J.EXPERIMENTAL & CLINICAL MEDICINE
2017, ¹ 2
1. S. CHINCHARADZE, Z. VADACHKORIA, I. MCHEDLISHVILI
TREND OF DISTRIBUTION OF CLEFT LIP AND PALATE IN GEORGIA
TSMU, Dept of Surgery Direction of Child and Adult Stomatology (Dentistry) and
Prevention of Stomatological (Dental) Diseases; Dept of Epidemiology
and Biostatistics Direction of Public Health, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.21-23
The frequency and trend of distribution of cleft lip and palate in Georgia was studied for years 2006-2015. It was established that in the analyzed period this abnormality had decreasing trend in the country and the minimal number of cases occurred in 2014: 0.76/1000 newborns. The similar situation was seen in the capital of the country Tbilisi and in the Eastern Georgia, excluding Tbilisi. The situation was different in the Western Georgia where this abnormality was seen the least frequently. On this territory there was no decreasing trend. In years 2006-2010 the average rate of cleft lip and palate was 0.62/1000, in years 2011-2015 it became 0.65/1000.
2. M.P. TSIMAKURIDZE, D.G. ZURASHVILI, N.A. KHACHAPURIDZE, MAIA P. TSIMAKURIDZE, L. SH. BAQRADZE, E. A. MAISURADZE
MODERN ASPECTS OF PREVENTION OF OCCUPATIONAL PATHOLOGY
CAUSED BY MANGANESE
Department of Environmental and Occupational Medicine, Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.24-26
The aim of the work is to determine the major sensitive criteria of immunologic and genetics antigens that allow timely discovery of negative influence of manganese on organism and assist in early diagnosis of primary stages of occupational pathology caused by manganese.
The workers of Georgian mining and processing of manganese were observed. Also we have studied professional groups of workers having contact with manganese aerosols.
It was distinguished that,
- Protracted professional contact with manganese causes increase of occupational pathology.
- Upon the studies of people, having a direct contact with manganese, immunological analysis should be given a preference with the aim of revealing the changes in immunological systems, which is one of the first signals for occupational lookout and medical monitoring of the similar contingent.
3. N.KHUNASHVILI, R.KVERENCHKHILADZE, M.TSIMAKURIDZE, L.BAKRADZE,
MAIA TSIMAKURIDZE, N. KHACHAPURIDZE, G. KVERENCHKHILADZE
WORK AND HEALTH OF TBILISI UNDERGROUND WORKERS
TSMU, Department of Environmental Health and Occupational Medicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.26-30
Tbilisi underground workers research has been conducted. It has been estimated that for a number of workers noise abundance, vibration, heat, dust and the existence of other toxic materials increases the risk of occupational disease development.
Once the correlation between working conditions and workers health was established, complex rejuvenating activities were deplaned and developed to improve workplace surroundings.
4. D.TSAAVA, B. ZURASHVILI, M.GIORGOBIANI
GLOBAL WARMING CAUSED BY CLIMATE CHANGE AND POSSIBLE SOLUTIONS TO CURRENT ENVIRONMENTAL ISSUES
TSMU, Faculty of Public Health, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.30-32
Authors presented worldwide organizations work review to slowing and stopping global warmth. This article serving to show the problems of different country and their solution ways. The way to stop the global warmth is communication and experience exchange between countries who is working about elimination of global warmth reasons.
5. D.K.VADACHKORIA, N.D.ASATIANI, M.Z.MURDALASHVILI, T.M.GAMKRELIDZE, L.I.GABUNIA, K.G.GAMBASHIDZE, N.G.PKHALADZE, M.Z.PKHALADZE
CHARACTERISTICS OF MUGGITUSE (DEXTROMETHORPHAN) ADDICTION
Tbilisi State Medical University, Narcological Clinic – Neogene, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.33-36
Despite the increase in consumption of dextromethorphan containing drugs and accompanying problems such as: injuries, violence, deaths, motor vehicle accidents, physical dependence or psychological addiction, in recent years information about the acute intoxication, drug abuse and the methods of treatment against psychoactive containing substance is quite scanty. We were aimed to investigate addiction potential of muggituse and treatment peculiarities. 18-25 years old 8 patients consuming muggituse and other psychoactive remedies (dextromethorphan 200-500; gabapentin 900-1800 mg; baclofen 75-150 mg, during 1-7 months) with the diagnosis of disorders of mental and behavioral disorders were under observation. Results of investigations have shown that although dextromethorphan has mild addiction potential, it must be used carefully in combination with other psychoactive preparations and alcoholics.
