J.EXPERIMENTAL & CLINICAL MEDICINE
2017, ¹ 4
1. 1G. BELKANIA, 2D.KONKOV, 3R.SULUKHIA, 4I.GVINJILIA,5L.DILENYAN, 6L.PUKHALSKA
A NEW LOOK AT THE CIRCULATION IN PREGNANT WOMEN – ANTHROPOPHYSILIGICAL DIAGNOSTICS OF HEMODYNAMIC SUPPORT
1Laboratory of Medical Expert System «Anthropos Systems Lab», Vinnitsa, Ukraine; 2Vinnitsa National Medical University, Ukraine; 3Acad. O.Gudushauri National Medical Center, Tbilisi, Georgia; 4Health Center “Anthropos”, Tbilisi, Georgia; 5Nizhny Novgorod State Medical Academy, Russia; 6Warsaw Medical University, Poland.
J. Experimental and Clinical Medicine, 2017, 4, pp.9-22
The circulatory state of the cardiovascular system (CVS) in pregnant women with physiological pregnancy, with perinatal pathology and in the same age group of nonpregnant ones is considered on the basis of the anthropophysiological approach, which is aimed at bearing pregnancy primarily under typical for humans upright walking conditions. An algorithm for systemic diagnostics of the CVS circulatory state, on the basis of which a new principle of hemodynamic identification of circulatory syndromes of heart failure (HF) is presented, has been considered. The data of the trimester dynamics of the circulatory state of the CVS and the manifestation of the basic syndromes of HF (by perfusion, stagnation) on the left and right heart ventricles, which are determined as a predictor of possible violations of hemodynamic support of pregnancy are shown and discussed.
2. N. SULASHVILI, M. BEGLARYAN
PHARMACISTS’ PROFESSIONAL FEATURES, VIEWED BY THE CUSTOMER’S
(CUSTOMER’S) EYES IN GEORGIA
Yerevan State Medical University, Armenia
J. Experimental and Clinical Medicine, 2017, 4, pp.22-25
Aim and objects is to study professional features of pharmacist.1506 customers (customers) were interviewed by the questionnaire in Georgia. The significance role of the pharmacist is clearly visible from the research. 36.4% of respondents were choosing the drugs by advice of a pharmacist , while choosing a drugstore respondents-50.7% mentioned service culture and 35.9% considers that the most significant factor during choosing drugstore is high quality of personals; respondents-63.1% ask usually to pharmacists about rule of intake of drugs (medications). Respondents -59.2% are required professional competency for a pharmacist. Respondents 82.6% agree that the government should make the certification of pharmacists.
3. G.PATARAIA, T.JORBENADZE, N.JORBENADZE, T.MERMANISHVILI, M.AVALISHVILI
N.CHANTURIDZE, I.PARULAVA, Z.GHACHAVA
MORBIDITY TENDENCY OF TELAVI, GURJAANI AND LAGODEKHI
Iv.Javakhishvili Tbilisi State University, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.25-31
The goal of our research is studying of the late effects of the lead minor, non-toxic doses exposure. We have studied the indicators of spread diseases during 2000-2014 years in Georgia, Telavi, Gurjaani and Lagodekhi, according to the public information of the National Center of Disease Control of the Ministry of Labor, Health and Social Affairs.
After analyzing the materials has been revealed, malignant tumors and ischemic heart disease rates statistically reliable surplus in Telavi and Gurjaani Municipalities, compared with the morbidity average indicators of Georgia. We may suppose that this is the late effects of lead exposure. In addition, it is notable that the statistical analysis of malignant tumors and ischemic heart disease rates has revealed statistically reliable paucity of the same rates in Lagodekhi, than in Telavi and Gurjaani rates. The above mentioned may be considered as one of the arguments for supposition made by us.
Based on mentioned we decided to analyze the records of ontological and cardiology patients’ medical records in Telavi and Gurjaani Municipalities’ population and perform epidemiological research through special questionnaires.
