp. 59-62
In the clinical picture of many malignant diseases often are developed skin syndromes, which are not directly related to the localization of the tumor itself or its metastases. Paraneoplastic syndromes represent a heterogeneous group of dermatoses, the early development of which contributes to the early diagnosis of tumor process and, therefore, earlier and more effective treatment. The present study describes the case of obligate paraneoplastic syndrome – Bazex syndrome (paraneoplastic acrokeratosis) associated with adenocarcinoma of the stomach. The appearance of this syndrome in this gave a possibility to confirm the presence of neoplastic process at the earliest stage and provide effective treatment.
10. I. VARDZUKASHVILI, SH. SAXVADZE, I. TABORIDZE, L. ALADASHVILI, M.VARDZUKASHVILI
CORRELATION OF PHYSICAL DEVELOPMENT PARAMETERS WITH THE RESULTS OF SURGICAL TREATMENT SCOLIOSIS IN PATIENTS WITH HEMIVERTEBRA
David Arhmashenebeli Georgian University, Tbilisi State Medical University,
University “Geomedi”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.63-65
The study aims to determine the correlations for the physical development with the results of surgical treatment scoliosis in patients with hemivertebra
The study included 125 patients between the ages of 5 to 20 due hemivertebrae scoliosis of the spine.Correlation analysis showed that the unsatisfactory outcome of a significant positive correlation exhibits a height retardation r=0.254**; p=0.004.
11. N. KAPANADZE, I. CHKHAIDZE, I. PANTSULAIA
CYTOKINES AS MARKERS OF PROGNOSIS AND SEVERITY OF PEDIATRIC PNEUMONIA
V. Bakhutashvili Institute of Medical Biotechnology, Tbilisi State Medical University;
M. Iashvili Children’s Central Hospital, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.65-76
The burden of community-acquired pneumonia (CAP) in children is substantial in low- and middle-income countries. Despite a 25% reduction in total cases progressing to severe episodes in the past decade, childhood pneumonia is still one of the leading causes of death. One of the leading causes of morbidity and prolonged course of pneumonia is a change of immunological reactivity. However, the nature of the disturbances of immunity, cell-cell interaction factors insufficiently studied. In this connection it is interesting to study the cytokines that act as mediators of the immune system. They regulate the power, duration and nature of the immune response of an inflammatory process, providing positive and negative immunoregulation.
The recent works indicate that immunological markers may provide supplemental information to clinical findings and assessments. The results may also show that the inflammatory profile correlate stronger to the clinical signs of severity and age than to the certain viruses detected in this study. Consequently, the purpose of this review is to discuss the clinical and immunological disorders and changes in the system of cytokines in patients with extra-hospital pneumonia, assess their impact on the severity of the disease and the rationale for the use of immunotherapy.
12. N. MIRZIKASHVILI, L. BARAMIDZE, I. MZHAVANADZE
HEALTH RISK FACTORS AMONG ADOLESCENTS AND PRIMARY HEALTH CARE ROLE
Tbilisi State Medical University; Ilia State Universiti, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.77-82
The goal of the study was to evaluate health risk factors, specifically tobaccouse and alcohol consumption among adolescents and primary health care role in health promotions activities.
The quantitative survey was conducted among 11-19 years old adolescents. Overall 1000 young people were interviewed across Georgia using a standard questionnaire in 2014. The survey sampling methodology was multi-stage probability sampling. All information were analyzed in SPSS 21 version.
According to survey results more than one third of respondents smoking tobacco ad more than 90% tried alcohol at least once. It is very important that survey results are in line with international surveys’ outcomes that were conducted recently in Georgia. It is very essential that health promotion topics should be implemented at primary health care level, health risk factorsthat influences on adolescent health behaviorsshould be control better way and to increase awareness on risk factors for tobacco and alcohol among adolescents in Georgia.
13. M. GONGADZE, TS. ATAMASHVILI, N.GOGIA, A. SEPASHVILI, M. IOBADZE
INTERLEUKIN-10 AS A REGULATOR OF DIABETES-INDUCED PREGNANCY LOSS
TSMU, V.Bakhutashvili Institute of Medical Biotechnology; P. Shotadze Tbilisi Medical
Academy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.52-88
Beside the high rate of spontaneous abortions, babies are at increased risk of congenital malformations, perinatal mortality, macrosomia and related complications during maternal diabetes type 1 and type 2. The aim of our study is to evaluate therole of IL-10 in the uterus in diabetes induced pregnancy loss. To clarify this question, we performed the study in streptozotocin (STZ) treated FVB femalemice. To investigate influence of IL-10 administration on pregnancy loss in diabetic mice depending on severity of diabetes, mice were injected i.p. with 50 mg/kg interleukin-10. Therefore, pregnancy rate and resorptions quantity were investigated on day 9 of pregnancy, when implantation period is completed. To characterize influence of IL-10 administration on expression of IL-10, TNFα, LIF, NO and MMP-9 in the uterine cells of diabetic mice, we analyzed uterine lysates of diabetic mice collected on days 5 and 7 of pregnancy, encompassing the period of implantation.