6. N. ANTELAVA, M. GHONGHADZE, M. OKUJAVA, K. PACHKORIA
ACTION OF CORVITIN, ACETYLCISTEIN THEIR COMBINATION ON HISTOMORPHOLOGICAL DATA DURING EXPERIMENTAL PARACETAMOL INDUCED ACUTE TOXIC HEPATITIS
Tbilisi State Medical University, Department of Pharmacorherapy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.36-40
The goal of the present experimental study was the evaluation of hepatoprotective action of monotherapy and combined therapy with corvitin and acetylcistein in paracetamol induced hepatotoxicity on the basis of histomorphological alteration during hepatic toxic injury. Experiments were carried out in male Wistar rats weighing 150.0-180.0g. (n=50). Patacetamol-indused hepatic toxicity was revealed in spread necrosis of hepatocytes. Monotherapy with corvitin and acetylcistein and especially their combination was accompanied by high hepatoprotective properties.
Assumingly the hepatoprotective action of corvitin is associated with its antioxidant activity, increasing synthesis of gluthation and reversal in paracetamol toxic metabolite level. It is suggested that in patacetamol-indused acute toxicity the combinacion of corvitin and acetylcistein may be distinguished with high valuability.
7. M. GAGOSHIDZE, A. ZEDGINIDZE, A. PETRIASHVILI, G. PETRIASHVILI,
L. ALADASHVILI, I. TABORIDZE
THE CORRELATION BETWEEN THE LEVEL OF MICRONUCLEI AND THE CONCENTRATION OF ARSENIC IN CHILDREN LIVING IN ARSENIC
Union of Ecological, Social, Medical and Cultural Development ,,Karibche~,
J. Experimental and Clinical Medicine, 2017, 2, pp.41-43
The aim of our work was to detect genetic disorders in children living in arsenic contaminated areas and study the correlation between the level of micronuclei in buccal cells and arsenic concentrations in hear. Were observed 15 children aged 7-15 years living in in the polluted area. As indicator of arsenic exposure was the arsenic concentration in hair. Significant differences with normal indicators were calculated in a ratio of Student. Correlation analysis was performed by Pearson method. Mathematical provision was carried out using the statistical software SPSS 22.
Compared with the control concentration of arsenic increases significantly; 0.5 (t = 2.7, p = 0.0199). The correlation between the number of micronuclei in exfoliated buccal cells and arsenic concentration in hear was- r = 0.890; p = 0.017.
It was determined, that arsenic contaminated area causes the genetic disorders and rising of arsenic concentration in organism, that is dangerous for health and stipulates the necessity of regular health monitoring in polluted area.
8. S. BEZARASHVILI
THE RESULTS OF SELF-ASSESSMENT OF TBILISI ATMOSPHERIC AIR POLLUTION IMPACTS ON THE HEALTH OF TBILISI POPULATION
Department of Environmental Health and Occupational Medicine, Tbilisi State Medical University
J. Experimental and Clinical Medicine, 2017, 2, pp.44-47
Have been studied the impacts of Tbilisi atmospheric air pollution on the population health on the basis of respondents’ self-assessment. As it was concluded, according to 69.2% of the respondents, atmospheric air in Tbilisi is moderately or heavily contaminated. 45.7% of respondents consider that impact of atmospheric air condition on their health is strongly expressed while for 63.8% of respondents the leading position in formation of their health conditions is given to air pollution. The obtained results of the study give the opportunity for developing appropriate prophylactic measures in complex with the other data.
9. VASHAKIDZE N., MEBONIA N., KERESELIDZE M., ZHIZHILASHVILI S.,
GAMBASHIDZE K., ZHIZHILASHVILI A., GARUCHAVA N.