After our inquiries no reliable intraregional difference in food ration and giving priority to any food was detected. Most of the interviewed respondents get natural pectin and less get organic sulfides, that may determine the excessive load of the most of the interviewed respondents with lead ions and development of the existed pathological processes as a result.
Presumably, the regularities after analyzing the collected materials and further high-technology researches may enable new comprehension of various diseases genesis, prevention and treatment tactics.
4.*M.GOGUA, *K.FRANCHUK, **M.NIKOLAISHVILI, *N.OMANADZE
OPTIMIZATION OF THE TREATMENT OF PERIODONTITIS WITH THE APPLICATION OF GRAPE SEED OIL AND THE DRUG STEPALOL-E
David Aghmashenebeli University of Georgia; Biomedicine centre of Iv.Beritashvili, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.31-35
Objective: The study of the therapeutic effect of the inclusion of grape seed oil application and Stepalol-E preparation in the traditional regimen in the treatment of periodontitis.
Material and methods: investigated the effects of grape seed oil application and Stepalol-E preparation on 47 patients and periodontitis and 41 control persons who were treated for periodontitis with the traditional method.
Conclusion: Inclusion of the prerarat of the vomiting bone in the traditional paradontitis therapy regimen is the result of the treatment.
5. TS. ZHORZHOLIANI, L. ZHORZHOLIANI
SOCIAL-HYGIENIC, BIOLOGICAL AND SCHOOL RISK FACTORS AFFECTING HEALTH OF ELEMENTARY SCHOOL AGE CHILDREN (CITY OF BATUMI)
David Aghmashenebeli University of Georgia, Tbilisi
J. Experimental and Clinical Medicine, 2017, 4, pp.35-42
Goal of the research was assessment of the effect of medical-biological, social-hygienic and school internal risk factors on the health of elementary school age children residing in Batumi and identification of the regional priority risk factors.
Observation “case-control” study was performed. Case group included 90 students with morphological-functional disorders and chronic diseases at the remittance stage. Control group included 100 students of I health group with normal physical and psychical development.
The research identified the priority, statistically significant risk factors affecting health of the school age children. Such factors include harmful professions of the parents (OR-1.011), thyroid pathologies (OR-2.778) and obesity (OR-2.094), family history in parents, mothers age over 30 years at a time of childbirth (OR-2.719), delivery pathologies (OR-3.917), high percentage of the behavioral disorders should be specially emphasized: dietary violations (OR-2.562), improper feeding (OR-2.153), excessive fat consumption (OR-2.625), excessive consumption of carbohydrates (OR-2.321) and sleep disorders (OR-2.545).
Awareness in the regional characteristics of the risk factors is of significance with respect of mitigation of the exposure degree and unfavorable effect on the children’s organism, especially regarding that the most of identified risk factors are manageable and subject to correction.
6. T.MIKELADZE, L.ZHORZHOLIANI
CHARACTERISTICS OF WHEEZING PHENOTYPES IN YOUNG CHILD POPULATION
SDASU, Evex diagnostic centre, Clinic “Curacio”,
I.Tsitsishvili Children’s New Clinic, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.42-47
The aim of investigation was to study the clinico-immunological characteristics of various phenotypes of wheezing -syndrome in young children population.
A one-moment, controlled clinical study was conducted in 120 patients, 5-7 years of age. Among them 66.7% had multifactorial wheezing and episodic wheezing - 33.3%, respectively.
According to the study results it was revealed that the phenotype of multifactorial wheezing -syndrome was characterized by: persistent course of the disease; prevalence of night symptoms and elevated total IgE level (427.9-517.5 IU /mL), while the phenotype of episodic wheezing syndrome by: intermitted course of the disease and relatively low total IgE level (161.2-220.1 IU/mL).
Acquaintance with clinical-immunological characteristics of wheezing phenotypes at early age is of great importance to ensure the effectiveness of medical interventions and to reduce asthma burden at early adolescence.
