J.EXPERIMENTAL & CLINICAL MEDICINE
2019, ¹ 5
1. T.MIKELADZE, L.ZHORZHOLIANI, M.GIGINEISHVILI, L.SAGINADZE, G.ARVELADZE
THE ROLE OF SOCIAL RISK FACTORS IN WHEEZING FORMATION
DURING EARLY CHILDHOOD
Clinic “Curatio”; Institute of Pediatrics Child and Adult Allergy Center; Caucasus Medical Center, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-13-16
The aim of the research was detection of social risk-factors in formation of wheezing during early childhood. Case-control retrospective research was done. In the main group there were 120 patients with multifactorial (80 patients) and episodic (40 patients) wheezing. Control group contained 52 healthy children from 5 to 7 years. They did not have a history of allergy, episode of wheezing and acute infection disease during previous month.
The results of research indicates that statistically reliable social risk factors are: stress during pregnancy (OR-4,83); conflict in the family (OR-1,44) and smoking mother (OR-1,0).
It should be noted, that these risk-factors are manageable and early identification and correction of them allows to prevent asthma.
2. I.PANTSULAIA, N.KAPANADZE, I.CHKHAIDZE
SYSTEMIC CYTOKINE LEVELS IN CHILDREN WITH VERY SEVERE COMMUNITY AQUIRED PNEUMONIA
Tbilisi State Medical University; V.Bakhutashvili Institute of Medical Biotechnology;
M.Iashvili Central Children Hospital; G.Zhvania Pediatric Clinic, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-16-22
The inflammatory response to community-acquired pneumonia (CAP) is orchestrated through activation of pro- and antinflammatory cytokines. However, the findings of studies of cytokines levels in children with acquired pneumonia in the community are contradictory. Consequently, the aim of our study was to scrutinize the relationship between cytokines levels and disease severity. The study includes 62 pediatric patients hospitalized with a diagnosis of pneumonia. Pro- and anti-inflammatory cytokines plasma levels were measured by ELISA.
The results showed that cytokines (IL-8, SNF-alpha, INF-gamma) were significantly higher in patients with severe pneumonia. Also, IL-8 levels correlate with inpatient length of stay and its high concentration is indicative of length of hospital stay. Based on the outcomes we conclude that admission levels of IL-8 and IFN-gamma were associated with disease severity and/or adverse short-term outcome.
3. I.ANDRONIKASHVILI, G.SIMONIA, N.PANTSULAIA
CIRCADIAN RHYTHM OF BLOOD PRESSURE AND SALT-SENSITIVE HYPERTENSION: STUDY IN ELDERLY PATIENTS
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical
Biotechnology; Department of Geriatrics; Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-22-26
It has been known that elderly are a more salt-sensitive subset of the population. Patients in whom BP decreases during the night incur less damage to their brain, kidneys, heart, and blood vessels than people with elevated nocturnal BP. Aging is generally associated with weakening of the circadian system.
The purpose of study was to analyze the relationship between aging, blood pressure variables (including circadian pattern) and salt-sensitivity. The study enrolled a total of 127 (52 middle-aged and 75 elderly) ethnically Georgian patients of stage I essential hypertension (JNC VIII). Anthropometry, BP monitoring, and 24 hr urinary sodium excretion were performed. Determination of circadian rhythm of BP was performed by 24-hr ambulatory blood pressure monitoring.
Our findings confirm results of numerous studies that demonstrate age dependence of salt sensitivity. We found that high salt diet led to significant increase in SBP and MAP only in salt-sensitive hypertensives with peak levels in the elderly. Our results have shown that circadian rhythms of BP are pervasive in salt-sensitive patients, and the degree of this pervasiveness increases with age.
4. G. GURGENIDZE, D. LABARTKAVA
THE ALTERNATIVE METHODS FOR TREATMENT OF BRONCHIAL ASTHMA WITH DECREASED SENSITIVITY TO THE ACTION OF GLUCOCORTICOIDS
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology,
J. Experimental and Clinical Medicine, 2019, 5, pp-26-32
It has been investigated the effectiveness of polyvalent lysates of bacterial origin (Ismigen and Bronchomunal), consisting from antigens, derived via mechanical lysis of eight different bacterial strains of upper respiratory system, for the treatment of steroid-dependent asthmatic patients. After 3 monts,the peroral immunization of patients with polyvalent lysates resulted in significant improvement of their symptoms, reduction of oral and inhaled steroid dosage, decrease in frequency of exacerbations (requiring patients’ hospitalizations) and marked improvement of spirometric values. After ending of treatment these effects were retained at least for 3 months.