FACTORS AFFECTING PROGNOSIS OF BREAST CANCER IN GEORGIA
1Tbilisi State Medical University, Georgia; 2National Center for Disease Control and Public Health
(NCDC), Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.47-49
Introduction. Breast cancer is the most frequently diagnosed cancer in women worldwide with nearly 1.7 million new cases diagnosed in 2012, in Georgia survival from breast cancer is lower than in many developing countries. The goal of the study was to identify predictors for low survival of disease in the country.
10. E. MIRVELASVILI, A. MAKALATIA, I. BAGHATURIA, E. KIKACHEISVILI
QUALITY OF LIFE IN GROUPS OF PATIENTS LIVING IN TBILISI
Tbilisi State Medical University, Department Public Health, Health management, economic and policy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.49-51
The quality of life is a significant indicator of health. His study was the purpose of our research. The study was conducted in different groups of patients. The groups were divided by the knowledge of health issues. The study showed that there was no significant difference between the groups. The quality of life in groups was average
Methods. Data from population-based cancer registry was used in order to estimate 1-year and 5-years relative survival rates for breast cancer. To identify predictors for low survival time, was performed bivariate analysis. Statistical analyses looked at correlations between possible predictors and survival period.From thecancer registry database 190 breast cancer cases, whose diagnosis were conformed histologically and died in 2015 were included in a statistical analysis.
Results. The results of bivariate analysis, show that late stage at diagnosis (OR=1.89, 95%CI=0.74-4.68), a young age of patients at diagnosis (OR=1.89, 95%CI=0.50–7.17), highly differentiated histological grade (OR=1.21, 95%CI=0.44 - 3.54), is positively correlated with low survival period (less than 5 years), while having mastectomy (OR=0.52, 95%CI=0.27–0.89), adjuvant chemotherapy (OR=0.38, 95%CI=0.20–0.74), and a radiotherapy (OR=0.62, 95%CI=0.31–1.25), have statistically significant positive association with high (more than 5 years), survival period.
Conclusions: modifiable factors, that are predictors for low survival of breast cancer in Georgia are stage of disease at diagnosis and inappropriate treatment. Recommendations: Survival time of Breast Cancer could be increased by early detection and implementation of standardized approaches to management of diseases.
11. L. GABUNIA, SH. KHETSURIANI, N. GAMKRELIDZE, L. SUJASHVILI
PRACTICAL ISSUES OF RESEARCH WITH HUMAN PARTICIPATION
Tbilisi State Medical University, Scientific Research Skills Center, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.51-53
Research with human participation and production of new knowledge in medical field is getting more and more topical throughout the world due to the bio- and chemical terrorism threat, increasing population, spreading infectious diseases, increasing migration and etc.
Involving human subjects in research requires proper justification and following of some biomedical research principles. Among them ethical issues are to be highly considered.
Any research with human participation involves two key principles: research ethics and quality. The inviolability of the participants’ private life and confidentiality of the data should be adequately protected; the results of the research should be widely and easily accessible.
International scientific and ethical standards cover any stage of research – planning, conducting, data obtaining, recording and their reporting. It also provides rights and safety guarantees of research participants; the use of results (positive or negative) in practice implies the improvement of health and well-being of the overall population.
In the context of the health care system, the above-mentioned standard studies are complementary to the health policy in its various aspects; it promotes the development and implementation of higher quality, genuine and equal medical services provision.
12. I. GVILAVA, M. GIORGOBIANI, G. ORMOTSADZE, N. KIPIANI, T. SANIKIDZE 54--56
HEPATOPROTECTIVE ACTIVITY OF CITRUSES POLYMETHYLOXYLATED FLAVONOIDS EXTRACT IN ANIMALS DURING HIGH FREQUENCY
Tbilisi State Medical University; Iv.Beritashvili Center of Experimental Biomedicine, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.54-56
The aim of the research was investigation of the hepatoprotective activity of citruses polymethyloxylated flavonoids extract in animals during high frequency radiation exposure.
In the hepatocytes of irradiated rats the enhanced expression of protein P-50, intensification of apoptosis and necrosis in liver tissue were observed. Citrus extract during radiation-induction damage leads to a slight suppression of proapoptotic protein P53.