7. N. SULASHVILI
PECULIARITIES OF PROFESSIONAL FOR PHARMACISTS, VIEWED BY THE HEALTH-CARE SPECIALISTS IN GEORGIA
PhD student of Yerevan State Medical University, Armenia
J. Experimental and Clinical Medicine, 2017, 4, pp.47-52
Aim and objects is to study professional peculiarities of pharmacist. 307 health-care specialists were interviewed in Georgia. Majority of health-care specialists considers, that functions are performed by pharmacists in the drug store is to provide information about drugs (medications) to the population. Respondents 45.6% thinks, that level of basic training of pharmacists is not correspond to the contemporary requirements. Majority of health-care specialists considers, that pharmacists are in need of additional- further regular study or training in pharmacology and pharmacotherapy. Majority of health-care specialists considers that it is necessary to provide cooperation between pharmacists and physicians on the issues of pharmacotherapy and considers that government should make the certification of pharmacists.
8. Nona KHARABADZE1, M.ARTMELADZE1, SH.VASHADZE1,3, Nino KHARABADZE2,
E.GOGITIDZE1,3, N.KEDELIDZE3, D.BARATASHVILI1, I.NAKASHIDE3, M.KORIDZE3
CA 125 AND SEX-STEROID HORMONS IN THE BODY OF UTERUS TUMORS
1Oncology Center of the Autonomous Republic of Adjara; 2Batumi Referral Hospital; 3Faculty of Natural Sciences and Health Care, Shota Rustaveli State University, Batumi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.52-56
The body of uterus cancer deserves important attention from women’s oncological disease. Cancer Antigen (CA 125) is the used as marker for women’s gynecological tumors. Also, it’s well established that uterus belongs the most sensitive organ towards sex-steroid hormones. Accordingly, we investigated CA 125 and some sex-steroid hormones (estradiol (E2), progesterone (P) and testosterone (T)) in woman with uterus body (benign and malignant).
9. N.CHIKVAIDZE, N.KINTRAIA, T.MUZASHVILI, G.BURKADZE
FEATURES OF STROMA-PARENCHYMAL INDEX OF CHORION VILLI IN SPONTANEOUS ABORTION
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.56-61
The aim of our study was to identify objective criteria for assessing changes in chorion villi and other constituent components of the placenta.
To assess changes in chorion villus, we introduced a stroma-parenchymal index, which implies the ratio of the total area of the villi (V1) to the area of the villi stroma (V2). (Stroma-parenchymal index V1 / V2).
According to the data obtained, the stroma-parenchymal index with a normal chorion villi is almost exactly one. This means that in the normal chorion villi, the stroma and parenchyma content is almost equal. The highest index of stroma-parenchymal index 4.4 is noted with villous chorion with fibrous stroma and variable for various lesions of villi. There was no significant difference in the stroma-parenchymal index between complete and partial mole, but they differ significantly from normal chorion villus. In complete mole stroma-parenchymal index is 2.9 times greater than the stroma-parenchymal index of the normal chorion villi and at partial mole is 2.6 times greater.
Also important are the correlation of changes in the stroma-parenchymal index of the chorion villi during mole and hydrophilic changes in the villi. With complete mole the stroma-parenchymal index is 1.9 times less than the stroma-parenchymal index with hydrophilic changes in villi, and 1.6 times less with partial mole. In cases of acute and chronic inflammation of chorionic villi, no statistically significant differences were noted.
The stroma-parenchymal index of the chorion villi gives an opportunity for an adequate and objective assessment of the changes in the chorion villi. It can be used as an auxiliary objective criteria for diagnosis of trophoblastic diseases.
10.TOPURIA-NAKAIDZE N.1.2, GIORGADZE I.2, ODISHELIDZE N.3, SABAURI N.1,2,
AKHALAIA E.1.2, TKEMALADZE L.2, KATUKIA T.2
CORRELATION ANALYSES BETWEEN HERPES SIMPLEX VIRUS AND OTHER PATHOGENS CAUSING INFECTIONS IN FEMALES OF REPRODUCTIVE AGE
1G.Eliava Institute of Bacteriophages, Microbiology and Virology;2Bacteriophage Analytical Diagnostic Center ,,Diagnozi 90”;3Eliava Phage Therapy Center, Tbilisi, Georgia.