5. I.D.CHKHIKVISHVILI, SH.BURCHULADZE, N.N.GOGIA, M.V.ESAIASHVILI
ANTIOXIDANT ACTIVITY AND POLYPHENOL CONTENT IN MIXTURE OF EQUAL AMOUNTS HELIANTHUS TUBEROSUS LEAVES, TILIAE INFLORESCENCES
AND GREEN TEA
TSMU, V. Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-32-38
We studied, consisting in mixture of equal amounts Helianthus tuberosus leaves, Tiliae inflorescences and green tea, retained a high antioxidant activity while decreasing the amount of caffeine. At the same time, enrichment with antidiabetic inulin and chlorogenic acid Helianthus tuberosus leaves from took place. The anti-inflammatory flavonoids of Tiliae inflorescences, with a rather high content of the dominant flavonoids of green tea, epigallocatechin galate, gave the mixture even greater health potential. A mixture of such tea can be successfully recommended for dibetics and patients with chronic diseases. Such a mixture of tea can be used to produce functional products, including fortified bread.
6. B.KORSANTIA, I.KHARISCHARISHVILI, M.AKHVLEDIANI, Ts.GAGNIDZE, E.VOROBIOVA
EFFECT OF GALLERIA MELLONELLA EXTRACT ON THE DEVELOPMENT OF INFLUENZA INFECTION IN WHITE MICE
TSMU, V.Bakhutashvili Institute of Medical Biotechnology. Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-38-45
The effect of the extract from the wax moth larva (Galleria Mellonella) on the antiviral active in influenza infection in white mice was studied. Compared to the control, the dose-dependent lethal animals and virusemia were significantly reduced. The protective effect of the drug also appeared on the model of influenza intoxication. In addition to reducing animal mortality, an improvement in histo-morphological parameters in the lungs, liver, and spleen of mice was recorded.
It can be concluded that the method of biogenic stimulation is a promising direction in the correction of the infectious pathology of the body. Therefore, the wax moth extract we tested can be safely attributed to active natural remedies that can be successfully used for the prevention and treatment of viral and bacterial infections, purulent-inflammatory diseases, as well as other pathological conditions requiring improved metabolic and adaptation processes.
7. S.KUPRADZE, B.KORSANTIA, I.GVELESIANI, L.KAJAIA
SCREENING FOR NEWLY DEVELOPED INFLUENZA VACCINE AGAINST MUTAGENICITY AND EMBRYOTOXICITY
TSMU, Iv.Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-45-48
Our influenza vaccine has been tested for mutagenicity and embryotoxicity in test animals - white mice. It was found that the effect of the vaccine did not differ from the control values in both parameters. These experiments have shown that the newly developed vaccine is safe.
8. N.KUKULADZE, M.ENUKIDZE, M.IOBADZE, M.MACHAVARIANI, A.BAKHUTASHVILI
IZOLATION OF EXOSOMES FROM AMNION MEMBRANE
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-48-52
Discovery of exosomes, small intercellular signaling vesicules, adds a new chapter in the cell biology and regulation of tissuue development and maintenance. It appears that exosomes have a complex and important role in the regulation of different processes in different tissues and whole organism. We describe here some general properties of exosomes and in particular isolation of exosomes from amniotic membrane.
9. 1,2E.KVARATSKHELIA, 2,3T.KVARATSKHELIA, 3M.KAKULIA, 2E.ABZIANIDZE
MTHFR 677TT GENOTYPE AND HYPERHOMOCYSTEINEMIA IN PATIENT WITH SUBCLINICAL HYPOTHYROIDISM, A CASE REPORT
1 Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology;
2TSMU Department of Molecular and Medical Genetics; 3LTD Medical Center “Medimedi”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-52-57
Subclinical Hypothyroidism (SCH) is defined as a condition when the level of TSH increased and circulating thyroxine (T4) and triiodothyronine (T3) concentrations are within the reference range. There are studies demonstrating that plasma homocysteine (tHcy) is high in hypothyroid patients, on the other hand tHcy level is elevated in individuals with MTHFR C677T polymorphism, since the product of MTHFR gene, methylenetetrahydrofolatereductase enzyme involved in homocysteine metabolism.
Here we present the case of 52 years old female who was diagnosed with SCH about 16 years ago (elevated TSH and normal FT4 levels). Hyperlipidemia condition also was confirmed by the lipid analyze. Patient was enrolled to a study related to the link between MTHFR C677T variant and SCH.
MTHFR gene was investigated by the PCR-RELF method using HinfI (NEB Inc) enzyme.
MTHFR 677TT genotype was detected in patient. In addition, tHcy level was significantly elevated (16.7 ìmol/L). After supplementation with folate, B12 and B6 lipid metabolism improved significantly. In addition, we have reduced doses of levothyroxine gradually based on results of plasma TSH, T4and T3levels.
