J.EXPERIMENTAL  &  CLINICAL  MEDICINE

2020, № 2

1. N.Yu.BUKULOVA, M.F.DENISOVA

FEATURES OF THE CLINIC AND DIAGNOSIS OF ULCERATIVE COLITIS IN CHILDHOOD ACCORDING TO A RETROSPECTIVE ANALYSIS

State institution «academician O.Lukyanova Institute of Pediatrics, Obstetrics and Gynecology of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine

J. Experimental and Clinical Medicine, 2020, 2, pp-12-17.

This article aims to perform comparative analysis of 100 medical history of children with UC that belongs time period from 2015 to 2019. Also, this article indicates age-related, gender clinical and anamnestic features of ulcerative colitis in children at present and analizes informativeness and relationship of clinical (PUCAI) and endoscopic (Rachmilewitz index) methods of assessing the inflammatory process with ulcerative colitis in children.

Key words: ulcerative colitis, children, risk factors, disease triggers, clinic, diagnosis

2. T.MIKELADZE, M.GIGINEISHVILI, L.ZHORZHOLIANI, L.SAGINADZ, G.ARVELADZE

CONCETRACION OF NITRIC OXIDE IN RELATION TO THE PREDICTIVE INDEX OF ASTHMA (API) AND THEIR PROGNOSTIC SIGNIFICANCE IN EARLY AGE CHILDREN WITH WHEEZING

Clinic “Curatio”; Caucasus Medical Center, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-17-22.

One of the tasks of the study was to investigate the prognostic significance of quantitative changes of Predictive Index of Asthma in children with recurrent wheezing. A study of the Predictive Index of Asthma has shown the efficacy of this method in assessing risk of asthma formation in a population of children with early onset wheezing-syndrome. According to results of API indexthere was selected 65 patients with multifactorial wheezing (81,25%) and 3 patients with episodic wheezing (7,5%), which were revealed that there is high, 77% probability of bronchial asthma formation. this allows us to specify contingencies that require a secondary preventive intervention.

Also the level of nitric oxide concentration was studied in relation with API index. The odds ratio was high and reliable only in 77% of positive cases and equaled 5,92 (95% CI 1.2–39.1, p–0,022). Similarly, high specificity (95%), low sensitivity (24% and high prognostic value (90%) of nitric oxide concentration were observed.

3. B. JAPARIDZE, L.EZEISHVILI

HOW LABOUR CONDITIONS CAN IMPACT ON THOSE OFFSPRING WHO WORK IN SODIUM CYANIDE PRODUCTION

TSMU, The department of environmental health and Occupational medicine;

TSMU, First University Clinic, The department of perinatal medicine

J. Experimental and Clinical Medicine, 2020, 2, pp-22-26.

Within evaluating the impact on reproductive health for those working in sodium cyanide production, their offspring’s health condition were studied. The frequency of complicated pregnancies (for 100 researced) in main group is 1.8 higher compared to control groups. (t=3,19; P<0,001) Main types of complications during pregnancy are: toxic sickness in first and second semester-4.1 high. (t=4,87; P<0,001). The working women in main group 3.24% cases had underweight newborns (about 2000 grams) 3,5 times more compared to control group. The mass and length of newborns in main group reliably falls behind the control group indicators. The specific outcome, first of all must be defined by chemical factors. The results of the conducted research is a basis for future preventive measures.

4. A.TATSKYY¹, A.PECHENKA², M.PRUIDZE³

PROSPECTS FOR THE CLINICAL USE OF CAMELYN® FOR THE TREATMENT AND PREVENTION OF VIRAL INFECTIONS

1–International Institute of Biotherapy; 2–Department of Infectious Diseases P.Shupik,

Kiev, Ukraine; 3–“Camelyn” LLC, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-26-30.