J. Experimental and Clinical Medicine, 2017, 4, pp.61-65
Diagnosis of the genital infections in females of the reproductive age is essential. Herpes Viruses are characterised by a high risk of reactivation and a fast progress of the infection.
Various microbiologic and immunologic tests allow doctors to study the microflora of the uterus and depending on obtained results to select optimal strategy of treatment.
The results of the study demonstrated that the co-infection of bacterial pathogens and Herpes Simplex Virus, increased the persistence of the virus and its transmission rate.
No clear distinction can be drawn between HSV-1 and HSV-2. Based on the immunological studies it was concluded that in contrast to acute infections, the incidence of chronic infections was much higher (67%).During the study of HSV infections, several patients were also diagnosed with Hepatitis C and HIV that was associated with other polymicrobial infections.
The study is not an alternative to the diagnostic system - TORCH. Despite the fact that this system includes identification of Herpes Simplex virus, our study indicates determination of other (O) infections too.
11. L.LURSMANASHVILI, G.KVARACKHELIA, M.ENUKIDZE, M.MACHAVARIANI,
N.LOBZHANIDZE, T.MCHEDLISHVILI, T.SANIKIDZE
PRO- AND ANTIAPOPTOSIS ACTIVITY OF GREEN TEA KATECHINS
Tbilisi State Medical University; V.Bakhutashvili Institute of Medical Biotechnology;
Georgian David Aghmashenebeli University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.65-69
The effect of green tea leaf extracts on the intensity of apoptosis in intact and incubated under oxidative stress conditions Jurkat cells was studied.
Studies carried out by spectrophotometry (MTT test) and flow cytometry showed that C, and E and E+C vitamins reduce the viability of intact Jurkat cells, but exhibit cytoprotective activity in the case of incubation of cells under oxidative stress conditions.
The extract of green tealeaves, in particular green tea catechins, increases the proliferative activity of Jurkat cells incubated under oxidative stress, but does not affect the viability of intact cells. Green tea pectin exhibits a cytotoxic effect on intact Jurkat cells, but exhibits weak cytoprotective activity on cells incubated under oxidative stress conditions.
12. V. MUNJISHVILI1, T.MUZASHVILI1, M.GACHECHILADZE2, G.BURKADZE1
PROBLEMATIC ISSUES IN THE HISTOPATHOLOGICAL AND MOLECULAR DIAGNOSTICS OF OVARIAN BIRDERLINE TUMORS
1Department of pathological anatomy, tbilisi state medical university, tbilisi, georgia; 2Department of clinical and molecular pathology, Institute of molecular and translational medicine, Palacky University, Olomouc, Czechia
J. Experimental and Clinical Medicine, 2017, 4, pp.69-75
Ovarian borderline tumors represent a separate group of ovarian epithelial tumors, which are characterised by non-invasiveness, heterogeneity and uncertain malignancy potential. There are certain criteria for histopathological diagnosis of ovarian borderline tumors. However, the detection of invasiveness (microinvasiveness) is complicated in some cases.
The molecular and epigenetic markers of borderline ovarian tumors are under investigation. These novel markers will help to determine the full differential diagnosis and prognosis of these in the future.
Ovarian borderline tumors represent the heterogeneous group with various histology and molecular phenotype – so called diagnostic ’’grey zone’’. The determination of biologic behaviour and hence the implementation of relevant treatment-prophylactic means represent the subject of intense investigation in the future.
Shota Rustaveli State University, Batumi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.75-77
Dementia is a general term used to describe a loss of memory that keeps getting worse - or is ‘progressive’. There can be many different causes, they will all involve loss of memory, but they have other symptoms which are different according to the cause. Dementia often starts off with just memory problems, but can also involve: difficulty planning and carrying out day-to-day tasks, difficulty communicating and changes in mood, judgement or personality.