We suggest that molecular studies of MTHFR gene could be valuable for refining the clinical diagnosis of SCH leading to more precise management of this condition.
10. N.UGREKHELIDZE, G.TEVDORASHVILI, T.MUZASHVILI, M.GACHECHILADZE, G.BURKADZE
PROBLEMATIC ISSUES IN PATHOGENESIS OF CHORIOAMNIONITIS
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-57-63
Chorioamnionitis represents the inflammation of amnionic and chorionic membranes, which develops as a result of intraamnionic infeqction. 40-70% of pre-term deliveries are associated with chorioamnionitis, therefore the correct diagnostics and clinical management of the condition is of high importance. Nowadays, the diagnostics of chorioamnionitis are based on standard clinical and histomorphological criteria, whic frequently does not define the severity of the infection, as well as the risk of the develompent of maternal and infant complications. Therefore, the understanding of the molecular mechanisms of the pathogenesis of chorioamnionitis and the identification of novel biomarkers is of crucial importance, which will be used as an additional diagnostic criteria in the future. In the current review we will discuss the problematic issues in the diagnostics of chorioamnionitis.
11. N.CHOMAKHASHVILI, K.FRANCHUK, G.ORJONIKIDZE, Z.CHOMAKHASHVILI, L.KATAMADZE
GENERAL INFORMATION ON ERGONOMICS
David Aghmashenebeli Georgian University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-63-66
The authors discuss in depth the literature on ergonomics, which has implications for various environmental factors and working conditions on the health of the physician, and conclude that adherence to the principles of ergonomics is of particular importance in dentistry, since none of the researches performed by researchers on this topic we did not find valuable work.
12. D.KAJAIA, D. KOCHIASHVILI, T.MUZASHVILI, M.GACHECHILADZE, G.BURKADZE
PROBLEMATIC ISSUES IN MOLECULAR MARKERS OF UROTHELIAL CARCINOMA INVASION AND PROGNOSIS
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-66-72
Urothelial carcinoma of the bladder represents 7th most common cancer in men and 17th most common cancer in women in the world. In 50% of cases after treatment with transurethral resection the disease relapse develops and about 5-25% amongst them progresses into invasive disease after repeated relapse. Histopathological grade remains so far as most important prognostic factor. However, there are many molecular markers which are considered to be useful in defining urothelial carcinoma prognosis and invasive potential. In the current review we will discuss the common molecular markers, which might be used as an additional diagnostic and prognostic criteria in patients with urothelial tumors.
13. 1-2Z.BUKIA, 3N.BERIDZE
CITRUS IN THE SERVICE OF HUMAN HEALTH
1TSMU Institute of Medical Biotechnology, 2TSU Institute of morphology; Tbilisi,Georgia;
3Shota Rustaveli State University, Batumi,Georgia;
J. Experimental and Clinical Medicine, 2019, 5, pp-72-77
A lot herbs deserve attention through the medicine point of view. Many herbal compound play unreplaceable role to strengthen human health.
Citrus must be paid a very special attention in this way. Important issues related to human health is highlighted in this work. Organoleptic indications of getting breeds through selection methods are brought in this work. Their special role is well-founded in medicine- for strengthening the human health.
The idea is underlined that due to methodical selection (hybridization, nuclear selection, clone selection, chemical mutagenesis) the possibility for improvement of citrus organoleptic characteristic is increasing to keep pace in human health service.
14. M.CHIGHLADZE, R.KARSELADZE
THE PECULIARITIES OF NEONATAL ADAPTATION IN THE NEWBORN INFANTS
WITH FETAL GROWTH RETARDATION
I.Javakhishvili Tbilisi State University, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-77-84
The research aimed at studying the structure of the degree and morbidity of neonatal adaptation in the newborn infants suffered from the syndrome of fetal growth retardation.
The cohort of 92 neonates with the syndrome of fetal growth retardation was involved in the study. The degree of neonatal adaptation was evaluated according to the following criteria: the gestation age, the vital and anthropometrical parameters, the degree of asphyxia, the structure of morbidity.
The wide range of neurological and respirational disorders were revealed in the process of research. Compared with the control group (50 neonates), in case of the syndrome of fetal growth retardation the plausible frequency was detected in irritability (P-0.015), the decrease in responsiveness (P-0.000), tremor (P-0.000), the syndrome of seizure (p-0.011) and hypotonia (P-0.000). The asphyxia was identified in 79% (P-0.000) of cases, hypotrophy – 76% (P-0.000) and anemia in 68% (P-0.000).
The findings highlight the problems of neonatal adaptation and health conditions of the neonates with fetal growth retardation, for whose solution the provision of effective and complex medical surveillance assumes the great importance.