13. L.GUMBARIDZE, L.BARAMIDZE, I.ZARNADZE
COST ASSESSMENT AND RELATED ISSUES OF THE UNIVERSAL STATE
HEALTH CARE PROGRAM
Tbilisi State Medical University, Dept of Public Health, Health Care Management, Policy and Health Economy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.58-61
The government of Georgia has developed newuniversal health insurance programsince 2013. In early stages of Program development itis important to make systematic assessment of its strengths and weaknesses and provide recommendations. The goal of the article is the Analysis of country’s health service in the economical point of view, The survey shows that the Established retrospective methods of procurement, lack of development of DRG system increases population demand on medical services, medical service utilization and health spending.
14. I. ZARNADZE, SH. ZARNADZE, L. BARAMIDZE, J. CHEISHVILI, I. EGNATASHVILI MIGRATION PROCESES AND MANAGEMENT OF DEMOGRAPHY CRIZIS
Tbilisi State Medical University, Department of Public Health, Health Care Management, Policy and Economy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.61-64
The social-economical crisis during the last decade in Georgia has been affected by several wars, increasing number of refuges, increasing unemployment, and dramatically increased number of emigrants – defined as the negatively influence on the population of Georgia simultaneously decreasing the demographical development though the Country. According to figures published by the United Nations, if this trend continues, the population will decrease twice by the year 2050, which may result in significant population loss (United the population will decrease twice by the year 2050, which may result in significant population loss (United Nations, 2015).
The aim of scientists and governance coordination and regulation the research of demography processes and implementation new strategy and policy in practice.
15. N. MIRZIKASHVILI, L. BARAMIDZE, I. ZARNADZE
ROLE OF HEALTH CARE PROFESSIONALS INFORMING ADOLESCENTS ABOUT HEALTH PROMOTION ISSUES AT PRIMARY HEALTH CARE LEVEL
Tbilisi State Medical University, Dept of Public Health, Management,
Health Policy and Economy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.67-64
Objectives: The goal of this study was to find out if adolescents receive full information about health related issue and role of primary health care professionals in health promotion.
Methods: The quantitative survey was conducted among 11-19 years old adolescents. Overall 1000 young people were interviewed across Georgia using a standard questionnaire in 2014. The survey sampling methodology was multi-stage probability sampling. All information were analyzed in SPSS 21 version.
Results and Conclusion: According to the study results mostly adolescents do not receive appropriate services and adequate consultation in primary health care centers as it is written in the national and international guideline. Primary health care doctors do not provide full information to young people about reproductive health, healthy lifestyle and health promotion issues. It is necessary to educate adolescents especially face to face consultation is needed. Furthermore, it is important to train health care professionals, namely family doctors and pediatricians in adolescents’ health issues and to implement it in clinical practice.
16. G. LILUASHVILI, I. ZARNADZE, D. KITOVANI, L. LOMTADZE
HISTORY AND PERSPECTIVES OF MILITARY MEDICINE DEVELOPMENT IN GEORGIA
Tbilisi State Medical University, Dept of Public Health, Health care Management,
Policy and Economy, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.67-70
The doctor is the oldest profession in today’s profession and medicine has been developing over the centuries and has played a major role in its development. In the war situation, medical care requires some extra training and skills, for which special care and skills are needed, including “military doctor”, doctor’s assistant, medical orderly, combatant medical orderly and so on. Special treatments as well as management mechanisms are also special. Historically well known fact is the presence of physicians in “Georgians” in the Georgian combat units, this is confirmed by Shota Rustaveli’s immortal poem “Vepkhistkaosani”, where he reminds him that “one slave combat units had to be drawn to me, and he did not pain me. Unfortunately in 2010 the “Military Doctor” of the profession was withdrawn from the National Qualification and Professional Framework of Georgia and the profession was abolished. There are probably no ten countries in the world where military medicine is not present or is not developed, such as Nauru, Andorra, Papua, Grenada, Samoa, Costa Rica, Solomons and Georgia. But in contrast, they have no army. Given the geopolitical situation of Georgia, the profession “military doctor” acquires national importance and it is important to restore military medicine in Georgia and gain regional importance. For this purpose, “National Military Medical Academy of Georgia” was created for the purpose of rehabilitation and rehabilitation of armed forces, higher, medium and supporting medical personnel, to conduct scientific work in military medical direction. The development of military medicine in Tbilisi is conducted in 2017 from Doctoral studies at the Tbilisi State University on the topic “Need, Delivery and Effectiveness, Learning and Evaluation of Health Conditions and Medical Assistance for Military Servicemen and Members of Their Family”.