Aim of this article is to identify spread of dementia in 200 patients who have not been diagnosed before . Research was done in ONCO center of Adjara during 2014-2015, involved 200 patients, female - 130, male - 70. For diagnosis of dementia we studied anamnesis, physical data, psychic and cognitive development of patient, family members were questioned often because of the difficulties patients had to explain symptoms they experienced.
All patients had CT scan and evaluated bey Vavilov Method. Arterial Hypertension ,Discirculatory encephalopathy -171, Cancer-5 , Parkinson disease-10 , endocrine disorders-14 , family history of dementia -3 were noted in patients. From 200 patient examined 170 were diagnosed with dementia , Light dementia(10%). experienced memory deterioration Average quality dementia (55%), During severe dementia (35%).
Some risk factors for dementia, such as genetics and age. Cannot be changed. but researchers continue to explore the impact of other risk factors on brain health and prevention of dementia.
14. S. ZEIKIDZE
LEFT VENTRICULAR MODERATE DILATATION PREDICTOR ANALYSIS
IN PATIENTS WITH ASYMPTOMATIC FORM OF ISCHEMIA
David Agmashenebeli University of Georgia, Tbilisi
J. Experimental and Clinical Medicine, 2017, 4, pp.77-81
Aim of the research: To assess risk factors of moderate dilatation in patients with asymptomatic ischemia.
Data and methods: The study is based on test results of 185 patients at the age of 35-55 who were undergoing treatment at the Tbilisi Heart and Vascular Clinic. The patients, among them 117 patients with moderate left ventricular dilatation, did not have typical stenocardial pain. Their results were compared to the ones of 68 patents who did not have such pathological symptoms. Statistical analysis was done using software SPSS 22.
Results: In the moderate dilatation group, the mean ejection fraction was credibly low, corresponding to 49.7±5.6 and 55.2±5.6, whereas, credibly high mass index corresponds to 26.9±2.1 and 29.8±3.6. In the – TCH group of dilatation – 5.6±1.0, in the second group – 4.8±0.9. TG accordingly corresponds to 2.2±0.7 and 1.9±0.5. LDL 3.0±1.0 and 3.5±0.9.
The frequency of the following factors was credibly high in the dilation group: the occupational dust was 0.38 and 0.15, stressful work was 0.54 and 0.29, ischemic disease of the first degree relatives 0.46 and 0.13, hereditary heart diseases – 0.44 and 0.13, residing in the city – 0.44 and 0.25, arterial hypertension 0.71 and 0.44, cardiac failure is 0.29 and 0.13, diabetes mellitus is 0.26 ÓÐ 0.10, tobacco consumption is 0.77 and 0.51.
Conclusion: Predictors of moderate left ventricular dilatation are professional factors, genetic load, tobacco consumption, arterial hypertension, cardiac failure, high weight index, dyslipidemia and diabetes mellitus.
15. M. NATIDZE, S. RIGVAVA
ANTIBIOTIC SENSITIVITY OF ANTHRAX VACCINE STRAINS AND SOME SPECIES BELONGING TO THE GENUS BACILLUS
G. Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia.
J. Experimental and Clinical Medicine, 2017, 4, pp.81-84
Establishment of the antibiotic sensitivity significantly defines therapeutic efficacy of antibiotics and pathogen growth inhibition in the path material.
The researches were designed to study antibiotic sensitivity of some species belonging to the genus bacillus including Anthrax vaccine strains (“STI”, “34F2”, “55” and “Ikhtiman”), Bac. sereus, Bac. anthraciodes, Bac. megatherium, Bac. mesentericus, Bac. subtilis, Bac. Thuringiensis.
The experiments demonstrated a high sensitivity of Anthrax vaccine strains against the following antibiotics: Doxycicline, Bactamed, Dominal, Tetracycline and Cefamezine (4+) which exceeds index of Penicilline (3+, 4+) widely used in dealing with Anthrax.
In addition, resistance of Anthrax vaccine strains and sensitivity of some species belonging to the genus Bacillus against Bactrimforte.