15. I.BUCHUKURI13, N.CHKHIKVISHVILI15, M.LORTKIPANIDZE25, G.CHALATASHVILI13, N.LORTKIPANIDZE14
FIXED DRUG REACTION
1S/R National Center of Dermatology and Venereology; 2V.Tsitlanadze Rheumatology S/P Center; 3P.Shotadze Tbilisi Medical Academy; 4D.Tvildiani Medical University; 5European University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-84-91
The aim of the research was to demonstrate the effectiveness of diagnosis of fixed drug reaction using non-invasive methods (clinical signs and symptoms, patient history, dermoscopy and oral test) and recognition of susceptible drug.
Fixed drug reaction is characterized by single or multiple small, dark red to violet patches, that upon resolution leave areas of hyperpigmentation. Morbidity rate around the world varies and possibly is around 2-5% for in-patients and more than 1% for out-patients. B22 and Cw1 antigen is suggestive of genetic predisposition.
Most commonly associated drugs with the disease are: antibiotics, NSAIDs, barbiturates and anti-malarial drugs. The main factor of epidermal damage is an abundance of intraepidermal CD8 T cell in the dermal-epidermal junction, both in active and resolved lesions.
Diagnosis of fixed drug reaction is made according to a patient’s history and morphology of lesions. Potential indications of biopsy are severe systemic symptoms, evolvement into erythrodrema, and/or involvement of mucosal membranes; Provocative tests, such as systemic (oral) and topical (patch-test), are done when disease causing drugs are not certain or multiple drugs were used.
Dermoscopy is a non-invasive diagnostic method, allowing the evaluation of microstructure and pigment distribution in epidermis, dermal-epidermal junction and papillary dermis. It can be used to diagnose the fixed-drug reaction.
Out of the 8 patients we evaluated, 4 of them had a confirmed diagnosis according to their history, 2 by evaluating the skin lesion using dermoscopy, 2 by using the oral test.
HERBAL DRUG ANSALIT – ANTI-LICE TREATMENT
Research department of ”Shroshani Pharmaceuticals”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-91-95
Since insects and microorganisms revealed resistance against conventional chemical drugs and compounds in modern world permanently activated many dangerous defeated deceases of past one of which is pediculosis. According well known modern statistics each year, lice infestations still affect hundreds of millions of people worldwide, 6 to 12 million children in the United States annually. Analysis of data on the global incidence of pediculosis has shown that this remains a major health problem in many countries. Lice involves epidemics of typhus, fever, infestations occur today in many countries despite great efforts to maintain high standards of public health.
During last 10 years scientific circles intensely researching active phitochemicals such as: alkaloids, terpenoids, essantial oils and phenolics from different plants for their insecticidal and antimicrobial activities.
We have to outline that in Georgia there is permanent scientific interest in this direction, we are constantly making efforts on the bases of our endemic flora to create such combination of herbal extracts which will have strong anti-lice activities. Recently we completed preclinical and clinical investigation of herbal drug Ansalit (200ml. for external use). Results are quite satisfying.
17. T.MUZASHVILI, SH.KEPULADZE, M.GACHECHILADZE, G.BURKADZE
PROBLEMATIC ISSUES IN THE PATHOGENESIS OF HASHIMOTO’S THYROIDITIS ASSOCIATED THYROID PAPILLARY CARCINOMA
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-95-100
Thyroid papillary carcinoma represents the most common endocrine malignant tumor. The incidence of thyroid papillary carcinoma is constantly increasing compared to outer tumors. According to the 2015 data of the National Centre of Disease Control the incidence of thyroid cancer in Georgia has moved from 20th place to 2nd place in women. There are numerous risk-factors for the development of thyroid cancer. Particularly interesting is its association with Hashimoto’s thyroiditis, which is considered as one of the risk factors of the development of papillary thyroid carcinoma according to recent studies. However, still there is no definite data about the causative relationship between Hashimoto’s disease and thyroid cancer, also hormone receptors and the subject requires further investigation.
18. E.SHEKILADZE, E.LOMADZE, S.JAPARIDZE, M.ENUKIDZE, M.MACHAVARIANI, I.CHKHIKVISHVILI, T.GABUNIA, M.GOGEBASHVILI, N.IVANISHVILI,
RADIOPROTECTIVE PROPERTIES OF CITRUS POLYMETHOXYLATED
FLAVONOIDS IN MDCK CELLS
TSMU; V.Bakhutashvili Institute of Medical Biotechnology; I.Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2019, 5, pp-100-102
The aim of the study was to investigate the radioprotective properties of citrus polymethoxylated flavonoids in an experimental model of normal epithelial MDCK cells.
The results of the study fconfirm that citrus polymethoxylated flavonoids extract gas dose-dependent radioaprotective axtivity and promoted normalization of normal epithelial MDCK cells viability.