Researchers at the International Institute of Biotherapy (Kiev, Ukraine) conducted a placebo-controlled study of the antiviral efficacy of the substance of the drug Camelyn® on the L929 model using the vesicular stomatitis virus (RNA virus). The data obtained indicate a significant antiviral activity of the drug Camelyn in the conditions of its use against RNA-containing viruses, including the family Rhabdoviridae.

Camelyn’s proven antiviral efficacy against RNA-genomic viruses is important for the prospects of using the drug for actual diseases such as Hepatitis C, Hemorrhagic fever, Coxsackie, Coronavirus infection, seasonal rhinovirus infections. The antiviral effect of the drug Camelyn is mainly due to the activity of natural anti-infectious peptides, the antiviral properties of which were naturally formed during evolution in relation to more evolutionarily ancient RNA viruses.

5. L.BERIDZE¹, T.KATSITADZE², N.KORSANTIA ³, T.EBANOIDZE⁴, N.GRDZELIDZE

PERIORAL DERMATITIS AND HELICOBACTER PYLLORI

¹Batumi Republic Clinical Hospital; ²GidMed plus; ³Tbilisi State Medical University, Department of Dermatology and Venereology, ⁴National Center of Dermatology and Venereology; National Center of Disease Control, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-30-35.

Perioral dermatitis is a multifactorial inflammatory skin condition. It may be aggravated by various factors. An increased incidence of perioral dermatitis has been reported in those who carry the stomach bacterium Helicobacter pylori. Perioral dermatitis is an inflammatory rash involving the skin around the mouth. The rash may spread up to the nose or even the eyes. In that case, it’s referred to as periorificial dermatitis. The purpose of this study was determination of the relationship of Helicobacter pilory infection with perioral dermatitis and to investigate effectiveness of eradication therapy of H.pylori. As our results showed there was correlation between infection rates and perioral dermatitis severity. In most cases the positive test results for H.Pylori was found in patients with moderate to severe form of the disease. The results of the treatment of disease confirmed the effectiveness of the eradication therapy of H.Pylori which will be important to take into account in the therapy of this dermatosis.

6. S.GVAZAVA, V.MARGVELASHVILI, N.CHIKHLADZE

DENTAL TRAUMA AS A PUBLIC HEALTH PROBLEM

I.Javakhishvili Tbilisi State University, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-35-39.

Head and facial injuries are one of the most common causes of morbidity and mortality in developed and developing countries, which can also lead to a temporary or permanent disability. Trauma development can be caused by a variety of factors such as: car accidents, falls, various sports, violence, assaults, domestic and industrial trauma. Facial trauma can lead to scarring, which is accompanied by emotional and psychological problems. Post-traumatic stress disorder, stress syndrome, and depression are usually associated with maxillofacial trauma. Injuries to the face and jaw area play a very important role in a person’s appearance. It also has many important functions, such as eating, breathing, smelling, hearing and speaking.

Dental injuries are also associated with high socioeconomic values and increased hospital costs. Face and jaw injuries are a global problem, they are considered to be health care and socio-economic problem in all countries of the world. This problem affects both developed and developing countries and all age groups of the population. The etiology of facial and soft tissue damage varies between countries and also within the same country. These lesions are associated with serious clinical problems due to their anatomical location. That is why special attention should be paid to etiological factors and mechanisms of trauma. Epidemiological studies are important not only for identifying the burden of trauma, but also for resource management and planning the healthcare sector. This type of research helps us to reduce or even avoid injuries to the jaw area.

7. KHUTSILAVA I., KVLIVIDZE O., PARULAVA TS., MARGVELASHVILI L.

CFTR MUTATIONS AND CLINICAL MANIFESTATION IN CHILDREN DIAGNOSED AFTER NEONATAL SCREENING

New Vision University; I.Tsitsishvili Children Clinic; Cystic Fibrosis Clinical Centre, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-39-44.