16. N.KOBALADZE, N.GERZMAVA
RISK FACTORS AND ASSESSMENT OF THE TOTAL RISK OF CARDIOVASCULAR DISEASES AMONG ADJARA POPULATION
Caucasus University, Tbilisi; Shota Rustaveli State University, Batumi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.84-94
The prevention of heart attack and stroke through an integrated approach, taking into account the overall cardiovascular risk, is more cost-effective than treating only thresholds for selected risk factors and should be part of a basic package of services to ensure universal coverage of the health - Health care. To achieve this, strengthening of the basic components of the health system will be required, including the financing of medical services to provide access to basic health technologies and essential medicines for the treatment of NCDs.
It should strive to adequately control the main parameters of the modifiable risk factors - body mass index, blood pressure, lipid profile and blood glucose. At the same time, special attention needs to be paid to pharmacotherapeutic correction of the set of target values in order to reduce the risk of developing cardiovascular complications.
It is necessary to increase the attention of specialized outpatient clinicians to identifying and reflecting information on modifiable risk factors for cardiovascular diseases in medical records.
It should strive to expand and optimize the administration of statin therapy with adequate therapeutic doses and longer courses of treatment.
It is necessary to use more widely oral oral anticoagulants according to indications, with mandatory proper monitoring of the hemostasis system.
When prescribing anti-anginal and antihypertensive prophylactic drugs, one should use non-threshold, and at least medial, doses.
More attention should be paid to non-pharmacological correction of risk factors, especially such as overweight and smoking. It is necessary to properly advise the patient about the rational diet and physical activity.
17. B.KORSANTIA, M.SOSELIA, S.KOUPRADZE, TS.GAGNIDZE, V.MALATSIDZE
FUNCTIONAL MORPHOLOGY OF LIVER AND INTESTINE IN MICE UNDER THE ZEOLITE DIET
Tbilisi State Medical University, V. Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.94-97
The purpose of our work was to study pharmacodynamics and influence of the zeolite “diet” (5% food supplement of clinoptilolite) on functional morphology of liver and gastrointestinal tract of white mice.
The used dose of zeolites was non-toxic and lethal to intact mice, has not cause allergic reactions. Zeolite “diet” has been followed by the improvement of glycogen indices in hepatocytes (23,5% more, then in the control), and by the intensification of ion exchange in the small intestine (the amount of ATP was 73% higher). It can be concluded that intracellular regeneration in the liver and intestine is a morphological manifestation of cell activation.
18. M. GONGADZE, N.GOGIA, TS. ATAMASHVILI, A. SEPASHVILI*, M. IOBADZE
THE STUDY OF DIABETES-INDUCED MORPHOLOGICAL CHANGES IN PREGNANT MICE UTERUS
TSMU Institute of Medical Biotechnology, Tbilisi, Georgia
*P. Shotadze Tbilisi Medical Academy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2017, 4, pp.97-103
Perinatal mortality is one of the main complications of diabetic pregnancy. The aim of our study is to evaluate the role of IL-10 in diabetes induced pregnancy loss and investigation of diabetes-induced morphological changes in pregnant mice uterus. To clarify this question, we performed the study in streptozotocin (STZ) treated FVB female mice. To investigate influence of IL-10 administration on pregnancy loss and morphological changes in diabetic uterus depending on severity of diabetes, mice were injected i.p. with 50 mg/kg interleukin-10.
Therefore, pregnancy rate and resorptions quantity were investigated on day 9 of pregnancy, when implantation period is completed and immunohistochemically were detected apoptotic nuclei and proliferation sites in uterus during pregnany. In conclusion, the data presented in this article suggest that IL-10 play a central role in the pathogenesis of diabetes- induced pregnancy loss. IL-10 act as a suppressor of diabetes-induced embryotoxic stimuli leading to the death of peri-implantation stage embryos. IL-10 reverses of diabetes-induced abortion in this stage by modulating morphological changes occurring in diabetic uterus.