17. E. SHEKILADZE , T. MDZINARASHVILI, M. KHVEDELIDZE
CALORIMETRIC STUDY OF THE STABILITY OF DPPS-LIPOSOMES
AND C AND E VITAMINS COMPLEXES
Iv. Javakhishvili Tbilisi State University, Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.71-73
The modern nanotechnology was used to encapsulate vitamins (C, E) in DPPC liposomes. Proportions of vitamins and DPPC lipids were selected as one vitamin per one lipid molecule. The hydrophobic vitamin E was located in hydrophobic part of liposome and the hydrophilic vitamin C - on the surface of a bilayer of liposomes’ membrane. The formation of such complex was confirmed with either calorimetric or biological experiments. In particular, calorimetric curve of the complex differs considerably from calorimetric peak profiles of the pure DPPC liposomes, that further emphasize the existence of vitamins and liposomes complex.
18. A.KOTCHLAMAZASHVILI, M. GIORGOBIANI, A. ORKOSHNELI
THE COMBINED USE OF ANTIOXIDANTS – DIOSKORIDI AND GEOMIN IN DYSLIPIDEMIA
Preventive Cardiological Cabinet under Georgia Overseas Friendship International Society,
J. Experimental and Clinical Medicine, 2017, 2, pp.74-76
We conducted outpatient observation of 32 patients (28 men, 4 women) suffered from Hyperlipidemia aged between 34-87. All of them had a high level of cholesterol and triglycerides. We conducted the one-month course of combined antioxidant therapy using Dioskoridi and Geomin in therapeutic doses.
As a result of antilipid treatment in 16 out of 32 patients (50%) the cholesterol level return to normal. As for the elevated levels of low-density, its reduction to the upper limit of normal was observed in 4(12.5%) cases. The increase in high density cholesterol was identified in the same patients at the same time. Andonly 8 patients (25%) were diagnosed with a slight decrease in triglycerides.
This study allows us to make the following conclusions:
1. The combined application of antioxidants: Dioskoridi and Geomin for thirty days improves lipid profile to some extent.
2. Based on natural architectonics of Dioskoridi and Geomin may be freely prescribed for long-term.
3. There is prospect of imminent use of Antioxidants with the ability to act at the cellular level in terms of prevention – treatment of Atherosclerosis.
19. D.TOPHURIA, M.MATOSHVILI, L.BENASHVILI, N.MZAREULISHVILI, I.AMIRANASHVILI
ROLE OF KUPFFER CELLS IN HOST DEFENSE AND LIVER DISEASES
Tbilisi State Medical University, Department of Human Normal Anatomy, Department of Dermato-Venereology, Department of Topographic Anatomy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.76-78
Kupffer cells (KC) constitute 80-90% of the tissue macrophages present in the body. They reside within the lumen of the liver sinusoids, and are therefore constantly exposed to gut-derived bacteria, microbial debris and bacterial endotoxins, known to activate macrophages. Upon activation KC release various products, including cytokines, prostanoides, nitric oxide and reactive oxygen species. These factors regulate the phenotype of KC themselves, and the phenotypes of neighboring cells, such as hepatocytes, stellate cells, endothelial cells and other immune cells that traffic through the liver. Therefore, KC are intimately involved in the liver’s response to infection, toxins, ischemia, resection and other stresses. This review summarizes established basic concepts of KC function as well as their role in the pathogenesis of various liver diseases.
20. N. CHAGANAVA
ON DEPENDENCY OF PHARMACOLOGICAL PROPERTIES OF HETEROARYL CHALKONES ON THEIR STRUCTURES
Department of Medical Chemistry, Tbilisi State Medical University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.78-81
In recent years, various structural modifications of templates of heteroaryl chalcones have been carried out in order to explore their promising biological potential. This article is an effort to summarize design, chemical properties and biological activities of heteroaryl chalcones.