Cystic fibrosis (CF) is the most common autosomal recessive disease, which occurs in 1:3000 between Caucasians. The diagnosis of CF is based on compatible clinical findings with biochemical and genetic confirmation. The most common CFTR gene mutation is F508del, which accounts for approximately two thirds of all alleles in patients with CF, with decreasing prevalence from North to Southeast Europe. Some mutations can reach a higher frequency in certain populations. In the past most CF patients were diagnosed after presenting with clinical symptoms. As newborn screening programs become widespread, patients are identified before they become symptomatic.

The aim of study is to report distribution of CF-causing mutations and clinical manifestation in children revealed after neonatal screening. The number of patients diagnosed by neonatal screening, sweat chloride testing and undergo monitoring at cystic fibrosis center during 2013-2019 years was 32. Overall number of patients from this cohort undergo molecular analysis was 22.

We found low frequency of the predominant F508del mutation (4.5%). The most frequent mutation was 1677delTA (43.1%). One mutation (3170C>G) was not previously reported in CF mutation database. In 9 patients with intermediate results of sweat chloride testing CF diagnosis was made after genetic sequencing. We have also carried out genotype-phenotype correlations in this cohort. Most of children were asymptomatic, only 53% had clinical symptoms consistent with CF. The current study presents that newborn screening helps for early identification, earlier intervention and improved outcomes.

8. T.LOMSIANIDZE

RENEWED ANALYSIS OF PHYSICAL DEVELOPMENT OF NEWBORN INFANTS IN GEORGIA

A.Tsereteli Kutaisi State University, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-44-46.

An updated analysis of the physical developmental indicators of newborns was performed again on the basis of data from the Multifunctional Clinic “Beaumonde” including 2012-219. Due to the epidemiological situation, only the 2012 indicators have been fully studied at this stage. The previous complex study was based on data from the same institution.

The results of the analysis showed that the results obtained for the physical development of newborns belong to the average and high data, which gives us basis to assume positive dynamics. The perfect picture will be known as a result of a complex study that includes the last 8 years and further comparisons.

9. L.BERIDZE¹, T.KATSITADZE², N.KORSANTIA³, T.EBANOIDZE⁴, N.GRDZELIDZE⁵

DERMATOSCOPIC DIAGNOSIS AND TREATMENT OF ACTINIC KERATOSIS

¹Batumi Republic Clinical Hospital; ²GidMed-plus; ³Tbilisi State Medical University, Department of Dermatology and Venereology; ⁴National Center of Dermatology and Venereology; ⁵National Center of Disease Control, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-46-49.

Actinic keratosis, also called solar keratosis are photo-induced chronic skin lesions. They are prone to transform into invasive cutaneous squamous-cell carcinomas and are considered as a marker for skin cancer. For diagnosis and differential diagnosis apart visual examination dermatoscopy assessment are important. However, a biopsy may be performed in some cases. A treatment algorithm includes physical and also topical options. Among them are: Cryotherapy and Imiquimod cream. Both are very effective. Also it is important to monitor patients regularly and prescription sunscreens with high potency of protection.

10. I.KHUTSILAVA, O.KVLIVIDZE, Ts.PARULAVA, L.MARGVELASHVILI

THYREOID FUNCTION IN PATIENTS WITH CYSTIC FIBROSIS

New Vision University; I.Tsitsishvili Children Clinic;

Cystic Fibrosis Clinical Center; Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-49-55.

Thyroid dysfunction has been reported in patients with cystic fibrosis (CF) since the 1960, especially when treated with iodine-based expectorants. With iodine-containing expectorants no longer in routine use, the prevalence of thyroid dysfunction in CF patients is unclear.

The aim of study was to detect thyroid status in a cohort of 14 subjects aged 4-16 years. CF diagnose was made with sweat test and molecular analysis. All patients were on enzyme replacement therapy, none of them take iodine-expectorants. There was measured TSH, fT4 and fT3 values, anti-TPO ÓÐ anti-TG in patients with some dysfunction, all patients had thyroid ultrasonography.