In conclusion, the data presented in this article suggest that IL-10 and TNFα play a central role in the pathogenesis of diabetes- induced pregnancy loss. TNFα act as a mediator of diabetes-induced embryotoxic stimuli leading to the death of peri-implantation stage embryos and IL-10 as a suppressor of diabetes-induced abortion in this stage. In addition, our results suggest that molecules such as NO, LIF and MMP-9 may be critical players in the mechanisms determining the outcome of diabetes-induced embryopathic stress.
14. I.ANDRONIKASHVILI, G.SIMONIA, I.PANTSULAIA, A.TAVARTKILADZE, N.PANTSULAIA
THE ROLE OF CARDIOSTREOIDS AND GENETIC FACTORS IN THE DEVELOPMENT OF SALT-SENSITIVITY AND SALT-SENSITIVE HYPERTENSION IN GEORGIAN SUBJECTS
Tbilisi State Medical University - Department of Geriatric,
V.Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.89-95
According to numerous studies genetic factors might play the key role in determining the response of blood pressure to sodium intake. Clinical and experimental research suggests possible multi-level involvement association of cardiosteroids (EO and MBG) in the development of salt-sensitivity in normotensive subjects and in pathogenesis of salt-sensitive hypertension.
The goal of our study was to elucidate the role of genetic factors (ABCB1, CyP3A5 and CyP11B2 genes) and cardiosteroids (EO and MBG) in the development of salt-sensitivity and salt-sensitive hypertension in Georgian patients.
A total of 72 ethnically Georgian middle-aged subjects (22 normotensives and 50 hypertensives) of stage I essential hypertension (JNC VIII) were examined. All subjects were tested for salt-sensitivity.Genetic testing for ABCB1, CyP3A5 and CyP11B2 polymorphisms was preceded by DNA extraction with QIAGEN DNA Blood Mini Kit.Plasma and urine levels of MBG and EO were measured using the ELISA and RIA methods.
Results of our study showed that in Georgia both healthy and hypertensive subjects consume high amount of sodium. Based on our results we would suggest that high incidence of hypertension in Georgia might be related to prevalence of salt-sensitivity in both normotensives and hypertensives. High incidence of salt-sensitivity related genotypes (CYP3A5 3*/3* and ABCB1 CT) in Georgian population might serve as a markers for identification risk groups for the development of salt-sensitive hypertension and provide useful tool for its primary prevention and rational treatment. In Georgians prevalence of salt-sensitivity exceeds average values and development of salt-sensitivity and salt-sensitive hypertension might be due to increased production of cardiosteroids.
15. M. GONGADZE, N. GOGIA, D. CHKHIKVISHVILI, I. CHKHIKVISHVILI
INFLUENCE OF CAUCASIAN RHODODENDRON (RHODODENDRON CAUCASICUM PALL.) LEAF EXTRACT ON LPS-ACTIVATED MURINE PERITONEAL MACROPHAGES
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.95-100
The aim of our study is to evaluate the mechanisms of anti-inflammatory property of Caucasian Rhododendron leaf extract. A number of in vitro assays were performed to evaluate the antioxidant and anti-inflammatory activities of water extracts from the leaves of Rhododendron caucasicum Pall.
MTT assay was performed to determine the effect of Rhododendron extracton cell viability in peritoneal macrophages activated with 5 μg/mL of LPS.We investigated the effect of Rhododendron extract on LPS-stimulated levels of NO,TNF-α and IL-10 productionby Griess reaction based colorimetric assay and ELISA respectively.
Our results demonstrated that rhododendron extract protected peritoneal macrophages and increased their viability. Also, RhEhad a strong inhibitory effect on NO secretionin LPS-stimulatedmacrophages. These results suggest that RhE could be developed as an anti-oxidant agent derived from natural products.
16. B.KORSANTIA, S.KOUPRADZE, M.AKHVLEDIANI, TS.GAGNIDZE, V.MALATSIDZE
NATURAL ZEOLITES IN MEDICINE: IMMUNO-MORPHOLOGICAL EFFECTS OF CLINOPTILOLITE IN THE DIGESTIVE SYSTEM
Tbilisi State Medical University, V. Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.101-107
The purpose of our work was to study pharmacodynamics of the clinoptilolite and his immunotropic properties, also estimation clinical condition of patients with some diseases of the gastrointestinal tract on the background of the zeolite “diet”.
The preparation possesses a strong protective action on organism of patients: observed weakening of dyspeptic effects, the termination of asthenic-vegetative syndrome, reducing the size and normalization of the liver function test, rapid relief of intoxications, decrease in terms of hospitalisation of patients. Zeolite “diet” has been followed by the improvement of functional indices of blood and normalization of the immunological parameters.
Zeolite “diet” raises immunocompetence of organism, especially interferon system. The results of these experiments showed that the particles of clinoptilolite (in the forage) are able to induce a more active immune-morphogenesis in intestinal tissues, liver, regional lymph nodes, spleen and thymus.