21. N. GORGASLIDZE, M. CHURADZE, K. MCHEDLIDZE, J. ANELI, E. LOMIDZE
HERBARIUM OF TBILISI STATE MEDICAL UNIVERSITY’S I.KUTATELADZE INSTITUTE OF PHARMACOCHEMISTRY – TBPH
Tbilisi State Medical University, Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.81-85
This paper represents 85-year history of the formation and development of the scientific Herbarium of the Institute of Pharmacochemistry, which is specialized mainly as a depot of the flora of the Caucasus. The total amount of collections consists of 35 000 samples - 160 families, 750 genera, 3,300 species of vascular plants. The materials concentrated in the Herbarium in sufficient details reflect the flora of Georgia - 80% of species. 4150 species of plants are indicatedfor Georgian flora.
A rich fund of medicinal plantswas collected over the 85-year period of existence of Herbarium. The unique herbarium specimens are rare and endemic species of all ethnic regions of Georgia and its neighboring countries. Herbarium is a national asset for every civilized country. It is a scientific treasure, which requires long-term conservation, special care, protection and the greatest attention.
Due to importance of the herbarium for the pharmaceutical science, the Herbarium of the Institute of Pharmacochemistry is included in a united international system (the main source of contacts and information) - ,,Index Herbariorum” and is registered in the its ninth edition. The acronym of herbarium is TBPH.
22. T. DARSANIA, B. KURASHVILI, I. ZEDGENIDZE, K. MURJIKNELI
SOME ASPECTS OF THE PREVENTION OF FOOD POISONING IN HOUSEHOLD (AMONG TBILISI POPULATION)
TSSU, Nutritional and Age Medicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.86-88
The increasing number of GI tract infections is detected in Georgia; however complete assessment of cases is not done, because of lack of reports from household. Therefore awareness of population on mandatory hygiene measures will reducerisks of food poisoning associated with; behavior of the population during buying, storing, cooking and preparing food.
The main goal of our studies was evaluation of risks due to food borne diseases/intoxications among population of Tbilisi, by random sampling and using questionnaire. Research covered 120 families, and persons over 18 years old, responsible for cooking and preparing meal at home. Most of respondents noted that they are not informed well about safety of food, preparing, storing and etc. and there is no any guide on hygiene measures and safety of products.
Our studies confirm necessity to create guidebook for consumers as well as for manufacturers, because both are responsible for food safety.
23. L.LOMTADZE, M.KAJRISHVILI, I. ZARNADZE, L.BARAMIDZE, D.KITOVANI, S.CHIRAKADZE
MENTHOL HEALTH AND BASES ASPECTS OF SOCIO-ECONOMICAL
CONDITION OF MIGRANTS
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.88-90
It is well known that migration and health is an increasing problem to the whole world. In this regard, Georgia faces huge challenges. According to the research conducted on this basis, the psychosocial state of such internally displaced people is very important. All the health indicators of these people are much worse. Monitoring and health surveillance of these people is required to solve existing challenges immediately.
24. M. PAPAVA, N. MERKVILADZE, E. EKALADZE, G. MARGVELANI, G. BURJANADZE
CHANGES IN PERIPHERAL MONONUCLEAR CELLS AFTER PROLONGED PSYCHO-EMOTIONAL STRESS
TSMU, Biochemistry department, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 2, pp.91-94
Social and environmental conflicts, irrational distribution of working and resting periods are the characteristic features of modern life which promotes the development of emotional stress.
The aim of our research was to study the changes in the peripheral blood mononuclear cells during stress conditions modeled by social isolation and disrupting of circadian rhythm.
The experiment was conducted on male, white laboratory rats (N=22). Activity of the following enzymes: superoxidismutase, catalase, glutathioreductase and glutathione peroxidase, as well as the concentrations of malondialdehyde and nitric oxide were checked for the evaluation of antioxidant system. The concentrations of melatonin, serotonin, and corticosteron have been determined also.
Our results show the imbalance between antioxidant and prooxidant systems; alongside to the disturbance of circadian rhythm the concentrations of malonedialdehydeand corticosteronare considerably high. More research is needed to give some recommendation for new practical approaches for the diseases caused by emotional stress.