Of the 14 subjects 2 had subclinical hypothyroidism and 1 hypothyroidism detected before study and was on thyroid hormone replacement. A positive correlation between age and body mass index and age was not found. Age, sex, BMI, hospitalization status, use of pancreatic enzyme replacement, chronic respiratory infection and recent antibiotic use were not predictive for thyroid dysfunction risk.

Thyroid dysfunction is rare in patients with CF and is likely due to nonthyroidal illness. There are no significant predictors of thyroid dysfunction. Routine investigation is not recommended, but for patients with significant growth failure must be considered.

11. T.LOMSIANIDZE

OBSERVATION OF CERVICAL EROSION TREATMENT RESULTS IN TERMS OF MORPHOLOGICAL CHANGES

Akaki Tsereteli State University, Kutaisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-55-57.

In 2010-2017, the results of cervical erosion treatment were observed in 18 patients in the multifunctional clinic “Beaumonde”. The main criteria for their observation was the thorough examination of the prescribed studies and complex treatment and control for at least 1 year after recovery. At the beginning of the treatment, morphological examination, both colposcopic and biopsy, revealed changes characteristic of the inflammatory processes in the epithelium, ectopics and mild and moderate dysplasias.

Active treatment and regeneration process lasted an average of 8-10 weeks. All patients and their sexual partners followed the indicated recommendations. During the one-year follow-up study, all women experienced complete cure and irreversible morphological changes. Observations show that complex treatment with planned and purposeful treatment has a high solution in terms of normalization of morphological changes.

12. M.E.GOGEBASHVILI, I.R.NOZADZE, N.I.IVANISHVILI, M.G.TSAGARELI

THE FORMATION OF LONG-TERM POST-RADIATION EFFECTS IN WHITE MICE AT THE LEVEL OF PAIN SENSITIVITY

I.Beritashvili Centre of Experimental Biomedicine, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-57-60.

The work considers distant post-radiation effects at the level of formation of pain sensitivity in white mice. Acute gamma radiation with radiocesium (¹³⁷Cs) was used as a radiation factor. It has been shown that a slowdown in the response to irritation can be a result of radiation aging of organisms and related changes in the central nervous system.

14. I.AGHDGOMELASHVILI, B.MOSIDZE, G.MERABISHVILI, S.KAJAYA

EFFECT OF EPIDURAL ANALGESIA ON POSOPERATIVE OUTCOMES OF COLORECTAL SURGERY

High Medical Technology Centre, University Clinic; Tbilisi State Medical University, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-60-64.

The aim of this review was to determine the effects of epidural analgesia in case of colorectal surgery, because it relates to the outcomes after surgery. We reviewed lot of different studies in Pubmed, Medline, Embase and Cochrane library database, that included colorectal surgery and epidural analgesia method. Most of study results demonstrate, that epidural analgesia has a superior effect on pain control after colorectal surgery. Lot of randomized controlled trials have also shown, that it reduces the duration of ileus after colorectal surgery. Data doesn’t show significant effect of epidural analgesia on rates of anastomotic leakage.

15. A.GOKHELASHVILI, M.ENUKIDZE, M.MACHAVARIANI, E.LOMADZE, T.SANIKIDZE

PROGESTERONE PROTECTIVE EFFECT ON MITOGEN-ACTIVATED JURKAT CELLS

Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology, Georgia

J. Experimental and Clinical Medicine, 2020, 2, pp-64-66.

The functional activity of the immune system is regulated by a number of autoregulatory mechanisms that provide maintenance of homeostasis and regulation of the immune response during various diseases through hormone receptors located on the cell surface.

The aim of the study was investigation effect of progesterone on the differentiation and activation of immune cells. The studies were conducted in vitro on a model system of Jurkat cells. Jurkat cells were stimulated with phytogammagglutinin A (DHA) at 5°C for 5 minutes. The viability of Jurkat cells was assessed according examination of the activity of mitochondrial dehydrogenases (MtT test). Research has shown that progesterone exhibits a dose-independent mitigating effect on mitogen-damaging activity.